[[The]] negative arm of the circadian clock imparts post-transcriptional regulation in neurospora crassa
Authors
Mosier, Alexander Eric
ORCID
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Other Contributors
Hurley, Jennifer M.
Makhatadze, George I.
Colón, Wilfredo
Forth, Scott T.
Makhatadze, George I.
Colón, Wilfredo
Forth, Scott T.
Issue Date
2021-05
Keywords
Biology
Degree
PhD
Terms of Use
This electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.
Full Citation
Abstract
We examined the intrinsically disordered nature of FRQ to establish conformational variability over the circadian day and found distinct temporal difference in protease susceptibility Next tested for changes in the FRQ interactome and found that FRQ interactors were variable and involved in diverse biological processes, further supporting the “hub” theory of regulation. Next, we analyzed these interactors for levels of protein disorder and found proteins with higher levels of disorder to more commonly bind at specific times of the day, leading to a potential regulatory pathway based on protein stabilization.. Finally, we examined one FRQ binding partner, CDC28, for post-transcriptional regulation and found that FRQ is both required for CDC28 phosphorylation and that there is circadian rhythmicity to this post-translational modification.
Description
May 2021
School of Science
School of Science
Department
Dept. of Biological Sciences
Publisher
Rensselaer Polytechnic Institute, Troy, NY
Relationships
Rensselaer Theses and Dissertations Online Collection
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