Browsing by Author "Yan, Lufeng"
Now showing items 1-16 of 16
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Bottom-up analysis using liquid chromatography-Fourier transform mass spectrometry to characterize fucosylated chondroitin sulfates from sea cucumbers
Yan, Lufeng; Li, Lingyun; Li, Junhui; Yu, Yanlei; Liu, Xinyue; Ye, Xingqian; Linhardt, Robert J.; Chen, Shiguo (Oxford University Press, 2019-11-01)Fucosylated chondroitin sulfates (FCSs) from sea cucumbers have repetitive structures that exhibit minor structural differences based on the organism from which they are recovered. A detailed characterization of FCSs and ... -
Chemical O-sulfation of N-sulfoheparosan: A route to rare N-sulfo-3-O-sulfoglucosamine and 2-O-sulfoglucuronic acid
Yan, Lufeng; Brodfueher, Paul; Fu, Li; Zhang, Fuming; Chen, Shiguo; Dordick, Jonathan S.; Linhardt, Robert J. (2020-10-01)Heparosan, the capsular polysaccharide of E. coli K5 is currently used as the starting material in the chemoenzymatic synthesis of heparan sulfate and the structurally related anticoagulant drug heparin. Base hydrolysis ... -
Development of low molecular weight heparin by H2O2/ascorbic acid with ultrasonic power and its anti-metastasis property
Shen, Xuemin; Liu, Zhenfeng; Li, Junhui; Wu, Dongmei; Zhu, Meng; Yan, Lufeng; Mao, Guizhu; Ye, Xingqian; Linhardt, Robert J.; Chen, Shiguo (2019-07-15)Low molecular weight heparins (LMWHs) are currently used as an anticoagulant agent since unfractionated heparin (UFH) can cause serious adverse drug reactions. LMWHs are commercially prepared using different methods such ... -
Effect of the sulfation pattern of sea cucumber-derived fucoidan oligosaccharides on modulating metabolic syndromes and gut microbiota dysbiosis caused by HFD in mice
Li, Shan; Li, Junhui; Mao, Guizhu; Yan, Lufeng; Hu, Yaqin; Ye, Xingqian; Tian, Ding; Linhardt, Robert J.; Chen, Shiguo (2019-04-01)Fucoidans from sea cucumbers are potential functional food ingredients with a well-defined repeating structure. However, there have been fewer function studies of fucoidan oligosaccharides. In the present study, we first ... -
Extraction and characterization of RG-I enriched pectic polysaccharides from mandarin citrus peel
Zhang, Hua; Chen, Jianle; Li, Junhui; Yan, Lufeng; Li, Shan; Ye, Xingqian; Liu, Donghong; Ding, Tian; Linhardt, Robert J.; Orfila, Caroline; Chen, Shiguo (Elsevier, 2018-06-01)Pectin is extensively used as thickener and gelling agent in the food industry. Commercial pectin is usually comprised of homogalacturonans (HG) with small amount of rhamnogalacturonan-I (RG-I). However, recent studies ... -
Fast preparation of rhamnogalacturonan I enriched low molecular weight pectic polysaccharide by ultrasonically accelerated metal-free Fenton reaction
Li, Junhui; Li, Shan; Zheng, Yangfan; Zhang, Hua; Chen, Jianle; Yan, Lufeng; Ding, Tian; Linhardt, Robert J.; Orfila, Caroline; Liu, Donghong; Ye, Xingqian; Chen, Shiguo (2019-10-01)The recovery of pectic polysaccharides with high rhamnogalacturonan I (RG-I) branches from citrus canning processing water was achieved in a previous study aimed at reducing chemical oxygen demand and benefiting both process ... -
Fucosylated chondroitin sulfate oligosaccharides exert anticoagulant activity by targeting at intrinsic tenase complex with low FXII activation: importance of sulfation pattern and molecular size
Li, Junhui; Li, Shan; Yan, Lufeng; Ding, Tian; Linhardt, Robert J.; Yu, Yanlei; Liu, Xinyue; Liu, Donghong; Ye, Xingqian; Chen, Shiguo (2017-01-01)Fucosylated chondroitin sulfates (fCSs) are structurally unusual glycosaminoglycans isolated from sea cucumbers that exhibit potent anticoagulant activity. These fCSs were isolated from sea cucumber, Isostichopus badionotus ... -
Full recovery of value-added compounds from citrus canning processing water
Yan, Lufeng; Ye, Xingqian; Linhardt, Robert J.; Chen, Jianle; Yu, Dandan; Huang, Rui; Liu, Donghong; Chen, Shiguo (2018-03-01)In the production of canned citrus, large amounts of processing water were discharged during the segment membrane removal process, causing severe pollution. In order to reduce pollution and recover the bioactive compounds ... -
Green recovery of pectic polysaccharides from citrus canning processing water
Chen, Jianle; Cheng, Huan; Wu, Dan; Linhardt, Robert J.; Zhi, Zijian; Yan, Lufeng; Chen, Shiguo; Ye, Xingqian (2017-02-15)Canned citrus segments are popularly consumed worldwide with a large global market. However, the production needs chemical treatment to remove segments membranes, producing a large amount of acidic and basic effluents with ... -
Heparan sulfates from bat and human lung and their binding to the spike protein of SARS-CoV-2 virus
Yan, Lufeng; Song, Yuefan; Xia, Ke; He, Peng; Zhang, Fuming; Chen, Shiguo; Pouliot, Robert; Weiss, Daniel J.; Tandon, Ritesh; Bates, John T.; Ederer, Dallas R.; Mitra, Dipanwita; Sharma, Poonam; Davis, April; Linhardt, Robert J. (2021-05-15)Severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2) has resulted in a pandemic and continues to spread at an unprecedented rate around the world. Although a vaccine has recently been approved, there are ... -
Highly purified fucosylated chondroitin sulfate oligomers with selective intrinsic factor Xase complex inhibition
Yan, Lufeng; Wang, D.; Zhu, Mengshan; Yu, Yanlei; Zhang, Fuming; Ye, Xingqian; Linhardt, Robert J.; Chen, Shiguo (2019-10-15)Fucosylated chondroitin sulfate (FCS) oligosaccharides of specific molecular weight have shown potent anticoagulant activities with selectivity towards intrinsic factor Xase complex. However, the preparation of FCS ... -
Molecular size is important for the safety and selective inhibition of intrinsic factor Xase for fucosylated chondroitin sulfate
Yan, Lufeng; Li, Junhui; Wang, Danli; Ding, Tian; Hu, Yaqin; Ye, Xingqian; Linhardt, Robert J.; Chen, Shiguo (2017-12-15)Fucosylated chondroitin sulfate from sea cucumber Isostichopus badionotus (FCS-Ib) showed potent anticoagulant activities without selectivity. The present study focused on developing safe FCS-Ib oligomers showing selective ... -
Oral administration of fucosylated chondroitin sulfate oligomers in gastro-resistant microcapsules exhibits a safe antithrombotic activity
Yan, Lufeng; Zhu, Mengshan; Wang, Danli; Tao, Wenyang; Liu, Donghong; Zhang, Fuming; Linhardt, Robert J.; Ye, Xingqian; Chen, Shiguo (2021-01-01)Fucosylated chondroitin sulfate (FCS) polysaccharide isolated from sea cucumber has potent anticoagulant activity. Based on its resistance to the enzymes present in vertebrates, it may serve as an anticoagulant and shows ... -
Pectic oligosaccharides hydrolyzed from citrus canning processing water by Fenton reaction and their antiproliferation potentials
Li, Junhui; Li, Shan; Liu, Shanshan; Wei, Chaoyang; Yan, Lufeng; Ding, Tian; Linhardt, Robert J.; Liu, Donghong; Ye, Xingqian; Chen, Shiguo (2019-03-01)Citrus canning processing water contains a valuable and renewable source of biopolymers and bioactive compounds including pectic polysaccharides. Upgrading these processing wastes can not only alleviate environmental ... -
Structural elucidation of fucosylated chondroitin sulfates from sea cucumber using FTICR-MS/MS
Agyekum, Isaac; Pepi, Lauren; Yu, Yanlei; Li, Junhui; Yan, Lufeng; Linhardt, Robert J.; Chen, Shiguo; Amster, I. Jonathan (Sage, 2018-02-01)Fucosylated chondroitin sulfates are complex polysaccharides extracted from sea cucumber. They have been extensively studied for their anticoagulant properties and have been implicated in other biological activities. While ... -
Unique cell surface mannan of yeast pathogen Candida auris with selective binding to IgG
Yan, Lufeng; Xia, Ke; Yu, Yanlei; Miliakos, Anna; Chaturvedi, Sudha; Zhang, Fuming; Chen, Shiguo; Chaturvedi, Vishnu; Linhardt, Robert J. (2020-05-08)The emerging, multidrug-resistant yeast pathogen Candida auris is responsible for healthcare-associated outbreaks across the globe with high mortality. The rapid spread of C. auris is linked to its successful colonization ...