Now showing items 1-5 of 5

    • Complete biosynthesis of a sulfated chondroitin in Escherichia coli 

      Badri, Abinaya; Williams, Asher; Awofiranye, Adeola; Datta, Payel; Xia, Ke; He, Wenqin; Fraser, Keith; Dordick, Jonathan S.; Linhardt, Robert J.; Koffas, Mattheos A.G. (Nature, 2021-12-01)
      Sulfated glycosaminoglycans (GAGs) are a class of important biologics that are currently manufactured by extraction from animal tissues. Although such methods are unsustainable and prone to contamination, animal-free ...
    • Engineering in vivo sulfation in Escherichia coli for the complete biosynthesis of sulfated glycosaminoglycans 

      Badri, Abinaya (Rensselaer Polytechnic Institute, Troy, NY, 2020-08)
      In this thesis, we attempt to address the demand for animal-free GAGs by engineering single microbial cell factories for complete, one-step GAG biosynthesis. Specifically, we focus on production of chondroitin sulfate (CS), ...
    • Fabrication of homotypic neural ribbons as a multiplex platform optimized for spinal cord delivery 

      Olmsted, Zachary T.; Stigliano, Cinzia; Badri, Abinaya; Zhang, Fuming; Williams, Asher; Koffas, Mattheos A.G.; Xie, Yubing; Linhardt, Robert J.; Cibelli, Jose; Horner, Philip J.; Paluh, Janet L. (Nature, 2020-12-01)
      Cell therapy for the injured spinal cord will rely on combined advances in human stem cell technologies and delivery strategies. Here we encapsulate homotypic spinal cord neural stem cells (scNSCs) in an alginate-based ...
    • N-glycolyl chondroitin synthesis using metabolically engineered E. coli 

      Awofiranye, Adeola E.; Baytas, Sultan N.; Xia, Ke; Badri, Abinaya; He, Wenqin; Varki, Ajit; Koffas, Mattheos; Linhardt, Robert J. (Springer, 2020)
      N-glycolyl chondroitin (Gc-CN) is a metabolite of N-glycolylneuraminic acid (Neu5Gc), a sialic acid that is commonly found in mammals, but not humans. Humans can incorporate exogenous Neu5Gc into their tissues from eating ...
    • The Road to Animal-Free Glycosaminoglycan Production: Current Efforts and Bottlenecks 

      Badri, Abinaya; Williams, Asher; Linhardt, Robert J.; Koffas, Mattheos AG (2018-10-01)
      Animal-extraction, despite its limitations, continues to monopolize the fast-growing glycosaminoglycan (GAG) industry. The past few years have seen an increased interest in the development of alternative GAG production ...