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dc.rights.licenseRestricted to current Rensselaer faculty, staff and students. Access inquiries may be directed to the Rensselaer Libraries.
dc.contributorWilson, Dwight E.
dc.contributorAuclair, Walter
dc.contributorKrause, Sonja
dc.contributorPfau, Charles J.
dc.contributor.authorStaczek, John
dc.date.accessioned2021-11-03T08:47:05Z
dc.date.available2021-11-03T08:47:05Z
dc.date.created2017-05-04T10:10:28Z
dc.date.issued1976-12
dc.identifier.urihttps://hdl.handle.net/20.500.13015/1916
dc.descriptionDecember 1976
dc.descriptionSchool of Science
dc.description.abstractWhen ATP was omitted from the reaction mixture used in the thymidirte kinase assay, no thymidine monophosphate was formed by the viral or cytosol enzymes. However thymidine monophosphate was formed by the mitochondrial enzyme when ATP was omitted from both the PAGE buffer and the reaction mixture.
dc.description.abstractHeLa cells that have been lytically infected or transformed with Herpes simplex virus Type 1 (HSV-1) have a viral thymidine kinase which is distinct from the cytosol and mitochondrial thymidinekinases. In polyacrylamide gel electrophoresis (PAGE) the mobility of the viral enzyme is intermediate between the cytosol and mitochondrial enzymes. Other workers have added adenosine triphosphate (ATP) to the PAGE buffer to stabilize the enzymes. However, it was found that when ATP was omitted from the PAGE buffer, the activity of the viral and mitochondrial thymidine kinase was not changed, although the cytosol enzyme could not be detected as expected. Apparently, the cytosol enzyme requires ATP for stability, while the viral and mitochondrial enzymes do not.
dc.description.abstractRaji cells contain the Epstein-Barr virus (EBV). The thymidine kinase of Raji cells which had been induced with iododeoxyuridine (Iudr) was examined to determine whether or not an enzyme of viral origin was present. Two peaks of activity were found following PAGE. One peak had a low Rf (0.066) and the second peak had a high Rf (0.233). When ATP was omitted from the PAGE buffert the high Rf activity disappeared; the low Rf activity was still detected. Following inductiont the low Rf activity increasedt but the activity in the high Rf peak was not changed. The thymidine kinases of uninduced and induced Raji cells did not use CTP were not affected by EBV antiserat and were thermostable.
dc.description.abstractHerpes simplex virus Type 2 (HSV-2) also codes for a thymidine kinase which is different from the cytosol or mitochondrial forms of the enzyme. Cells lytically infected with HSV-2 have a viral enzyme with a PAGE mobility intermediate between the cytosol and mitochondrial thymidine kinases. The viral enzyme used CTP as a phosphate donor and was very heat sensitive. Two HSV-2 transformed cell lines showed no evidence of any viral thymidine kinase when tested for their CTP specificity or thermostability. PAGE profiles showed no detectable viral enzyme activity.
dc.description.abstractThe viral and mitochondrial enzymes used cytidine triphosphate (CTP) as a phosphate donor, but the cytosol did not. The viral enzyme was less thermostable than the cytosol or mitochondrial enzymes.
dc.language.isoENG
dc.publisherRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofRensselaer Theses and Dissertations Online Collection
dc.subjectBiology
dc.titleCharacterization of the thymidine kinase in herpes virus-transformed cells
dc.typeElectronic thesis
dc.typeThesis
dc.digitool.pid178092
dc.digitool.pid178093
dc.digitool.pid178094
dc.rights.holderThis electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.
dc.description.degreePhD
dc.relation.departmentDept. of Biology


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