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    Absolute protein quantitation reveals a nutrient-dependent link between cell growth and division

    Author
    Dorsey, Savanna Braam
    View/Open
    179308_Dorsey_rpi_0185E_11335.pdf (9.791Mb)
    Other Contributors
    Royer, Catherine Ann; Barquera, Blanca L.; Collins, Cynthia H.; Hurley, Jennifer;
    Date Issued
    2018-08
    Subject
    Biology
    Degree
    PhD;
    Terms of Use
    This electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.;
    Metadata
    Show full item record
    URI
    https://hdl.handle.net/20.500.13015/2286
    Abstract
    How a cell knows when it has grown large enough to divide is a fundamental unanswered question with broad implications to understanding disease. Cell size homeostasis, or the balance between growth and division, is modulated by growth factors in mammalian cells and by nutrients in the model organism, budding yeast, such that cells grown in rich nutrients are larger than those grown in poor nutrients. Although decades of investigation and hundreds of papers have been put forth seeking to uncover the missing link between the distinctly regulated processes of growth and division, progress has been hampered by genetic complexity and an overwhelming amount of qualitative data , lacking complementary reproducible quantitative data. Implementing a particle-counting fluorescence-fluctuation technique in live single yeast cells in respect to cell growth and time, we determined absolute quantification of key proteins regulating the commitment to cell division at the G1/S cell cycle transition, termed Start. We found the number of G1/S transcription factors is growth and nutrient dependent, and the ratio of transcription factors to number of promoter sites of the G1/S regulon is a key determinant of Start. This work explains in part how cell growth and extracellular nutrient conditions dictate at what size a single yeast cell commits to divide.;
    Description
    August 2018; School of Science
    Department
    Dept. of Biological Sciences;
    Publisher
    Rensselaer Polytechnic Institute, Troy, NY
    Relationships
    Rensselaer Theses and Dissertations Online Collection;
    Access
    Restricted to current Rensselaer faculty, staff and students. Access inquiries may be directed to the Rensselaer Libraries.;
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