Browsing RPI Theses Open Access by Author "McGown, Linda Baine"
Now showing items 1-8 of 8
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A genome-wide approach to aptamer discovery
Fong, Casey H. (Rensselaer Polytechnic Institute, Troy, NY, 2020-05)Aptamers are short, single-stranded DNA or RNA oligonucleotides that bind with high specificity and affinity to a target molecule due to their unique, sequence-dependent secondary structure. They have found applications ... -
Astrobiological implications of guanosine gels and analysis of abiotic RNA polymerization
Cassidy, Lauren (Rensselaer Polytechnic Institute, Troy, NY, 2014-05)A robust analytical procedure is required in order to study model systems for abiotic RNA polymerization. Matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) has been used as a ... -
Characterization of guanosine based hydrogels and application of hydrogels in microchip electrophoresis for sequence based separation of ssDNA
Stevens, Wyatt Palmer (Rensselaer Polytechnic Institute, Troy, NY, 2019-05)Information about the bulk properties of the binary G-gels led to consideration of possible applications for them. Previous work in the McGown group had found that sequence-based separation of single stranded DNA was ... -
Characterization of two novel thermoresponsive mediums for capillary gel electrophoresis
Lemley, Corey S. (Rensselaer Polytechnic Institute, Troy, NY, 2011-12) -
Expanding the capabilities and utilities of flowing atmospheric pressure afterglow (FAPA) ionization source for mass spectrometry
Badal, Sunil Prasad (Rensselaer Polytechnic Institute, Troy, NY, 2018-12)Finally, issues with reproducible sample introduction and poor spatial resolution were addressed by coupling FAPA with laser-ablation (LA) sampling. Aerosolized particles from the ablation event were carried to the FAPA ... -
Mass spectrometry analysis of glycosaminoglycans and applications in biological research
Han, Xiaorui (Rensselaer Polytechnic Institute, Troy, NY, 2020-05)The glycosaminoglycan (GAG) family of polysaccharides includes the unsulfated hyaluronan and sulfated heparin, heparan sulfate, keratan sulfate, and chondroitin/dermatan sulfate. GAGs are involved in various biological ... -
Nucleolin-G-quadruplex interactions, with an emphasis on genome derived sequences
Fong, Casey (Rensselaer Polytechnic Institute, Troy, NY, 2017-05)The discovery of new DNA aptamers is an arduous process. The primary approach is the Systematic Evolution of Ligands by Exponential Enrichment (SELEX), where many different DNA sequences are screened against a specific ... -
Sequence-based separation of single-stranded DNA in capillary zone electrophoresis
Zhao, Jia (Rensselaer Polytechnic Institute, Troy, NY, 2018-08)This thesis explores further the mechanism for the sequence-based separation. First, the fluorescence tag attached to DNA was investigated as the cause of the sequenced-based separation. The same migration order was observed ...