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dc.rights.licenseRestricted to current Rensselaer faculty, staff and students. Access inquiries may be directed to the Rensselaer Libraries.
dc.contributorRoyer, Catherine Ann
dc.contributorBarquera, Blanca L.
dc.contributorCollins, Cynthia H.
dc.contributorHurley, Jennifer
dc.contributor.authorDorsey, Savanna Braam
dc.date.accessioned2021-11-03T09:05:24Z
dc.date.available2021-11-03T09:05:24Z
dc.date.created2018-10-24T13:40:46Z
dc.date.issued2018-08
dc.identifier.urihttps://hdl.handle.net/20.500.13015/2286
dc.descriptionAugust 2018
dc.descriptionSchool of Science
dc.description.abstractHow a cell knows when it has grown large enough to divide is a fundamental unanswered question with broad implications to understanding disease. Cell size homeostasis, or the balance between growth and division, is modulated by growth factors in mammalian cells and by nutrients in the model organism, budding yeast, such that cells grown in rich nutrients are larger than those grown in poor nutrients. Although decades of investigation and hundreds of papers have been put forth seeking to uncover the missing link between the distinctly regulated processes of growth and division, progress has been hampered by genetic complexity and an overwhelming amount of qualitative data , lacking complementary reproducible quantitative data. Implementing a particle-counting fluorescence-fluctuation technique in live single yeast cells in respect to cell growth and time, we determined absolute quantification of key proteins regulating the commitment to cell division at the G1/S cell cycle transition, termed Start. We found the number of G1/S transcription factors is growth and nutrient dependent, and the ratio of transcription factors to number of promoter sites of the G1/S regulon is a key determinant of Start. This work explains in part how cell growth and extracellular nutrient conditions dictate at what size a single yeast cell commits to divide.
dc.language.isoENG
dc.publisherRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofRensselaer Theses and Dissertations Online Collection
dc.subjectBiology
dc.titleAbsolute protein quantitation reveals a nutrient-dependent link between cell growth and division
dc.typeElectronic thesis
dc.typeThesis
dc.digitool.pid179307
dc.digitool.pid179308
dc.digitool.pid179309
dc.rights.holderThis electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.
dc.description.degreePhD
dc.relation.departmentDept. of Biological Sciences


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