Browsing The Linhardt Research Labs by Title
Now showing items 787-806 of 1022
-
Qualitative and quantitative analysis of branches in dextran using high-performance anion exchange chromatography coupled to quadrupole time-of-flight mass spectrometry
(2015-12-04)Dextran, a family of natural polysaccharides, consists of an α (1→6) linked-glucose main (backbone) chain having a number of branches. The determination of the types and the quantities of branches in dextran is important ... -
Qualitative and quantitative analysis of heparin and low molecular weight heparins using size exclusion chromatography with multiple angle laser scattering/refractive index and inductively coupled plasma/mass spectrometry detectors
(2017-11-03)Heparin, a highly sulfated glycosaminoglycan, has been used as a clinical anticoagulant over 80 years. Low molecular weight heparins (LMWHs), heparins partially depolymerized using different processes, are widely used as ... -
Qualitative and Quantitative Determination of Ten Major Saponins in Platycodi Radix by High Performance Liquid Chromatography with Evaporative Light Scattering Detection and Mass Spectrometry
(Elsevier, 2006-11-24)Saponins in Platycodi Radix (platycosides) exhibit potent biological activities in mammalian systems, including several beneficial effects such as anti-inflammatory, immunomodulatory and anti-obesity activities. In this ... -
Quantitation of heparosan with heparin lyase III and spectrophotometry
(2014-02-15)Heparosan is Escherichia coli K5 capsule polysaccharide, which is the key precursor for preparing bioengineered heparin. A rapid and effective quantitative method for detecting heparosan is important in the large-scale ... -
Quantitative Analysis of Chondroitin Sulfate in Raw Materials, Ophthalmic Solutions, Soft Capsules and Liquid Preparations
(2005-04-25)We performed the quantitative analysis of chondroitin sulfate (CS) obtained from raw materials and various pharmaceutical preparations. To quantify CS content in raw materials and in an ophthalmic solution, each test sample ... -
Quantitative analysis of heparan sulfate using isotopically labeled calibrants
(2020)Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions. Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount ... -
Quantitative analysis of the major linkage region tetrasaccharides in heparin
(2017-02-10)Heparin is a polysaccharide based anticoagulant drug composed of a complex mixture of glycosaminoglycan chains and peptidoglycosaminoglycan chains. In an effort to better characterize this important polysaccharide based ... -
Quantitative capillary electrophoresis determination of oversulfated chondroitin sulfate as a contaminant in heparin preparations
(Elsevier, 2009-05-01)A simple, accurate, and robust quantitative capillary electrophoresis (CE) method for the determination of oversulfated chondroitin sulfate (OSCS) as a contaminant in heparin (Hep) preparations is described. After degradation ... -
Quantitative Continuous Assay for Hyaluronan Synthase
(Elsevier, 2007-02-15)A rapid, continuous, and convenient three-enzyme coupled UV absorption assay was developed to quantitate the glucuronic acid and N-acetylglucosamine transferase activities of hyaluronan synthase from Pasteurella multocida ... -
Quantitification of Heparan Sulfate and Heparin Disaccharides Using Ion-pairing, Reverse-Phase, Micro-flow, High Performance Liquid Chromatography Coupled with Electrospray Ionization Trap Mass Spectrometry
(American Chemical Society (ACS), 2009)The glycosaminoglycan (GAG) family of biomacromolecules is composed acidic and linear chains of repeating disaccharide units. Quantitative disaccharide composition analysis is essential for the study and characterization ... -
Randomness in the Heparin Polymer: Computer Simulations of Alternative Action Patterns of Heparin Lyase
(1990-01-01)Heparin is a mixture of linear polysaccharides of undetermined sequence. Both biosynthetic data and computer simulation studies have established that each heparin polymer chain is comprised of oligosaccharides of defined ... -
Rapid and accurate determination of the lignin content of lignocellulosic biomass by solid-state NMR
(Elsevier, 2015-02-01)Biofuels and biomaterials, produced from lignocellulosic feedstock, require facile access to cellulose and hemicellulose to be competitive with petroleum processing and sugar-based fermentation. Physical-chemical barriers ... -
Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in E. coli
(Oxford University Press, 2016)Robust gene circuit construction requires use of promoters exhibiting low crosstalk. Orthogonal promoters have been engineered utilizing an assortment of natural and synthetic transcription factors, but design of large ... -
Rational identification and characterisation of peptide ligands for targeting polysialic acid
(Nature, 2020-12-01)Rational identification and characterisation of peptide ligands for targeting polysialic acid -
Recent advances in biotechnology for heparin and heparan sulfate analysis
(Elsevier, 2020-11-01)Heparan sulfate (HS) is a class of linear, sulfated, anionic polysaccharides, called glycosaminoglycans (GAGs), which present on the mammalian cell surfaces and extracellular matrix. HS GAGs display a wide range of critical ... -
Recent advances in sulfotransferase enzyme activity assays
(2012-06-01)Sulfotransferases are enzymes that catalyze the transfer of sulfo groups from a donor, for example 3′-phosphoadenosine 5′-phosphosulfate, to an acceptor, for example the amino or hydroxyl groups of a small molecule, ... -
Recent Advances in the Synthesis of C-oligosaccharides
(Bentham Science, 2005-12-01)This paper reviews the recent advances in the synthesis of catabolically stable sugar mimetics, C-oligosaccharides. These compounds are synthetic analogs of the naturally occurring O-oligosaccharides, in which the ... -
Recent advances on the one-pot synthesis to assemble size-controlled glycans and glycoconjugates and polysaccharides
(2021-04-15)Glycans and glycoconjugates in nature include macromolecules with important biological activities and widely distributed in all living organisms. These oligosaccharides and polysaccharides play important roles in a variety ... -
Recent Chemical and Enzymatic Approaches to the Synthesis of Glycosaminoglycan Oligosaccharides
(2003-09-29)Glycosaminoglycans, highly charged polycarboxylated, polysulfated polysaccharides, are an important class of therapeutic agents and investigational drug candidates. Heparin has been widely used as a clinical anticoagulant ... -
Recent Progress and Advanced Technology in Carbohydrate-Based Drug Development
(2021-06-01)Carbohydrates, one of the most abundant and widespread biomolecules in nature, play indispensable roles in diverse biological functions, and represent a treasure trove of untapped potential for pharmaceutical applications. ...