Browsing by Author "Cabral, J.M.S."
Now showing items 1-14 of 14
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Co-culture cell-derived extracellular matrix loaded electrospun microfibrous scaffolds for bone tissue engineering
Carvalho, M.S.; Silva, J.C.; Udangawa, R.N.; Cabral, J.M.S.; Castelo Ferreira, F.; Lobato da Silva, C.; Linhardt, Robert J.; Vashishth, D. (Springer Nature, 2019-06-01)Cell-derived extracellular matrix (ECM) has been employed as scaffolds for tissue engineering, creating a biomimetic microenvironment that provides physical, chemical and mechanical cues for cells and supports cell adhesion, ... -
Compositional and structural analysis of glycosaminoglycans in cell-derived extracellular matrices
Silva, J.C.; Carvalho, M.S.; Han, X.; Xia, K.; Mikael, P.E.; Cabral, J.M.S.; Castelo Ferreira, F.; Linhardt, Robert J. (Springer, 2019-04-15)The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and ... -
Effect of electrical stimulation conditions on neural stem cells differentiation on cross-linked PEDOT: PSS films
Sordini, L.; Garrudo, F.F.F.; Rodrigues, C.A.V.; Linhardt, Robert J.; Cabral, J.M.S.; Castelo Ferreira, F.; Morgado, J. (Frontiers Media SA, 2021-02-12)The ability to culture and differentiate neural stem cells (NSCs) to generate functional neural populations is attracting increasing attention due to its potential to enable cell-therapies to treat neurodegenerative diseases. ... -
Electrical Stimulation of Neural-Differentiating iPSCs on Coaxial Nanofibers
Garrudo, F.F.F.; Nogueira, D.E.S.; Rodrigues, C.A.V.; Ferreir, F.A.; Paradiso, P.; Colaço, R.; Marques, A.C.; Cabral, J.M.S.; Morgado, J.; Linhardt, Robert J.; Ferreira, F.C. (2021-08-07)Neural tissue engineering strategies are paramount to create fully mature neurons, necessary for new therapeutic strategies for neurological diseases or the creation of reliable in vitro models. Scaffolds can provide ... -
Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach
Silva, J.C.; Carvalho, M.S.; Udangawa, R.N.; Moura, C.S.; Cabral, J.M.S.; da Silva, C.L.; Ferreira, F.C.; Vashishth, D.; Linhardt, Robert J. (2020-07-01)The clinical demand for tissue-engineered bone is growing due to the increase of non-union fractures and delayed healing in an aging population. Herein, we present a method combining additive manufacturing (AM) techniques ... -
Extruded bioreactor perfusion culture supports the chondrogenic differentiation of human mesenchymal stem/stromal cells in 3D porous poly (ε-caprolactone) scaffolds
Silva, J.C.; Moura, C.S.; Borrecho, G.; Alves de Matos, A.P.; da Silva, C.L.; Cabral, J.M.S.; Bártolo, P.J.; Linhardt, Robert J.; Ferreira, F.C. (2019)Novel bioengineering strategies for the ex vivo fabrication of native-like tissue-engineered cartilage are crucial for the translation of these approaches to clinically manage highly prevalent and debilitating joint diseases. ... -
Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices using liquid chromatography-tandem mass spectrometry
Silva, J.C.; Carvalho, M.S.; Xia, K.; Cabral, J.M.S.; da Silva, C.L.; Ferreira, F.C.; Vashishth, D.; Linhardt, Robert J. (2020-01-01)Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, ... -
Glycosaminoglycan remodeling during chondrogenic differentiation of human bone marrow−/synovial-derived mesenchymal stem/stromal cells under normoxia and hypoxia
Silva, J.C.; Han, X.; Silva, T.P.; Xia, K.; Mikael, P.E.; Cabral, J.M.S.; Ferreira, F.C.; Linhardt, Robert J. (2020-06-01)Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators ... -
Kartogenin-loaded coaxial PGS/PCL aligned nanofibers for cartilage tissue engineering
Silva, J.C.; Udangawa, R.N.; Chen, J.; Mancinelli, C.D.; Garrudo, F.F.F.; Mikael, P.E.; Cabral, J.M.S.; Ferreira, F.C.; Linhardt, Robert J. (2020-02-01)Electrospinning is a valuable technology for cartilage tissue engineering (CTE) due to its ability to produce fibrous scaffolds mimicking the nanoscale and alignment of collagen fibers present within the superficial zone ... -
PEDOT:PSS-coated polybenzimidazole electroconductive nanofibers for biomedical applications
Sordini, L.; Silva, J.C.; Garrudo, F.F.F.; Rodrigues, C.A.V.; Marques, A.C.; Linhardt, Robert J.; Cabral, J.M.S.; Morgado, J.; Castelo Ferreira, F. (Multidisciplinary Digital Publishing Institute (MDPI), 2021-08-02)Bioelectricity drives several processes in the human body. The development of new materials that can deliver electrical stimuli is gaining increasing attention in the field of tissue engineering. In this work, novel, highly ... -
Polyaniline-polycaprolactone blended nanofibers for neural cell culture
Garrudo, F.F.F.; Chapman, C.A.; Hoffman, P.; Udangawa, R.W.; Silva, J.C.; Mikael, P.E.; Rodrigues, C.A.V.; Cabral, J.M.S.; Morgado, J.M.F.; Ferreira, F.C.; Linhardt, Robert J. (2019-08-01)Neurodegenerative diseases compromise the quality of life of increasing numbers of the world’s aging population. While diagnosis is possible, no effective treatments are available. Using both tissue engineering and ... -
Polyaniline-Polycaprolactone Fibers for Neural Applications: Electroconductivity Enhanced by Pseudo-Doping
Garrudo, F.F.F.; Mikael, P.E.; Rodrigues, C.A.V.; Udangawa, R.W.; Paradiso, P.; Chapman, C.A.; Hoffman, P.; Colaço, R.; Cabral, J.M.S.; Morgado, J.; Linhardt, Robert J.; Ferreir, F.C. (2021-01-01)Replenishing neurons in patients with neurodegenerative diseases is one of the ultimate therapies for these progressive, debilitating and fatal diseases. Electrical stimulation can improve neuron stem cell differentiation ... -
Polybenzimidazole nanofibers for neural stem cell culture
Garrudo, F.F.F.; Udangawa, R.N.; Hoffman, P.R.; Sordini, L.; Chapman, C.A.; Mikael, P.E.; Ferreira, F.A.; Silva, J.C.; Rodrigues, C.A.V.; Cabral, J.M.S.; Morgado, J.M.F.; Ferreira, F.C.; Linhardt, Robert J. (Elsevier, 2019-12-01)Neurodegenerative diseases compromise the quality of life of an increasing number of people in the world's aging population. While diagnosis is possible, no effective treatments are available. Strong efforts are needed to ... -
The effect of electrospun scaffolds on the glycosaminoglycan profile of differentiating neural stem cells
Garrudo, F.F.F.; Mikael, P.E.; Xia, K.; Silva, J.C.; Ouyang, Y.; Chapman, C.A.; Hoffman, P.R.; Yu, Y.; Han, X.; Rodrigues, C.A.V.; Cabral, J.M.S.; Morgado, J.; Ferreir F.C.; Linhardt, Robert J. (Elsevier, 2021-03-01)The use of electrospun scaffolds for neural tissue engineering applications allows a closer mimicry of the native tissue extracellular matrix (ECM), important for the transplantation of cells in vivo. Moreover, the role ...