• Login
    View Item 
    •   DSpace@RPI Home
    • Rensselaer Libraries
    • RPI Theses Online (Complete)
    • View Item
    •   DSpace@RPI Home
    • Rensselaer Libraries
    • RPI Theses Online (Complete)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Rational design and study of peptide ligands for carbohydrates : applications in understanding bimolecular recognition and in targeting of biomolecules

    Author
    Shastry, Divya Gowri
    Thumbnail
    View/Open
    179958_Shastry_rpi_0185E_11078.pdf (51.80Mb)
    Other Contributors
    Karande, Pankaj; Linhardt, Robert J.; McGown, Linda Baine; Cramer, Steven M.;
    Date Issued
    2017-05
    Subject
    Biochemistry and biophysics
    Degree
    PhD;
    Terms of Use
    This electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.;
    Metadata
    Show full item record
    URI
    https://hdl.handle.net/20.500.13015/2494
    Abstract
    The ability of biomolecules to recognize one another forms the basis of life as we know it. Beginning from fundamental knowledge on molecular interactions, complex systems can be broken down to understand biological processes, and this knowledge may be exploited for the benefit of humankind – whether in diagnostics, therapeutics, or any number of biotechnological applications. Carbohydrates, which display exquisite recognition skills in biology, offer great potential for study and for use as ligands in this regard. The development of carbohydrate-binding ligands may serve to open up new application spaces in comparison to the more developed protein sphere, while simultaneously providing insights into molecular mechanisms of carbohydrate recognition. The application of rational design principles to such efforts provides the benefit of breaking down the complexities of molecular interaction into rules that can lead to enhanced fundamental understanding of systems in hand.; Peptides as ligands in the targeting of carbohydrates may serve two purposes – to develop novel ligands that exhibit characteristics (like low molecular weight and production ease) amenable for application as well to promote understanding of carbohydrate biological recognition. Specifically, in regard to the latter purpose, peptides offer the possibility to study and exploit natural carbohydrate interactions as the basis for rational design. In this work, carbohydrate-binding peptides were rationally designed against the glycan polysialic acid (PSA), which appears in many interesting contexts, such as influencing nervous system development on neural progenitor cells and promoting tumor cell metastasis. The rational design approach employed through examination of PSA–protein interactions enabled molecular-level understanding of these natural PSA interactions and led to the development of PSA-binding peptides that may be used in biosensing and targeting applications, such as glycan-based targeting of stem cells for regenerative medicine. PSA-binding peptides were shown to demonstrate sequence-dependent specificity, thus leading to the development of rules for glycan recognition by engineered peptide ligands. Ultimately, principles learnt from this body of work may be extended to other carbohydrate systems, leading to such applications as small-molecule targeting of specific glycosylation patterns in healthcare contexts.;
    Description
    May 2017; School of Science
    Department
    Biochemistry and Biophysics Program;
    Publisher
    Rensselaer Polytechnic Institute, Troy, NY
    Relationships
    Rensselaer Theses and Dissertations Online Collection;
    Access
    Restricted to current Rensselaer faculty, staff and students. Access inquiries may be directed to the Rensselaer Libraries.;
    Collections
    • RPI Theses Online (Complete)

    Browse

    All of DSpace@RPICommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Login

    DSpace software copyright © 2002-2022  DuraSpace
    Contact Us | Send Feedback
    DSpace Express is a service operated by 
    Atmire NV