Browsing The Linhardt Research Labs by Title
Now showing items 490-509 of 1022
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High Resolution Crystal Structure of Arthrobacter aurescens Chondroitinase AC Complexed with Chondroitin Sulfate Defines the Catalytic Mechanism
(2004)Chondroitin lyases (EC 4.2.2.4 and EC 4.2.2.5) are glycosaminoglycan-degrading enzymes that act as eliminases. Chondroitin lyase AC from Arthrobacter aurescens (ArthroAC) is known to act on chondroitin 4-sulfate and ... -
High Sensitivity Detection of Active Botulinum Neurotoxin (BoNT) by Glyco-qPCR
(American Chemical Society (ACS), 2014)The sensitive detection of highly toxic botulinum neurotoxin (BoNT) from Clostridium botulinum is of critical importance because it causes human illnesses if foodborne or introduced in wounds and as an iatrogenic substance. ... -
High structural resolution hydroxyl radical protein footprinting reveals an extended Robo1-heparin binding interface
(2015-04-24)Interaction of transmembrane receptors of the Robo family and the secreted protein Slit provides important signals in the development of the central nervous system and regulation of axonal midline crossing. Heparan sulfate, ... -
High-field asymmetric-waveform ion mobility spectrometry and electron detachment dissociation of isobaric mixtures of glycosaminoglycans
(American Chemical Society (ACS), 2014-02-01)High-field asymmetric waveform ion mobility spectrometry (FAIMS) is shown to be capable of resolving isomeric and isobaric glycosaminoglycan negative ions and to have great utility for the analysis of this class of molecules ... -
High-Performance Liquid Chromatographic Separation of Heparin Derived Oligosaccharides
(1985-11-01)Heparin has been enzymatically depolymerized with heparinase (heparin lyase (EC 4.2.2.7)) and then separated into di-, tetra-, hexa-, octa-, and decasaccharide mixtures by low-pressure gel-permeation chromatography (GPC). ... -
High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis
(Elsevier, 2010-01-01)Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially ... -
High-throughput method for in process monitoring of 3-O-sulfotransferase catalyzed sulfonation in bioengineered heparin synthesis
(2019-12-01)Bioengineered heparin (BEH) offers a potential alternative for the preparation of a safer pharmacological heparin. Construction of in-process control assays for tracking each enzymatic step during bioengineered heparin ... -
Highly branched RG-I domain enrichment are indispensable for pectin mitigating against high-fat diet-induced obesity
(2020-08-12)Obesity is associated with gut microbiome dysbiosis. Our previous research has shown that highly branched rhamnogalacturonan type I (RG-I)-enriched pectin (WRP, 531.5 kDa, 70.44% RG-I, Rha/(Gal + Ara) = 20) and its ... -
Highly purified fucosylated chondroitin sulfate oligomers with selective intrinsic factor Xase complex inhibition
(2019-10-15)Fucosylated chondroitin sulfate (FCS) oligosaccharides of specific molecular weight have shown potent anticoagulant activities with selectivity towards intrinsic factor Xase complex. However, the preparation of FCS ... -
Highly Swelling Hydrogels from Ordered Galactose-Based Poly (Acrylate)s
(1998)High swelling galactose-based hydrogels have been prepared using a chemoenzymatic procedure. Regioselective acylation of beta-O-methyl-galactopyranoside in nearly anhydrous pyridine with lipase from Pseudomonas cepacia ... -
Homogalacturonan from squash: Characterization and tau-binding pattern of a sulfated derivative
(2022-06-01)A pectic polysaccharide (WAP) was isolated from squash and identified as a homogalacturonan with a molecular mass of 83.2 kDa by GPC, monosaccharide composition analysis, FT-IR and NMR spectra. Sulfation modification of ... -
Homogeneous, Structurally Defined Heparin-Oligosaccharides With Low Anticoagulant Activity Inhibit the Generation of the Amplification Pathway C3 Convertase In Vitro
(Elsevier, 1988)This paper demonstrates that heparin-oligosaccharides with low anticoagulant activity have a high capacity to inhibit activation of the amplification pathway of complement in vitro. We prepared heparin-oligosaccharides by ... -
Host and pathogen glycosaminoglycan-binding proteins modulate antimicrobial peptide responses
(2011-02-01)During group B streptococcal infection, the alpha C protein (ACP) on the bacterial surface binds to host cell surface heparan sulfate proteoglycans (HSPGs) and facilitates entry of bacteria into human epithelial cells. ... -
HSulf-1 modulates FGF-2 and hypoxia mediated migration and invasion of breast cancer cells
(American Association for Cancer Research (AACR), 2011)HSulf-1 modulates the sulfation states of heparan sulfate proteoglycans critical for heparin binding growth factor signaling. In the present study, we show that HSulf-1 is transcriptionally deregulated under hypoxia in ... -
Human Follicular Fluid Heparan Sulfate Contain Abundant 3-O-Sufated Chains with Anticoagulant Activity
(Elsevier, 2008)Anticoagulant heparan sulfate proteoglycans bind and activate antithrombin by virtue of a specific 3-O-sulfated pentasaccharide. They not only occur in the vascular wall but also in extravascular tissues, such as the ovary, ... -
Human Milk Glycosaminoglycans Inhibit HIV Glycoprotein GP120 Binding to Its Host Cell CD4 Receptor
(1995)The binding of the HIV envelope glycoprotein, gp120, to its host cell receptor, CD4, is inhibited in a solid phase assay by a glycosaminoglycan of human milk; this binding is the essential first step in HIV infectivity. ... -
Human Single Chain Antibodies Against Heparin: Selection, Characterization and Effect on Coagulation
(2002-04-01)Heparin, located in mast cells and basophilic granulocytes, is widely used as an anticoagulant. It belongs to a class of linear polysaccharides called glycosaminoglycans (GAGs). Using phage display technology, we have ... -
Hyaluronan and Heparin Reduced Silver Nanoparticles Having Antimicrobial Properties
(Farrar, Straus and Giroux, 2009-07-13)Aims: Silver nanoparticles exhibit unique antibacterial properties that make these ideal candidates for biological and medical applications. We utilized a clean method involving a single synthetic step to prepare silver ... -
Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building block analysis of low molecular weight heparins prepared through nitrous acid depolymerization
(2017-01-06)Low molecular weight heparins (LMWHs) are important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin. Based on the types of processing reactions and the structures of the products, ... -
Hyphenated techniques for the analysis of heparin and heparan sulfate
(Springer, 2011-01-01)The elucidation of the structure of glycosaminoglycan has proven to be challenging for analytical chemists. Molecules of glycosaminoglycan have a high negative charge and are polydisperse and microheterogeneous, thus ...