Browsing The Linhardt Research Labs by Title
Now showing items 971-990 of 1022
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Tandem MS Can Distinguish Hyaluronic Acid from N-Acetylheparosan
(2008-01-01)Isobaric oligosaccharides enzymatically prepared from hyaluronic acid (HA) and N-acetylheparosan (NAH), were distinguished using tandem mass spectrometry. The only difference between the two series of oligosaccharides was ... -
Targeted delivery of phycocyanin for prevention of colon cancer using electrospun fibers
(2019-04-01)Phycocyanin (PC), a water-soluble biliprotein, exhibits potent anti-colon cancer properties. However, its application in functional foods is limited by the poor stability and low bioavailability of PC. In this study, we ... -
Tetrahedron Report Number 462 - Recent Advances in StereoselectiveC-Glycoside Synthesis
(1998)Carbohydrate analogs in which a carbon atom substitutes for the glycosidic oxygen are called Cglycosides. During the last two decades, the synthesis of C-glycosides has become an area of intense study among carbohydrate ... -
The abnormal accumulation 1 of heparan sulfate in patients with mucopolysaccharidosis prevents the elastolytic activity of cathepsin V
(2020)Mucopolysaccharidosis (MPS) are rare inherited diseases characterized by accumulation of lysosomal glycosaminoglycans, including heparan sulfate (HS). Patients exhibit progressive multi-visceral dysfunction and shortened ... -
The Effect of Dissolved Oxygen (DO) Concentration on the Glycosylation of Recombinant Protein Produced by the Insect Cell-Baculovirus System
(2002)The effect of dissolved oxygen concentration on human secreted alkaline phosphatase (SEAP) glycosylation by the insect cell-baculovirus expression system was investigated in a well-controlled bioreactor. Oligomannose-type ... -
The effect of electrospun scaffolds on the glycosaminoglycan profile of differentiating neural stem cells
(Elsevier, 2021-03-01)The use of electrospun scaffolds for neural tissue engineering applications allows a closer mimicry of the native tissue extracellular matrix (ECM), important for the transplantation of cells in vivo. Moreover, the role ... -
The Effect of Extracorporeal Enzymatic Deheparinization on the Formed Blood Components
(1984-01-01)A stirred blood filter containing an immobilized enzyme, heparinase, has been used to neutralize heparin's anticoagulant activity at the outflow of an extracorporeal circuit in dogs. The hematocrit and red blood cell count ... -
The Effects of Culture Conditions on the Glycosylation of Secreted Human Placental Alkaline Phosphatase Produced in Chinese Hamster Ovary Cells
(Wiley, 2008-08-15)The effects of different culture conditions, suspension and microcarrier culture and temperature reduction on the structures of N-linked glycans attached to secreted human placental alkaline phosphatase (SEAP) were ... -
The effects of metal ions on heparin/heparin sulfate-protein interactions
(ELYNS, 2014)Heparin/heparin sulfate (HS) interacts with a number of proteins thereby playing an essential role in the regulation of many physiological processes. The understanding of heparin/HS-protein interactions at the molecular ... -
The First Total Synthesis of Calabricoside A
(2003-09-01)Quercetin 3-O-[α-l-rhamnopyranosyl-(1→2)-α-l-arabinopyranoside]-7-O-β-d-glucopyranoside (calabricoside A), a new flavonol triglycoside isolated from the aerial parts of Putoria calabrica showing strong radical scavenging ... -
The First Total Synthesis of Sporiolide A
(American Chemical Society (ACS), 2006-10-27)The first total synthesis of the natural cytotoxic agent sporiolide A has been accomplished from D-glucal in 16 steps with 6.1% overall yield. Carbohydrates were applied as the chiral templates to manipulate the absolute ... -
The proteoglycan bikunin has a defined sequence
(Nature, 2011-01-01)Proteoglycans are complex glycoconjugates that regulate critical biological pathways in all higher organisms. Bikunin, the simplest proteoglycan, with a single glycosaminoglycan chain, is a serine protease inhibitor used ... -
The Regulation of Heparinase Production in Flavobacterium heparinum
(1982)The effect of various carbon, nitrogen and sulfur sources on the production of heparinase by Flavobacterium heparinum in defined medium in the presence and absence of heparin as the inducer has been studied. Carbon catabolite ... -
The Road to Animal-Free Glycosaminoglycan Production: Current Efforts and Bottlenecks
(2018-10-01)Animal-extraction, despite its limitations, continues to monopolize the fast-growing glycosaminoglycan (GAG) industry. The past few years have seen an increased interest in the development of alternative GAG production ... -
The Role of the Methoxyphenol Apocynin, a Vascular NADPH Oxidase Inhibitor, as a Chemopreventative Agent in the Potential Treatment of Cardiovascular Diseases
(2008-12-01)Oxidative stress has been linked to the origin and progression of cardiovascular diseases. Nicotinamide adenine dinucleotide phosphate, reduced form (NADPH) oxidase is a multi-component, NADPH-dependent enzyme that generates ... -
The Stereospecific Synthesis of KDN a-C-glycosides by Samarium-Mediated Reductive Desulfonylation ofGlycosyl Phenylsulfone
(1998)KDN, 3-deoxy-D-glycero-D-galacto-2-nonulopyranosylonic acid, is a novel type of sialic acid in which the acetamido group at C-S of N-acetylneuraminic acid is replaced by a hydroxyl group. This ulosonic acid was first ... -
The structure-activity relationship of the interactions of SARS-CoV-2 spike glycoproteins with glucuronomannan and sulfated galactofucan from Saccharina japonica
(2020-11-15)The SARS-CoV-2 spike glycoproteins (SGPs) and human angiotensin converting enzyme 2 (ACE2) are the two key targets for the prevention and treatment of COVID-19. Host cell surface heparan sulfate (HS) is believed to interact ... -
The US regulatory and pharmacopeia response to the global heparin contamination crisis
(Nature, 2016-06-09)The contamination of the widely used lifesaving anticoagulant drug heparin in 2007 has drawn renewed attention to the challenges that are associated with the characterization, quality control and standardization of complex ... -
Thermodynamic Analysis of Heparin Interaction with a Basic CyclicProtein Using Isothermal Titration Calorimetry
(1998)Brain natriuretic peptide (BNP) was examined as part of a continuing study of the interaction of proteins and peptides with the glycosaminoglycan heparin. BNP was tentatively identified as a heparin-binding protein on the ... -
Thin Layer Chromatography for the Analysis of Glycosaminoglycan Oligosaccharides
(Elsevier, 2007-12-01)Thin layer chromatography was used to analyze glycosaminoglycan oligosaccharides obtained through the use of polysaccharide lyases. This method allows for the rapid, semi-quantitative analysis of a wide variety of ...