• Login
    View Item 
    •   DSpace@RPI Home
    • Rensselaer Libraries
    • RPI Theses Online (Complete)
    • View Item
    •   DSpace@RPI Home
    • Rensselaer Libraries
    • RPI Theses Online (Complete)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Design and optimization of a bioresorbable polymeric hierarchical ligament fascicle substitute for anterior cruciate ligament tissue regeneration

    Author
    Lee, Kristen Lauren
    View/Open
    176081_Lee_rpi_0185E_10660.pdf (10.12Mb)
    Other Contributors
    Cooper, James A.; Corr, David T.; Plopper, George E.; Ryu, Chang Yeol; Wang, Ge, 1957-;
    Date Issued
    2015-05
    Subject
    Biomedical engineering
    Degree
    PhD;
    Terms of Use
    This electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.;
    Metadata
    Show full item record
    URI
    https://hdl.handle.net/20.500.13015/1498
    Abstract
    There is growing interest in developing a synthetic replacement for the anterior cruciate ligament (ACL). Current replacement options for ACL ruptures utilize autograft or allograft tissues which have limitations that include donor site morbidity, limited supply, risk of disease transfer and immunogenic response after implantation. This "gold standard" leaves room for a synthetic alternative that offers comparable mechanical strength to native tissue, as well as the added possibility of providing chemical cues that promote neo-tissue formation and regeneration of a healthy tissue type.; Knowledge gained from this project contributes to the fields of regenerative medicine and tissue engineering, and in the future can be applied to the development of ligament or tendon replacement scaffolds.; This work demonstrates the production of a synthetic ligament fascicle substitute bundle that is biocompatible and encourages the growth and regeneration of ligament tissue. Research and development utilizes polymer fabrication techniques, extrusion and electrospinning, to create a hierarchical, biomimicing design using poly-l-lactide (PLLA) and polycaprolactone (PCL). The bundle design consists of four individual fascicle substitutes, each with a core extruded PLLA fiber combined with a PCL electrospun nanofiber shell; results show that the mechanical properties of the bundle are dictated by the core fiber material. The novel hierarchical design allows for the incorporation of platelet-derived growth factor (PDGF) and basic fibroblast growth factor (bFGF) for controlled release from the electrospun nanofibers. When cultured with human mesenchymal stem cells (hMSCs), results show that the composite bundle provides a viable substrate for cell attachment and growth. Gene expression shows that the presence of the novel bundle substrate and the presence of growth factors during in vitro culture conditions allows for the upregulation of ligament markers from hMSCs. The known ligament markers: collagen types I and III, tenascin-C and scleraxis, demonstrated an increase in gene expression during 21 day culture conditions.;
    Description
    May 2015; School of Engineering
    Department
    Dept. of Biomedical Engineering;
    Publisher
    Rensselaer Polytechnic Institute, Troy, NY
    Relationships
    Rensselaer Theses and Dissertations Online Collection;
    Access
    Restricted to current Rensselaer faculty, staff and students. Access inquiries may be directed to the Rensselaer Libraries.;
    Collections
    • RPI Theses Online (Complete)

    Browse

    All of DSpace@RPICommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Login

    DSpace software copyright © 2002-2022  DuraSpace
    Contact Us | Send Feedback
    DSpace Express is a service operated by 
    Atmire NV