Purification of antibodies using elastin like polypeptide based affinity precipitation
Authors
Bhat, Manish Krishna
ORCID
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Other Contributors
Cramer, Steven M.
Przybycien, Todd M.
Bequette, B. Wayne
Przybycien, Todd M.
Bequette, B. Wayne
Issue Date
2020-08
Keywords
Chemical engineering
Degree
MS
Terms of Use
This electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.
Full Citation
Abstract
With the final aim being the development of a continuous affinity precipitation setup for the purification of mAbs, the first part deals with an improvement to the affinity precipitation process. Optimal conditions for the new elution without resolubilization process are identified by carrying out a high-throughput study in batch mode. We successfully obtain high recoveries above 90% for both the mAbs tested.
Description
August 2020
School of Engineering
School of Engineering
Department
Dept. of Chemical and Biological Engineering
Publisher
Rensselaer Polytechnic Institute, Troy, NY
Relationships
Rensselaer Theses and Dissertations Online Collection
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