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dc.rights.licenseCC BY-NC-ND. Users may download and share copies with attribution in accordance with a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License. No commercial use or derivatives are permitted without the explicit approval of the author.
dc.contributorMcGown, Linda Baine
dc.contributorWang, Xing
dc.contributorLinhardt, Robert J.
dc.contributor.authorFong, Casey
dc.date.accessioned2021-11-03T08:48:08Z
dc.date.available2021-11-03T08:48:08Z
dc.date.created2017-07-03T14:05:27Z
dc.date.issued2017-05
dc.identifier.urihttps://hdl.handle.net/20.500.13015/1936
dc.descriptionMay 2017
dc.descriptionSchool of Science
dc.description.abstractThe discovery of new DNA aptamers is an arduous process. The primary approach is the Systematic Evolution of Ligands by Exponential Enrichment (SELEX), where many different DNA sequences are screened against a specific target. These targets can vary in size from small molecules to entire cells. Our lab utilizes a novel genome inspired approach, where potential G-quadruplex forming sequences are identified from chromosomal regions, then incubated with human cell extract and screened for selective protein binding. In the past, our group has examined G-quadruplex forming sequences from the ERBB2, c-MYC, VEGF, and RB proximal promoters. The protein nucleolin (NCL) was found to associate with all of them in vitro and in vivo, and the literature has shown that NCL has the propensity to associate with G-quadruplex forming sequences across the human genome. NCL is a protein of particular interest, as it is found on the cell surface of many cancers but is absent in most normal tissues, making the protein a potential biomarker and drug target. This thesis is concerned with creating and testing recombinant NCL to study its G4 interactions, as well as the preparation of a ChIP-exo Illumina Library to identify all of NCL’s genomic binding sites at high resolution in a BT474 cancer cell line. Hopefully this will lead to the discovery of new potential G4 forming sequences that can serve as NCL targeting aptamers.
dc.description.abstractIn recent years, there has been a growing interest in DNA aptamers for both laboratory and clinical applications, finding uses as biosensors, affinity reagents and pharmaceuticals. Aptamers are single stranded oligonucleotides that exhibit high specificity for a single molecular target through their unique, sequence dependent, secondary structures. Among aptamers, the G-quadruplex has been one of the most prominently featured secondary structures.
dc.language.isoENG
dc.publisherRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofRensselaer Theses and Dissertations Online Collection
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.subjectChemistry
dc.titleNucleolin-G-quadruplex interactions, with an emphasis on genome derived sequences
dc.typeElectronic thesis
dc.typeThesis
dc.digitool.pid178159
dc.digitool.pid178160
dc.digitool.pid178161
dc.rights.holderThis electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.
dc.description.degreeMS
dc.relation.departmentDept. of Chemistry and Chemical Biology


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CC BY-NC-ND. Users may download and share copies with attribution in accordance with a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License. No commercial use or derivatives are permitted without the explicit approval of the author.
Except where otherwise noted, this item's license is described as CC BY-NC-ND. Users may download and share copies with attribution in accordance with a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License. No commercial use or derivatives are permitted without the explicit approval of the author.