Browsing The Linhardt Research Labs by Title
Now showing items 282-301 of 1022
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Digestibility of squash polysaccharide under simulated salivary, gastric and intestinal conditions and its impact on short chain fatty acid production in type-2 diabetic rats
(2020-05-01)In vitro digestive conditions were simulated to investigate the digestibility of polysaccharides prepared from squash (SPS). A small amount of free glucose monosaccharide was released after salivary and intestinal digestion ... -
Dimerization Interface of Osteoprotegerin Revealed by Hydrogen-deuterium Exchange Mass Spectrometry
(Elsevier, 2018-01-01)Previous structural studies of osteoprotegerin (OPG), a crucial negative regulator of bone remodeling and osteoclastogenesis, were mostly limited to the N-terminal ligand-binding domains. It is now known that the three ... -
Direct Measurement of the Interaction of Glycosaminoglycans and a Heparin Decasaccharide with Malaria Circumsporozoite Protein
(2001-09-25)Circumsporozoite (CS) protein is a predominant surface antigen of malaria sporozoites, the infective form of the parasite, and has been used for making anti-malaria vaccines. For the first time we have examined the interaction ... -
Directional Immobilization of Heparin onto the Nonporous Surface of Polystyrene Microplates
(Future Science, 1997)Heparin, a sulfated polysaccharide,exhibits anticoagulant activity mediat-ed through the binding of a specificpentasaccharide sequence in its struc-ture to the protein antithrombin III, acoagulation protease ... -
Directional Immobilization of Heparin to Beaded Supports
(1994-01-01)Heparin was immobilized in a defined orientation on Sepharose, agarose, and polyacrylamide supports by coupling through its reducing end. This is expected to mimic the attachment of heparin to the protein core in the ... -
Disaccharide analysis of glycosaminoglycan mixtures by ultra-performance liquid chromatography-mass spectrometry
(Elsevier, 2012)Glycosaminoglycans are a family of polysaccharides widely distributed in all eukaryotic cells. These polyanionic, linear chain polysaccharides are composed of repeating disaccharide units that are often differentially ... -
Disaccharide Compositional Analysis of Heparin and Heparan Sulfate Using Capillary Zone Electrophoresis
(1991-01-01)Capillary zone electrophoresis (CZE) was used to separate eight commercial disaccharide standards of the structure delta UA2X(1----4)-D-GlcNY6X (where delta UA is 4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid, GlcN ... -
Dissecting the substrate recognition of 3-O-sulfotransferase for the biosynthesis of anticoagulant heparin
(2012-04-03)Heparin is a polysaccharide-based natural product that is used clinically as an anticoagulant drug. Heparan sulfate 3-O-sulfotransferase (3-OST) is an enzyme that transfers a sulfo group to the 3-OH position of a glucosamine ... -
Distinct Heparan Sulfate Compositions in Wild-Type and Pipe-Mutant Eggshell Matrix
(Taylor and Francis, 2008-01-01)Spatial information embedded in the extracellular matrix establishes the dorsoventral polarity of the Drosophila embryo through the ventral activity of a serine protease cascade. Pipe is a Golgi-localized protein responsible ... -
Distinguishing Glucuronic from Iduronic Acid in Glycosaminoglycan Tetrasaccharides by Using Electron Detachment Dissociation
(American Chemical Society (ACS), 2007-03-05)Distinguishing the epimers iduronic acid (IdoA) and glucuronic acid (GlcA) has been a long-standing challenge for the mass spectrometry analysis of glycosaminoglycan (GAG) oligosaccharides. In this work, electron detachment ... -
Distribution of N-Acetylneuraminic Acid and Sialylglycan in Eggs in Silky Fowl
(2003-03-01)The distribution of sialic acid in the eggs of original Silky fowl was investigated. The sialic acid contents of the yolk, albumen and the chalaza of a single egg were 205·2, 11·96 and 0·83 mg, respectively. 2. The sialic ... -
Divergent effect of glycosaminoglycans on the in vitro aggregation of serum amyloid A
(2014-01-01)Serum amyloid A (SAA) is an apolipoprotein involved in poorly understood roles in inflammation. Upon trauma, hepatic expression of SAA rises 1000 times the basal levels. In the case of inflammatory diseases like rheumatoid ... -
Diversity Does Make a Difference: Fibroblast Growth Factor - HeparinInteractions
(1998)Fibroblast growth factors (FGFs) are members of a protein family with a broad range of biological activities. The best characterized FGFs interact with two distinct extracellular receptors — a transmembrane tyrosine kinase ... -
Domain structure elucidation of human decorin glycosaminoglycans
(Portland Press, 2010-10-15)The structure of the GAG (glycosaminoglycan) chain of recombinantly expressed decorin proteoglycan was examined using a combination of intact-chain analysis and domain compositional analysis. The GAG had a number-average ... -
Dose-dependent neuroprotective effect of oriental phyto-derived glycyrrhizin on experimental neuroterminal norepinephrine depletion in a rat brain model
(2019-08-01)The dose-dependent neuroprotective role of licorice-derived glycyrrhizin during subacute neuroterminal norepinephrine (NE) depletion was studied in rat brain. Experimental design included thirty 5-week-old male rats randomly ... -
Draft genome sequence of Bacillus subtilis Ia1a, a new strain for poly-γ-glutamic acid and exopolysaccharides production
(American Society for Microbiology, 2016-01-01)We report here the 4.092-Mb high-quality draft genome assembly of a newly isolated poly-γ-glutamic acid–producing strain, Bacillus subtilis Ia1a. The genome sequence is considered a critical tool to facilitate the engineering ... -
Draft Genome Sequence of Escherichia coli Strain ATCC #23502 (1 Serovar O5:K4(L):H4)
(The American Society for Microbiology (ASM), 2013)We report the 4.682-Mbp high-quality draft assembly of the Escherichia coli strain ATCC 23502 (serovar O5:K4:H4, also known as NCDC U1-41) genome. This uropathogenic strain, commonly referred to as E. coli K4, produces a ... -
Draft Genome Sequence of Escherichia coli Strain ATCC #23506 (Serovar 1 O10:K5(L):H4)
(The American Society for Microbiology (ASM), 2013)We report the 5.101-Mbp high-quality draft assembly of the Escherichia coli strain ATCC 23506 (serovar O10:K5:H4, also known as NCDC Bi 8337-41) genome. This uropathogenic strain, commonly referred to as E. coli K5, produces ... -
Draft Genome Sequence of Escherichia coli Strain Nissle 1917 1 (Serovar O6:K5:H1)
(The American Society for Microbiology (ASM), 2013)We announce the availability of the 5.023-Mbp high-quality draft assembly of the Escherichia coli strain Nissle 1917 (serovar O6:K5:H1) genome. Short genomic segments from this important probiotic strain have been available ... -
Dromedary glycosaminoglycans: Molecular Characterization of Camel Lung and Liver Heparan Sulfate
(Elsevier, 2006-01-01)Glycosaminoglycans (GAGs) are the portion of a proteoglycan that determine its final shape and function. The molecular structure of predominant GAG species in camel liver and lung is reported for the first time. The ...