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dc.rights.licenseRestricted to current Rensselaer faculty, staff and students. Access inquiries may be directed to the Rensselaer Libraries.
dc.contributorBystroff, Christopher, 1960-
dc.contributorKoffas, Mattheos A. G.
dc.contributorForth, Scott T.
dc.contributorWang, Chunyu
dc.contributor.authorJordan, Thomas Brown
dc.date.accessioned2021-11-03T09:22:46Z
dc.date.available2021-11-03T09:22:46Z
dc.date.created2021-02-22T15:34:24Z
dc.date.issued2020-08
dc.identifier.urihttps://hdl.handle.net/20.500.13015/2636
dc.descriptionAugust 2020
dc.descriptionSchool of Science
dc.description.abstractThe global unplanned pregnancy rate remains high (nearly 50%) despite decades of workto provide contraception to all who want it. Contraceptive availability is good, but manyunplanned pregnancies are the result of contraceptive failure or deliberate non-use. An anti-sperm contraceptive vaccine seems likely to have a low failure rate and to be attractive tocurrent non-users because of ease of use, and a nonhormonal, prefertilization mechanism ofaction. This thesis details the expression in E. coliand characterization by transmissionelectron microscopy, of a potential anti-sperm vaccine consisting of the self-assembling ma-jor capsid protein of Human Papillomavirus, L1, chimerically decorated with extracellularfragments of the sperm specific calcium channel, CatSper, which is necessary for fertility. L1is well suited to be a scaffold for vaccine design because of the size, structure and stabilityof the particles it spontaneously forms, This is the first use of L1 as a scaffold for the pre-sentation of non-viral antigens, and may help pave the way for a broader adoption of L1 asa standardized scaffold for vaccine design.
dc.language.isoENG
dc.publisherRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofRensselaer Theses and Dissertations Online Collection
dc.subjectBiology
dc.titleProduction and characterization of chimeric human papillomavirus l1 virus-like particles for use as a contraceptive vaccine
dc.typeElectronic thesis
dc.typeThesis
dc.digitool.pid180402
dc.digitool.pid180404
dc.digitool.pid180405
dc.rights.holderThis electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.
dc.description.degreePhD
dc.relation.departmentDept. of Biological Sciences


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