Browsing The Linhardt Research Labs by Title
Now showing items 468-487 of 1022
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Heparin stability by determining unsubstituted amino groups using HILIC-MS
(Elsevier, 2014)The thermal instability of the anticoagulant heparin is associated, in part, with the solvolytic loss of N-sulfo groups. This study describes a new method to assess the increased content of unsubstituted amino groups present ... -
Heparin's Solution Structure Determined by Small-Angle Neutron Scattering (SANS)
(Wiley, 2016)Heparin is a linear, anionic polysaccharide that is widely used as a clinical anticoagulant. Despite its discovery 100 years ago in 1916, the solution structure of heparin remains unknown. The solution shape of heparin has ... -
Heparin-based nanoparticles
(2010)Numerous papers on heparin nanoparticles have been reported regarding targeting therapy and biomedical imaging. Here, we have summarized the prospects and opportunities of heparin as a carrier for cancer targeting and ... -
Heparin-Binding Growth-Associated Molecule Contains Two Heparin Binding b-Sheet Domains That Are Homologous to the Thrombospondin Type I Repeat
(Esevier, 2000)Heparin-binding growth-associated molecule (HB-GAM) is an extracellular matrix-associated protein implicated in the development and plasticity of neuronal connections of brain. Binding to cell surface heparan sulfate is ... -
Heparin-mediated dimerization of follistatin
(2021-02-01)Heparin and heparan sulfate (HS) are highly sulfated polysaccharides covalently bound to cell surface proteins, which directly interact with many extracellular proteins, including the transforming growth factor-β (TGFβ) ... -
Heparin-Protein Interactions
(2002)Heparin, a sulfated polysaccharide belonging to the family of glycosaminoglycans, has numerous important biological activities, associated with its interaction with diverse proteins. Heparin is widely used as an anticoagulant ... -
Heparin/heparan sulfate analysis by covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry
(Elsevier, 2018-04-13)Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures. Covalent modification ... -
Heparin: Past, Present, and Future
(Multidisciplinary Digital Publishing Institute (MDPI), 2016)Heparin, the most widely used anticoagulant drug in the world today, remains an animal-derived product with the attendant risks of adulteration and contamination. A contamination crisis in 2007-2008 increased the impetus ... -
Heparinase I Acts on a Synthetic Heparin Pentasaccharide Corresponding to the Antithrombin III Binding Site
(2000-12-15)A synthetic pentasaccharide, containing an intact antithrombin III (ATIII) binding site that is in clinical studies a specific antifactor Xa agent, serves as a substrate for a heparin lyase (heparinase I, EC 4.2.2.7) from ... -
Heparinase I selectivity for the 3,6-di-O-sulfo-2-deoxy-2-sulfamido-a-D-glucopyranose (1,4) 2-O-sulfo-a-L-idopyranosyluronic acid (GlcNS3S6S-IdoA2S) linkages
(2011)Porcine intestinal mucosa heparin was partially depolymerized by recombinant heparinase 1 (heparin lyase 1, originating from Flavobacterium heparinum and expressed in Escherichia coli) and then fractionated, leading to the ... -
Heparinase Production by Flavobacterium heparinum
(1981)Heparinase production by Flavobacterium heparinum in complex protein digest medium, with heparin employed as the inducer, has been studied and improved. The maximum productivity of heparinase has been increased 156-fold ... -
Heparinase: In Vivo Activity and Immunogenicity in Rabbits
(1983)Anticoagulation with heparin is required during extracorporeal circulation for hemodialysis and cardiopulmonary bypass as well as during vascular surgery. Reversal of anticoagulation with protamine may be associated with ... -
Heparinoids activate a tumor-secreted protease via tethering and V-type allostery
(American Chemical Society (ACS), 2014)The chemoenzymatic synthesis of heparan sulfate tetrasaccharide (1) and hexasaccharide (2) with a fluorous tag attached at the reducing end is reported. The fluorous tert-butyl dicarbonate (FBoc) tag did not interfere with ... -
Heparinoids: Structure, Biological Activities and Therapeutic Applications
(1999-06-08)Heparin is an important polyanionic drug having a wide variety of different biological activities. Substantial research effort has focused on the preparation of improved heparins and heparin analogues that might exhibit ... -
Heparosan Chain Characterization: Sequential Depolymerization of E. coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers,
(2021-03-01)Heparosan is a non-sulfated polysaccharide and potential applications include, chemoenzymatic synthesis of heparin and heparan sulfates. Heparosan is produced using microbial cells (natural producers or engineered cells). ... -
Hexuronic acid stereochemistry determination in chondroitin sulfate glycosaminoglycan oligosaccharides by electron detachment dissociation
(American Chemical Society (ACS), 2012-09-01)Electron detachment dissociation (EDD) has previously provided stereo-specific product ions that allow for the assignment of the acidic C-5stereochemistry in heparan sulfate glycosaminoglycans (GAGs), but application of ... -
High cell density cultivation of a recombinant E. coli strain expressing a key enzyme in bioengineered heparin production
(Springer, 2013-05-01)A bioengineered heparin, as a replacement for animal-derived heparin, is under development that relies on the fermentative production of heparosan by Escherichia coli K5 and its subsequent chemoenzymatic modification using ... -
High cell density cultivation of a recombinant Escherichia coli strain expressing a 6-O-sulfotransferase for the production of bioengineered heparin
(Wiley, 2015-01-01)Aims: One of six heparin biosynthetic enzymes, cloned and expressed in Escherichia coli as a soluble fusion protein, requires large-scale preparation for use in the chemoenzymatic synthesis of heparin, an important ... -
High cell density cultivation of recombinant Escherichia coli strains expressing 2-O-sulfotransferase and C5-epimerase for the production of bioengineering heparin
(2015)Bioengineered heparin is being investigated as a potential substitute for the animal-sourced anticoagulant drug. One step in the current process to prepare bioengineered heparin involves the conversion of N-sulfo heparosan, ... -
High Conductivity and High Capacitance Electrospun Fibers for Supercapacitor Applications
(2020-04-29)Electrospinning is a simple method for producing nanoscale or microscale fibers from a wide variety of materials. Intrinsically conductive polymers (ICPs), such as polyaniline (PANI), show higher conductivities with the ...