Browsing Rensselaer Libraries by Subject "Biochemistry and biophysics"
Now showing items 1-20 of 34
-
Analysis of induced differentiation of pluripotent cells
(Rensselaer Polytechnic Institute, Troy, NY, 2011-12) -
Analysis of the functional role of a region in the alpha-core domain of small heat shock proteins
(Rensselaer Polytechnic Institute, Troy, NY, 2007-05) -
Biophysical characterization of seminal amyloid fibrils : interactions with gallic acid and green fluorescent protein
(Rensselaer Polytechnic Institute, Troy, NY, 2019-05)We use PAPf39, SEM1(86-107), and insulin fibrils as our model system for exploring the interaction between green fluorescent protein (GFP) and amyloid fibrils. In order to understand what properties of GFP allows it to ... -
Computational analysis of artificially induced conformational changes and the effects of alternative splicing in Drosophila myosin
(Rensselaer Polytechnic Institute, Troy, NY, 2015-05)The determination of the atomic scale mechanism of protein function is crucial to treatment development for diseased states caused by the malfunction of these proteins. Here, Drosophila myosin is studied using molecular ... -
Computational and structural approaches for the design of green fluorescent protein-based biosensors
(Rensselaer Polytechnic Institute, Troy, NY, 2014-05)Computational and experimental studies are presented with the goal of improving and simplifying the design process. First, a modification to the energy function of the design algorithm is described which optimizes core ... -
Conformational studies of SEM1 monomer and characterization of GFP-amyloid binding
(Rensselaer Polytechnic Institute, Troy, NY, 2019-08)Amyloid fibrils are highly ordered insoluble protein aggregates that are associated with a number of diseases, including Alzheimer’s Disease, Parkinson’s Disease, and Huntington’s Disease. These fibrils can be formed from ... -
Creation of an artificial Golgi organelle : glycosaminoglycans in microfluidics
(Rensselaer Polytechnic Institute, Troy, NY, 2010-05) -
Detailed physicochemical and biological analyses of heparins from various sources
(Rensselaer Polytechnic Institute, Troy, NY, 2015-12)Heparin has been the most widely used anticoagulant medical drug worldwide. However, there are rising concerns about the safety and the steady supply of heparin. International contamination in 2007-2008 resulted in more ... -
Development and application of gel electrophoresis methods for assaying protein kinetic stability
(Rensselaer Polytechnic Institute, Troy, NY, 2019-05)Aside from the functional roles of the hyperstable proteins in these studies, bioinformatic analysis was performed on the identified KSPs to further elucidate structural trends common to the class of proteins. KSPs showed ... -
Drosophila melanogaster & modern protocol analysis for the classroom setting
(Rensselaer Polytechnic Institute, Troy, NY, 2010-12) -
Dynamic regulation of metabolic pathways with CRISPR-dCAS9 toehold-gated rna regulators
(Rensselaer Polytechnic Institute, Troy, NY, 2022-12)In metabolic engineering, once a metabolic pathway is expressed, there is an optimization process that aims to increase the production, titer, rate, and yield of the target molecule with a goal to direct the maximum possible ... -
Enhancing protein activity and stability via scaffold engineering
(Rensselaer Polytechnic Institute, Troy, NY, 2018-05)Rational and computational design methods have been applied to the problem of creating an immunocontraceptive against sperm. Based on the CatSper complex, a voltage gated calcium channel found only in sperm, this contraceptive ... -
Glycosaminoglycans in infectious diseases
(Rensselaer Polytechnic Institute, Troy, NY, 2018-12)Additionally, we evaluated heparin of varying structure for their in vitro potential as antivirals against ZIKV and DENV infection and discovered that heparin surprisingly promotes ZIKV replication in a structure specific ... -
Hyperosmotic shock and recombinant antibody production in CHO cells : characterization and gene expression investigation
(Rensselaer Polytechnic Institute, Troy, NY, 2011-05) -
Insights into the structure, activity, and therapeutic potential of glycosaminoglycans
(Rensselaer Polytechnic Institute, Troy, NY, 2009-05) -
Investigating Na+ binding sites in the Na+-translocating NADH:ubiquinone dehydrogenase (Na+-NQR)
(Rensselaer Polytechnic Institute, Troy, NY, 2018-05)Not much is known about the coupling mechanism between the redox reactions and Na+ translocation. The focus of my thesis work has been to investigate specific residues involved with Na+ uptake as well as proposed pathways ... -
Investigating the indentification and construction of therapeutic glycosaminoglycans
(Rensselaer Polytechnic Institute, Troy, NY, 2013-05)Using the heparin biosynthetic pathway as a model, three small-scale techniques were tested: milligram bench-top synthesis, channel microfluidics via SPR, and digital microfluidics. The target structure was a specific ... -
Investigating the mechanistic difference between the D46 and H46 of the hedgehog hint domain in hedgehog autoprocessing
(Rensselaer Polytechnic Institute, Troy, NY, 2018-05)The overexpression of the Hedgehog signaling pathway is involved in the development of many cancers. The pathway is activated by the autoprocessing of the Hedgehog precursor, without the need of any accessory protein. ... -
Kap is a neuronal organelle adaptor for KIF3AB and KIF3AC
(Rensselaer Polytechnic Institute, Troy, NY, 2022-12)Neurons are the fundamental unit of the nervous system, their function being to receive and propagate electrochemical signals. Due to this role, neurons can be exceptionally large cells with specialized subdomains known ... -
Mechanisms of the myosin converter
(Rensselaer Polytechnic Institute, Troy, NY, 2018-05)To test this hypothesis, we utilized the unique properties of the Drosophila jump muscle system which enables both genetic modification of the converter and the measurement of the muscle FVR. In Drosophila, there are five ...