Compartmentalization of metabolic pathways into peroxisomes of Saccharomyces cerevisiae

Abstract

In the last decade, metabolic engineering benefited greatly from systems and synthetic biology due to substantial advancements in those fields. As a result, technologies and methods evolved to be more complex and controllable than ever. In this thesis, we aimed to improve existing genome engineering technologies to develop controllable and predictable metabolic pathway optimization tool in Saccharomyces cerevisiae. Moreover, we applied up-to-date metabolic engineering pathway balancing techniques to produce drop-in gasoline-replica hydrocarbon biofuels as long-chain alkanes from biological waste, by targeting pathway enzymes into peroxisomes. Lastly, we aimed to engineer the central carbon metabolism of Saccharomyces cerevisiae to develop an acetyl-CoA overproducing strain from biological waste for the production of variety of high-value chemicals and biofuels originated and derived from acetyl-CoA.