Enabling scalable electrochemical cofactor regeneration via improved soluble expression and application of mutant renalase
Authors
Morrison, Clifford Steven
ORCID
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Other Contributors
Koffas, Mattheos A. G.
Dordick, Jonathan S.
Chakrapani, Vidhya
Barquera, Blanca L.
Dordick, Jonathan S.
Chakrapani, Vidhya
Barquera, Blanca L.
Issue Date
2020-05
Keywords
Chemical engineering
Degree
PhD
Terms of Use
This electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.
Full Citation
Abstract
Therefore, devising engineering strategies for the improved soluble expression of hRen1 is necessary for enabling the recovery of adventitious reduced NAD(P)H cofactor material from electrochemical cofactor regeneration preparations. In this work, the primary sequence of hRen1 has been mutated and fused to various solubility proteins to enhance soluble expression of the enzyme, and their steady-state kinetics parameters are characterized relative to the wild type enzyme. Additionally, engineering strategies for utilizing electrochemical bioreactors for microbial electrosynthesis applications is explored.
Description
May 2020
School of Engineering
School of Engineering
Department
Dept. of Chemical and Biological Engineering
Publisher
Rensselaer Polytechnic Institute, Troy, NY
Relationships
Rensselaer Theses and Dissertations Online Collection
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