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dc.rights.licenseRestricted to current Rensselaer faculty, staff and students. Access inquiries may be directed to the Rensselaer Libraries.
dc.contributorLinhardt, Robert J.
dc.contributorMakhatadze, George I.
dc.contributorColón, Wilfredo
dc.contributorDinolfo, Peter
dc.contributor.authorDickinson, Demetria Marie
dc.date.accessioned2021-11-03T08:37:41Z
dc.date.available2021-11-03T08:37:41Z
dc.date.created2016-08-16T09:00:06Z
dc.date.issued2016-05
dc.identifier.urihttps://hdl.handle.net/20.500.13015/1713
dc.descriptionMay 2016
dc.descriptionSchool of Science
dc.description.abstractChemoenzymatic synthetic methods can provide efficient synthetic routes to heparin-derived oligosaccharides. By combining techniques from traditional organic synthesis and enzymatic catalysis, synthetic routes can be made shorter and with greater yields. One of these techniques is fluorous separations. Tagging an oligosaccharide with a fluorous group and using fluorous affinity chromatography can make the purifications of enzymatic reactions easy. Another technique is the use of unnatural substrates for enzymatic reaction. Several unnatural glycosylation acceptors have been chemically synthesized, and their use as enzymatic substrates in various conditions is explored. Finally, chemoenzymatic techniques have been applied to a synthetic scheme resulting in the pentasaccharide anticoagulant fondaparinux. Several methods for the purification of di-, tri- and tetrasaccharide enzymatic products have been examined and discussed.
dc.language.isoENG
dc.publisherRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofRensselaer Theses and Dissertations Online Collection
dc.subjectChemistry
dc.titleChemoenzymatic synthesis of heparin oligosaccharides
dc.typeElectronic thesis
dc.typeThesis
dc.digitool.pid177367
dc.digitool.pid177368
dc.digitool.pid177370
dc.rights.holderThis electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.
dc.description.degreePhD
dc.relation.departmentDept. of Chemistry and Chemical Biology


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