Investigations into multimodal chromatographic selectivity using in silico designed Fab fragment variants and column modeling
Authors
Karkov, Hanne Sophie
ORCID
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Other Contributors
Cramer, Steven M.
Karande, Pankaj
Tessier, Peter M.
Ahmadian, Haleh
Sejergaard, Lars
Karande, Pankaj
Tessier, Peter M.
Ahmadian, Haleh
Sejergaard, Lars
Issue Date
2015-05
Keywords
Chemical and biological engineering
Degree
PhD
Terms of Use
This electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.
Full Citation
Abstract
The Fab variants were employed to investigate the effects of changing the mobile phase conditions and multimodal ligand design. Using arginine as a mobile phase modifier, Fab variant elution was promoted at a lower ionic strength. In contrast, the presence of guanidine amplified the selective trends of the multimodal system and created a window of selectivity for the separation of Fab variants which could not be separated in the system without the modifier. A novel set of multimodal chromatographic ligands were employed to examine the effects of changing the ligand structure on Fab variant retention and selectivity. Interestingly, the multimodal chromatographic ligands exhibited different selectivity trends and were capable of differentiating between different Fab classes and related variants.
Description
May 2015
School of Engineering
School of Engineering
Department
Dept. of Chemical and Biological Engineering
Publisher
Rensselaer Polytechnic Institute, Troy, NY
Relationships
Rensselaer Theses and Dissertations Online Collection
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