Browsing The Linhardt Research Labs by Title
Now showing items 27-46 of 1022
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A System for Heparin Removal
(1982)Extracorporeal medical machines rely on systemic heparinization to improve blood compatibility. However, heparin can lead to serious complications such as hemorrhaging. We propose a new approach to control heparin levels ... -
Abnormally high content of free glucosamine residues identified in a preparation of commercially available porcine intestinal heparan sulfate
(American Chemical Society (ACS), 2016-07-05)Heparan sulfate (HS) polysaccharides are ubiquitous in animal tissues as components of proteoglycans, and they participate in many important biological processes. HS carbohydrate chains are complex and can contain rare ... -
Accelerated Degradation of Poly(e-caprolactone) by Organic Amines
(1994)The solid-state degradation of poly(epsilon-caprolactone) catalyzed by primary, secondary and tertiary alkylamines was investigated. The degradation process was monitored by weight loss and molecular weight change measured ... -
Accelerated Heparin Stability Studies
(1996-01-01)The objective of this study was to extend our understanding of the stability of heparin. Sodium heparin, derived from porcine intestinal mucosa, was first incubated in 0.1 N hydrochloric acid and 0.1 N sodium hydroxide at ... -
Acceptor Specificity of the Pasteurella Hyaluronan and Chondroitin Synthases and Production of Chimeric Glycosaminoglycans
(Elsevier, 2007-01-05)The hyaluronan (HA) synthase, PmHAS, and the chondroitin synthase, PmCS, from the Gram-negative bacterium Pasteurella multocida polymerize the glycosaminoglycan (GAG) sugar chains HA or chondroitin, respectively. The ... -
Action Pattern of Polysaccharide Lyases on Glycosaminoglycans
(1994-06-01)The action pattern of polysaccharide lyases on glycosaminoglycan substrates was examined using viscosimetric measurements and gradient polyacrylamide gel electrophoresis (PAGE). Heparin lyase I (heparinase, EC 4.2.2.7) and ... -
Active Site of Chondroitin AC Lyase Revealed by the Structure of Enzyme-Oligosaccharide Complexes and Mutagenesis
(2001-02-27)The crystal structures of Flavobacterium heparinium chondroitin AC lyase (chondroitinase AC; EC 4.2.2.5) bound to dermatan sulfate hexasaccharide (DShexa), tetrasaccharide (DStetra), and hyaluronic acid tetrasaccharide ... -
Additional role of nicotinic acid hydroxylase for the transformation of 3-succinoyl-pyridine by Pseudomonas sp. JY-Q
(2021-03-01)Nicotine and nicotinic acid (NA) are both considered to be representatives of N-heterocyclic aromatic compounds, and their degradation pathways have been revealed in Pseudomonas species. However, the cooccurrence of these ... -
Addressing endotoxin issues in bioengineered heparin
(2012-11-01)Heparin is a widely used clinical anticoagulant that is prepared from pig intestine. A contamination of heparin in 2008 has led to a reexamination of animal-derived pharmaceuticals. A bioengineered heparin prepared by ... -
Adsorption of doxorubicin on poly(methyl methacrylate)−chitosan−heparin-coated activated carbon beads
(2012)Extracorporeal filter cartridges, filled with an activated carbon bead (ACB) adsorbent, have been used for removal of overdosed cancer drugs from the blood. Coatings on adsorbent matrices, poly(methyl methacrylate) ... -
Advances in preparation and synthesis of heparin and related products
(Elsevier, 2020-12-01)Heparin is a naturally occurring glycosaminoglycan from livestock, principally porcine intestine, and is clinically used as an anticoagulant drug. A limitation to heparin production is that it depends on a single animal ... -
Affinity Capillary Electrophoresis Employing Immobilized Glycosaminoglycanto Resolve Heparin-Binding Peptides
(1998)A new capillary electrophoresis technique has been developed for the affinity resolution of synthetic heparin-binding peptides using an immobilized glycosaminoglycan. Heparin and heparan sulfate were immobilized onto fused ... -
Affinity Chromatography Using Enzymatically Synthesized Nucleotide-ContainingDNA Binding Polymers
(1999)A series of nucleotide-containing polyphenols has been synthesized by a simple, two-step enzymatic method. The binding properties of these synthetic polymers to complementary oligonucleotides have been evaluated using a ... -
Affinity Purification of Secreted Alkaline Phosphatase Produced by the Baculovirus Expression Vector System
(2001-01-01)Human secreted alkaline phosphatase (SEAP) was produced in a stably-transformed Spodoptera frugiperda Sf-9 insect cell line (Sfb4GalT) following infection with a recombinant Autographa californica multiple nuclear ... -
Affinity, kinetic and structural study of the interaction of 3-O-sulfotransferase isoform 1 with heparan sulfate
(American Chemical Society (ACS), 2006-04-25)The 3-O-sulfonation of glucosamine residues in heparan sulfate (HS) by 3-O-sulfotransferase (3-OST) is a key substitution that is present in HS sequences of biological importance, in particular HS anticoagulant activity. ... -
Akebia saponin D reverses corticosterone hypersecretion in an Alzheimer's disease rat model
(2018-11-01)Background: Glucocorticoid hormones are implicated in the pathogenesis of Alzheimer's disease (AD) and other diseases including diabetes, hyperlipidemia, and osteoporosis. Akebia saponin D (ASD) possesses numerous ... -
Alveolar heparan sulfate shedding impedes recovery from bleomycin-induced lung injury
(2020-06-01)The pulmonary epithelial glycocalyx, an anionic cell surface layer enriched in glycosaminoglycans such as heparan sulfate and chondroitin sulfate, contributes to the alveolar barrier. Direct injury to the pulmonary epithelium ... -
Amphiphilic bromelain-synthesized oligo-phenylalanine grafted with methoxypolyethylene glycol possessing stabilizing thermo-responsive emulsion properties
(2019-03-07)A thermo-responsive amphiphile was developed from oligo-phenylalanine [oligo(Phe)]. The hydrophobic moiety of the amphiphile, oligo(Phe) was synthesized via reverse hydrolysis catalyzed by bromelain in dimethyl sulfoxide ... -
Amphiphilic mPEG-modified oligo-phenylalanine nanoparticles chemoenzymatically synthesized via papain
(American Chemical Society (ACS), 2020-11-24)Amphiphilic mPEG-modified peptide nanoparticles were developed from oligo-phenylalanine (OPhe) nanoparticles (NPs) synthesized via papain. Tyndall effects indicate that OPhe NPs are amphiphobic. Addition of protein ...