Browsing The Linhardt Research Labs by Title
Now showing items 594-613 of 1022
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LC/MS Sequencing of Highly Sulfated Heparin-Derived Oligosaccharides
(Elsevier, 2004)Liquid chromatography/mass spectrometry (LC/MS) is applied to the analysis of complex mixtures of oligosaccharides obtained through the controlled, heparinase-catalyzed depolymerization of heparin. Reversed-phase ion-pairing ... -
Lectin Affinity Electrophoresis
(1995)Lectin affinity electrophoresis is a powerful technique to investigate the interaction between a lectin and its ligand. Affinity electrophoresis results from the reduced mobility of a charged species owing to its interaction ... -
Lectin Affinity Electrophoresis for the Separation of Fluorescently Labeled Sugar Derivatives
(1992-01-01)Lectin affinity electrophoresis was applied to the separation of charged, fluorescent conjugates of disaccharides. Four fluorescent conjugates were prepared by reductive amination of α-d-Man-(1→3)-d-Man, α-d-Gal-(1→4)-d-Gal, ... -
Lessons learned from the contamination of heparin
(Royal Society of Chemistry, 2009-03-09)Heparin is unique as one of the oldest drugs currently still in widespread clinical use as an anticoagulant, a natural product, one of the first biopolymeric drugs, and one of the few carbohydrate drugs. Recently, certain ... -
Lipase Catalyzed Synthesis and Characterization of Poly (Glycerol-Sebacate)
(2022)This study demonstrated that immobilized Candida antarctica lipase B (N435) catalysis in bulk leads to higher molecular weight poly(glycerol sebacate), PGS, than self-catalyzed condensation polymerization. Since the ... -
Lipase-Catalyzed Poly(glycerol-1,8-octanediol-sebacate): Biomaterial Engineering by Combining Compositional and Crosslinking Variables
(2022)This study examined poly(glycerol-1,8-octanediol-sebacate) (PGOS) copolymers with low-level substitution of O (1,8-octanediol) for G (glycerol) units (G/O ratios 0.5:0.5, 0.66:0.33, 0.75:0.25, 0.8:0.2, and 0.91:0.09) ... -
Liquid Chromatography-Mass Spectrometry to Study Chondroitin Lyase Action Pattern
(2009-02-01)Liquid chromatography–mass spectrometry was applied to determine the action pattern of different chondroitin lyases. Two commercial enzymes, chondroitinase ABC (Proteus vulgaris) and chondroitinase ACII (Arthrobacter ... -
Liquid to liquid extraction and liquid chromatography-tandem mass spectrometry determination of hainanmycin in feed
(2017-03-01)Hainanmycin is a new veterinary polyether antibiotic and has few sensitive analytical method in present days. In this study, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) relying on multiple reaction monitoring ... -
Localization and Characterization of Acharan Sulfate in the Body of the Giant African Snail Achatina fulica
(2001-01-01)Acharan sulfate is a glycosaminoglycan (GAG), having the structure -->4)-2-acetamido-2-deoxy-alpha-D-glucopyranose(1-->4)-2-sulfo-alpha-L-idopyranosyluronic acid (1-->, isolated from the body of the giant African snail ... -
Long Duration of Anticoagulant Activity and Protective Effects of Acharan Sulfate in Vivo
(2004-04-26)Introduction: We previously reported that a new glycosaminoglycan, acharan sulfate (AS) from the African giant snail Achatina fulica showed anticoagulant activity in vitro, but was much less active when compared to heparin. ... -
Loss and rescue of osteocalcin and osteopontin modulate osteogenic and angiogenic features of mesenchymal stem/stromal cells
(2020-10-01)Noncollagenous proteins in the bone extracellular matrix, such as osteocalcin (OC) and osteopontin (OPN), inherent to evolution of bone as a skeletal tissue, are known to regulate bone formation and mineralization. However, ... -
Loss of endothelial sulfatase-1 after experimental sepsis attenuates subsequent pulmonary inflammatory responses
(2019-01-01)Sepsis patients are at increased risk for hospital-acquired pulmonary infections, potentially due to postseptic immunosuppression known as the compensatory anti-inflammatory response syndrome (CARS). CARS has been attributed ... -
Loss of Hs3st3a1 or Hs3st3b1 enzymes alters heparan sulfate to reduce epithelial morphogenesis and adult salivary gland function
(Elsevier, 2021-09-01)Heparan sulfate 3-O-sulfotransferases generate highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes on cell surfaces and in the extracellular matrix. Previous ex vivo experiments suggested functional redundancy ... -
Low Molecular Weight Dermatan Sulfate as an Antithrombotic Agent: Structure Activity Relationship Studies
(1994-03-29)A structure-activity relationship of low molecular weight dermatan sulfate was undertaken to understand better this new non-heparin, glycosaminoglycan-based antithrombotic agent. A dermatan sulfate prepared from bovine ... -
Lysostaphin-functionalized cellulose fibers with antistaphylococcal activity for wound healing applications
(2011-12-01)With the emergence of "super bacteria" that are resistant to antibiotics, e.g., methicillin-resistant Staphylococcus aureus, novel antimicrobial therapies are needed to prevent associated hospitalizations and deaths. ... -
Macromolecular properties and hypolipidemic effects of four sulfated polysaccharides from sea cucumbers
(2017-10-01)The present study investigates the relationship between the high-order structure and hypolipidemic activity of four well-defined sulfated fucans from sea cucumber. The chain conformation, determined by a combination of AFM ... -
Major differences between the self-assembly and seeding behavior of heparin-induced- and in-vitro-phosphorylated tau and their modulation by potential inhibitors
(American Chemical Society (ACS), 2019-06-21)Self-assembly of the microtubule-associated protein tau into neurotoxic oligomers, fibrils, and paired helical filaments, and cell-to-cell spreading of these pathological tau species are critical processes underlying the ... -
MALDI-TOFMS Analysis of Hyaluronan Oligosaccharides
(Elsevier, 2007) -
MAPK/HOG signaling pathway induced stress-responsive damage repair a mechanism for Pichia pastoris to survive from hyperosmotic stress
(2021-02-01)Abstract BACKGROUND MAPK/HOG signaling pathway plays a key role in the response of yeast to external hyperosmotic stress. Over the past few decades, the regulation mechanism for this pathway in the robust yeast, Saccharomyces ... -
Mapping and Quantification of the Major Oligosaccharide Components of Heparin
(1988-01-01)A new method of determining the oligosaccharide composition of commercial glycosaminoglycan heparin is described in which heparin was first depolymerized using heparin lyase (EC 4.2.2.7), and then analysed by a single ...