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dc.rights.licenseRestricted to current Rensselaer faculty, staff and students. Access inquiries may be directed to the Rensselaer Libraries.
dc.contributorDordick, Jonathan S.
dc.contributorKane, Ravi S.
dc.contributorGarde, Shekhar
dc.contributorKeblinski, Pawel
dc.contributor.authorSauer, Jeremy P.
dc.date.accessioned2021-11-03T08:28:03Z
dc.date.available2021-11-03T08:28:03Z
dc.date.created2015-10-01T11:29:42Z
dc.date.issued2015-08
dc.identifier.urihttps://hdl.handle.net/20.500.13015/1524
dc.descriptionAugust 2015
dc.descriptionSchool of Engineering
dc.description.abstractWe first demonstrated in vitro that TRPV1 can be actuated to cause calcium flux into the cell by directly applying a localized magnetic field. In mice expressing these genetically encoded components, application of external magnetic field caused remote stimulation of insulin transgene expression and significantly lowered blood glucose. In addition, we are investigating mechanisms by which iron oxide nanoparticles can absorb RF, and transduce this energy to cause channel opening. This robust, repeatable method for remote cellular regulation in vivo may ultimately have applications in basic science, as well as in technology and therapeutics.
dc.description.abstractMeans for temporally regulating gene expression and cellular activity are invaluable for elucidating the underlying physiological processes and have therapeutic implications. Here we report the development of a system for remote regulation of gene expression by low frequency radiowaves (RF) or by a static magnetic field. We accomplished this by first adding iron oxide nanoparticles - either exogenously or as genetically encoded ferritin/ferric oxyhydroxide particle. These particles have been designed with affinity to the plasma membrane ion channel Transient Receptor Potential Vanilloid 1 (TRPV1) by a conjugated antibody. Application of a magnetic field stimulates the particle to gate the ion channel and this, in turn, initiates calcium-dependent transgene expression.
dc.language.isoENG
dc.publisherRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofRensselaer Theses and Dissertations Online Collection
dc.subjectChemical and biological engineering
dc.titleMagnetogenetics: remote control of cellular signaling with magnetic fields
dc.typeElectronic thesis
dc.typeThesis
dc.digitool.pid176708
dc.digitool.pid176709
dc.digitool.pid176710
dc.rights.holderThis electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.
dc.description.degreePhD
dc.relation.departmentDept. of Chemical and Biological Engineering


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