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    Individual cell morphological analysis reveals epithelial chiral morphogenesis

    Author
    Raymond, Michael James, Jr.
    View/Open
    175975_Raymond_rpi_0185N_10607.pdf (3.118Mb)
    Other Contributors
    Wan, Leo Q.; Gilbert, Ryan; Wang, Ge, 1957-;
    Date Issued
    2015-05
    Subject
    Biomedical engineering
    Degree
    MS;
    Terms of Use
    This electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.;
    Metadata
    Show full item record
    URI
    https://hdl.handle.net/20.500.13015/1468
    Abstract
    The expression of left-right asymmetry in biological tissue can be described as the chirality in cell alignment. Through development this biologically conserved property can be easily appreciated as the asymmetric positioning of organs and tissues, and asymmetric looping of tissues like the GI tract and the heart. It has been previously reported that various cell phenotypes have an intrinsic, unique, and well-conserved expression of asymmetry, and that changes in chiral expression due to genetic or environmental factors have been linked to tissue malformations and various disease states.; Here, a Python-based algorithm was developed to characterize chiral alignment in epithelial cells through the utilization of immunofluorescence staining and image processing techniques. Straight-lined representations of cell edges were extracted from fluorescence images, and analyzed for morphological features as well as for the alignment and polarization of individual cells and their respective nuclei. These analyses unveiled interrelationships between morphological features and polarization parameters that are impossible to obtain with conventional intensity gradient-based analysis techniques. Utilizing this algorithm, we found that individual cells and nuclei are both chirally aligned and polarized and these alignments and polarizations are conserved, with blunt ends polarized towards the borders. Furthermore, nuclei were shown to position themselves away from micropattern borders as shown in previous literature. On these CCW rings, the cells exhibiting CCW alignment tend to be larger in size and more elongated, when compared to those exhibiting CW alignment, possibly suggesting that the increased front-rear polarization enhances the intrinsic cell chirality. Therefore, individual cell morphological analysis could be a potentially effective analytical tool to reveal the biophysical mechanisms underlying epithelial chiral morphogenesis.;
    Description
    May 2015; School of Engineering
    Department
    Dept. of Biomedical Engineering;
    Publisher
    Rensselaer Polytechnic Institute, Troy, NY
    Relationships
    Rensselaer Theses and Dissertations Online Collection;
    Access
    Restricted to current Rensselaer faculty, staff and students. Access inquiries may be directed to the Rensselaer Libraries.;
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