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  • Characterization of Bacteroides Species from Human Intestine that Degrades Glycosaminoglycans 

    Ahn, M.Y.; Shin, K.H.; Kim, D.H.; Jung, E.A.; Toida, T.; Linhardt, Robert J.; Kim, Y.S. (NRC CNRC, 1998)
    Polysaccharide lyases that can degrade glycosaminoglycans (GAGs) were identified in an anaerobic strain living in the human intestine. The strain was isolated from the stool of a healthy male and identified as Bacteroides ...
  • Purification and Characterization of Heparin Lyases from Flavobacterium heparinum 

    Lohse, D.L.; Linhardt, Robert J. (Elsevier, 1992)
    Heparin lyase I has been purified from Flavobacterium heparinum and has been partially characterized (Yang, V. C., Linhardt, R. J., Berstein, H., Cooney, C. L., and Langer, R. (1985) J. Biol. Chem. 260, 1849-1857). There ...
  • Blood Deheparinization Using Immobilized Microbial Heparinase 

    Linhardt, Robert J.; Langer, R.; Cooney, C.L.; Galliher, P.M.; Flanagan, M.M.; Hoffberg, S.M. (Canadian Society for Chemical Engineering, 1981)
    Extracorporeal medical machines (e.g., artificial kidney, pump-oxygenator) perfused with blood have been an effective part of the therapeutic armamentarium for many years. These devices all rely on systemic heparinization ...
  • Characterization of heparin and severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) spike glycoprotein binding interactions 

    Kim, So Young; Jin, Weihua; Sood, Amika; Montgomery, David W.; Grant, Oliver C.; Fuster, Mark M.; Fu, Li; Dordick, Jonathan S.; Woods, Robert J.; Zhang, Fuming; Linhardt, Robert J. (Elsevier, 2020)
    Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has resulted in a pandemic and continues to spread around the globe at an unprecedented rate. To date, no effective therapeutic is available to fight its ...
  • Amphiphilic mPEG-modified oligo-phenylalanine nanoparticles chemoenzymatically synthesized via papain 

    Wang, Feng; Li, Youhua; Yu, Lu; Zhu, Jinwen; Zhang, Fuming; Linhardt, Robert J. (American Chemical Society (ACS), 2020-11-24)
    Amphiphilic mPEG-modified peptide nanoparticles were developed from oligo-phenylalanine (OPhe) nanoparticles (NPs) synthesized via papain. Tyndall effects indicate that OPhe NPs are amphiphobic. Addition of protein ...
  • The effect of electrospun scaffolds on the glycosaminoglycan profile of differentiating neural stem cells 

    Garrudo, F.F.F.; Mikael, P.E.; Xia, K.; Silva, J.C.; Ouyang, Y.; Chapman, C.A.; Hoffman, P.R.; Yu, Y.; Han, X.; Rodrigues, C.A.V.; Cabral, J.M.S.; Morgado, J.; Ferreir F.C.; Linhardt, R. J. (Elsevier, 2021-03-01)
    The use of electrospun scaffolds for neural tissue engineering applications allows a closer mimicry of the native tissue extracellular matrix (ECM), important for the transplantation of cells in vivo. Moreover, the role ...
  • Advances in preparation and synthesis of heparin and related products 

    Baytas, Sultan N.; Linhardt, Robert J. (Elsevier, 2020-12-01)
    Heparin is a naturally occurring glycosaminoglycan from livestock, principally porcine intestine, and is clinically used as an anticoagulant drug. A limitation to heparin production is that it depends on a single animal ...
  • Synthesis of selected unnatural sugar nucleotides for biotechnological applications 

    Qiao, Meng; Li, Bingzhi; Ji, Yuan; Lin, Lei; Linhardt, Robert; Zhang, Xing (Taylor and Francis, 2021-01-01)
    Sugar nucleotides are the principal building blocks for the synthesis of most complex carbohydrates and are crucial intermediates in carbohydrate metabolism. Uridine diphosphate (UDP) monosaccharides are among the most ...
  • Analysis of the glycosaminoglycan chains of proteoglycans 

    Song, Yuefan; Zhang, Fuming; Linhardt, Robert J. (Sage, 2021-02-01)
    Glycosaminoglycans (GAGs) are heterogeneous, negatively charged, macromolecules that are found in animal tissues. Based on the form of component sugar, GAGs have been categorized into four different families: heparin/heparan ...
  • Detection of glycosaminoglycans by polyacrylamide gel electrophoresis and silver staining 

    Lariviere, Wells B.; Han, Xiaorui; Oshima, Kaori; McMurtry, Sarah A.; Linhardt, Robert J.; Schmidt, Eric P. (Jove, 2021-02-01)
    Sulfated glycosaminoglycans (GAGs) such as heparan sulfate (HS) and chondroitin sulfate (CS) are ubiquitous in living organisms and play a critical role in a variety of basic biological structures and processes. As polymers, ...
  • Characterization of Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures and the Cortex of Neonatal Rats 

    Zhang, Xiaolu; Hashimoto, Joel G.; Han, Xiaorui; Zhang, Fuming; Linhardt, Robert J.; Guizzetti, Marina (Springer, 2021-03-01)
    Astrocytes are major producers of the extracellular matrix (ECM), which is involved in the plasticity of the developing brain. In utero alcohol exposure alters neuronal plasticity. Glycosaminoglycans (GAGs) are a family ...
  • Bottom-up and top-down profiling of pentosan polysulfate 

    Lin, Lei; Yu, Yanlei; Zhang, Fuming; Xia, Ke; Zhang, Xing; Linhardt, Robert J. (Royal Society of Chemistry, 2019-08-21)
    Pentosan polysulfate (PPS) is a semi-synthetic glycosaminoglycan (GAG) mimetic. PPS, synthesized through the chemical sulfonation of a plant-derived β-(1 → 4)-xylan, is the active pharmaceutical ingredient of the drug ...
  • Online capillary zone electrophoresis negative electron transfer dissociation tandem mass spectrometry of glycosaminoglycan mixtures 

    Stickney, Morgan; Sanderson, Patience; Leach, Franklin E.; Zhang, Fuming; Linhardt, Robert J.; Amster, I. Jonathan (Elsevier, 2019-11-01)
    Glycosaminoglycans (GAGs) are important biological molecules that are highly anionic and occur in nature as complex mixtures. A platform that combines capillary zone electrophoresis (CZE) separations with mass spectrometry ...
  • Polybenzimidazole nanofibers for neural stem cell culture 

    Garrudo, F.F.F.; Udangawa, R.N.; Hoffman, P.R.; Sordini, L.; Chapman, C.A.; Mikael, P.E.; Ferreira, F.A.; Silva, J.C.; Rodrigues, C.A.V.; Cabral, J.M.S.; Morgado, J.M.F.; Ferreira, F.C.; Linhardt, Robert J. (Elsevier, 2019-12-01)
    Neurodegenerative diseases compromise the quality of life of an increasing number of people in the world's aging population. While diagnosis is possible, no effective treatments are available. Strong efforts are needed to ...
  • Expedient synthesis of core disaccharide building blocks from natural polysaccharides for heparan sulfate oligosaccharide assembly 

    Pawar, Nitin J.; Wang, Lei; Higo, Takuya; Bhattacharya, Chandrabali; Kancharla, Pavan K.; Zhang, Fuming; Baryal, Kedar; Huo, Chang Xin; Liu, Jian; Linhardt, Robert J.; Huang, Xuefei; Hsieh-Wilson, Linda C. (Wiley, 2019-12-16)
    The complex sulfation motifs of heparan sulfate glycosaminoglycans (HS GAGs) play critical roles in many important biological processes. However, an understanding of their specific functions has been hampered by an inability ...
  • 3-O-sulfation of Heparan Sulfate Enhances Tau Interaction and Cellular Uptake 

    Zhao, Jing; Zhu, Yanan; Song, Xuehong; Xiao, Yuanyuan; Su, Guowei; Liu, Xinyue; Wang, Zhangjie; Xu, Yongmei; Liu, Jian; Eliezer, David; Ramlall, Trudy F.; Lippens, Guy; Gibson, James; Zhang, Fuming; Linhardt, Robert J.; Wang, Lianchun; Wang, Chunyu (Wiley, 2020-01-27)
    Prion-like transcellular spreading of tau in Alzheimer's Disease (AD) is mediated by tau binding to cell surface heparan sulfate (HS). However, the structural determinants for tau-HS interaction are not well understood. ...
  • Reconsidering conventional and innovative methods for pectin extraction from fruit and vegetable waste: Targeting rhamnogalacturonan I 

    Mao, Guizhu; Wu, Dongmei; Wei, Chaoyang; Tao, Wenyang; Ye, Xingqian; Linhardt, Robert J.; Orfila, Caroline; Chen, Shiguo (Elsevier, 2019-12-01)
    Background: Rhamnogalacturonan I (RG-I) is composed of a backbone of repeating disaccharide units →2)-α-L-Rhap-(1 → 4)-α-D-GalpA-(1→ with neutral sugar sidechains consisting of arabinose and galactose with variable linking ...
  • Functional role of glycosaminoglycans in decellularized lung extracellular matrix 

    Uhl, Franziska E.; Zhang, Fuming; Pouliot, Robert A.; Uriarte, Juan J.; Rolandsson Enes, Sara; Han, Xiaorui; Ouyang, Yilan; Xia, Ke; Westergren-Thorsson, Gunilla; Malmström, Anders; Hallgren, Oskar; Linhardt, Robert J.; Weiss, Daniel J. (Elsevier, 2020-01-15)
    Despite progress in use of decellularized lung scaffolds in ex vivo lung bioengineering schemes, including use of gels and other materials derived from the scaffolds, the detailed composition and functional role of ...
  • Characterization of heparin’s conformational ensemble by molecular dynamics simulations and nuclear magnetic resonance spectroscopy 

    Janke, J.J.; Yu, Y.; Pomin, Vitor H.; Zhao, J.; Wang, C.; Linhardt, Robert J.; García, A.E. (American Chemical Society (ACS), 2022-03-08)
    Heparin is a highly charged, polysulfated polysaccharide and serves as an anticoagulant. Heparin binds to multiple proteins throughout the body, suggesting a large range of potential therapeutic applications. Although its ...
  • Non-anticoagulant heparin as a pre-exposure prophylaxis prevents Lyme disease infection 

    Lin, Yi Pin; Yu, Yanlei; Marcinkiewicz, Ashley L.; Lederman, Patricia; Hart, Thomas M.; Zhang, Fuming; Linhardt, Robert J. (American Chemical Society (ACS), 2020-03-13)
    Lyme disease (LD) is caused by the spirochete Borrelia burgdorferi sensu lato (Bbsl). After transmission to humans by ticks, Bbsl spreads to multiple organs, leading to arthritis, carditis, and neuroborreliosis. No effective ...

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