Mass spectrometry analysis of glycosaminoglycans and applications in biological research

Authors
Han, Xiaorui
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Other Contributors
Linhardt, Robert J.
McGown, Linda Baine
Karande, Pankaj
Wang, Xing
Issue Date
2020-05
Keywords
Chemistry
Degree
PhD
Terms of Use
Attribution-NonCommercial-NoDerivs 3.0 United States
This electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.
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Abstract
The glycosaminoglycan (GAG) family of polysaccharides includes the unsulfated hyaluronan and sulfated heparin, heparan sulfate, keratan sulfate, and chondroitin/dermatan sulfate. GAGs are involved in various biological processes such as cell signaling, mediating interactions between cell surface receptors, blood coagulation, tumorigenesis and cancer progression. However, the detailed mechanism of action, the biosynthesis and diversification of GAGs is still largely unknown. A better understanding of GAG composition and the amounts of GAGs within body fluids and tissues can help us gain a more profound understanding of fundamental biological processes and pathology in some diseases. There is a high demand for advanced analytical technologies in the field of GAG related life-science research. The work performed in this thesis is focused on developing and optimizing advanced mass spectrometry analytical methods for GAGs, especially in heparin drug quality control analysis, sepsis disease pathology analysis, and urinary GAGs analysis. Additionally, the use of a novel negative-ion mode isoelectric focusing capillary electrophoresis (iCEF-MS) analytical method is reported for the first time for GAG analysis. In this study, negative-ion mode iCEF-MS has proven capable of successfully separating oligosaccharides with good resolution and high sensitivity.
Description
May 2020
School of Science
Department
Dept. of Chemistry and Chemical Biology
Publisher
Rensselaer Polytechnic Institute, Troy, NY
Relationships
Rensselaer Theses and Dissertations Online Collection
Access
CC BY-NC-ND. Users may download and share copies with attribution in accordance with a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License. No commercial use or derivatives are permitted without the explicit approval of the author.