Browsing The Linhardt Research Labs by Title
Now showing items 530-549 of 1022
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Importance of Distinct Water Environments in the Hydrolysis of Poly(D,L-lactide-co-glycolide
(1994)A detailed examination of the role of water in the hydrolysis of poly (DL-lactide/glycolide) esters is reported. The hydrolysisrate of polyester pellets was independent of moderate changes in ionic strength, pH, and buffer ... -
Improved Matrix-Assisted Laser Desorption/Ionization Mass Spectrometric Detection of Glycosaminoglycan Disaccharides as Cesium Salts
(Wiley, 2007-04-04)A new method is presented for the identification of oligosaccharides obtained by enzymatic digestion of hyaluronan (HA) with bacterial hyaluronidase (E.C. 4.2.2.1, from Streptomyces hyalurolyticus) using matrix assisted ... -
Improved octyl glucoside synthesis using immobilized β-glucosidase on PA-M with reduced glucose surplus inhibition
(2017-09-03)A β-glucosidase extracted from bitter almond (Prunus dulcis var. amara) was immobilized on polyamine microspheres (PA-M) for catalytic octyl glucoside (OG) synthesis from glucose and octanol through reversed hydrolysis. ... -
Improved viability and thermal stability of the probiotics encapsulated in a novel electrospun fiber mat
(2018-10-17)For the enhancement of the probiotics' survivability, a nanostructured fiber mat was developed by electrospinning. The probiotic Lactobacillus plantarum was encapsulated in the nanofibers with fructooligosaccharides (FOS) ... -
In vitro fermentation behaviors of fucosylated chondroitin sulfate from Pearsonothuria graeffei by human gut microflora
(2017-09-01)A fucosylated chondroitin sulfate (FCS-pg) with highly repeated structure from Pearsonothuria graeffei was subjected to a in vitro fermentation model to investigate its fermentability and effects on human gut microflora. ... -
In Vivo Activity of Microbial Heparinase
(1982)Heparin, prepared from mammalian intestinal mucosa, is the most widely used anticoagulant. It has been estimated, however, that the use of heparin leads to complications such as bleeding 8-33% of the time1. Of all prescription ... -
In Vivo Antithrombotic Synergy of Oral Heparin and Arginine: Endothelial Thromboresistance Without Systemic Anticoagulation
(Thieme Medical Publishers, 2006)On the basis of suggested clinical efficacy in an uncontrolled study in ninety-seven patients with unstable angina, an animal study was conducted to investigate antithrombotic synergy between orally administered heparin ... -
Increase in the Growth Inhibition of Bovine Pulmonary Artery Smooth Muscle Cells by an O-Hexanoyl Low Molecular Weight Heparin Derivative
(Elsevier, 2006-11-06)Proliferation of pulmonary artery smooth muscle cells (PASMCs) appears to play a significant role in chronic pulmonary hypertension. The proliferation of PASMCs is strongly inhibited by some commercial heparin preparations. ... -
Increased 3’-phosphoadenosine-5’-phosphosulfate levels in engineered Escherichia coli cell lysate facilitates in vitro synthesis of chondroitin sulfate A
(Wiley, 2019)Chondroitin sulfates (CSs) are linear glycosaminoglycans that have important applications in the medical and food industries. Engineering bacteria for the microbial production of CS will facilitate a one-step, scalable ... -
Increased CHST15 Expression follows Decline in Arylsulfatase B (ARSB) and Disinhibition of Non-Canonical WNT Signaling: Potential for Epithelial-Mesenchymal Transition
(Impact Journals, 2020)Expression of CHST15 (carbohydrate sulfotransferase 15; chondroitin 4-sulfate-6-sulfotransferase; BRAG), the sulfotransferase enzyme that adds 6-sulfate to chondroitin 4-sulfate (C4S) to make chondroitin 4,6-disulfate ... -
Increased soluble heterologous expression of a rat brain 3-O-sulfotransferase 1 - A key enzyme for heparin biosynthesis
(2018-11-01)Heparan sulfate (HS), is a glycosaminoglycan (GAG) involved in various biological processes, including blood coagulation, wound healing and embryonic development. HS 3-O-sulfotransferases (3-OST), which transfer the sulfo ... -
Induction of nucleolin translocation by acharan sulfate in A549 cells
(2010)Acharan sulfate (AS), isolated from the giant African snail Achatina fulica, is a novel glycosaminoglycan, consisting primarily of the repeating disaccharide structure α-D-N-acetylglucosaminyl (1 → 4) 2-sulfoiduronic acid. ... -
Industrial production of glycosaminoglycans
(2019-01-01)Glycosaminoglycans (GAGs) are naturally occurring polysaccharides, composed of alternating sugar units; these include an amino sugar (e.g., N-acetyl glucosamine, GlcNAc or N-acetyl galactosamine, GalNAc) and either a ... -
Influence of a 3D microarray environment on human cell culture in drug screening systems
(Elsevier, 2012)We have used a modified 3D cellular microarray platform for the high-throughput analysis of growth, cytotoxicity, and protein expression profile of a human hepatocellular carcinoma cell line, HepG2, in alginate. The results ... -
Influence of charge state and sodium cationization on the electron detachment dissociation and infrared multiphoton dissociation of glycosaminoglycan oligosaccharides
(American Chemical Society (ACS), 2008-06-01)Impaired placento-fetal communication is a coherent symptom of exaggerated pre-eclampsia. The impact of the cellular expression of different glycosaminoglycans (GAGs) in this event on the placenta in pre-eclampsia is still ... -
Influence of Formulation Methods on the In Vitro Controlled Release of Protein from Poly(Ester) Microspheres
(1991)Poly (dl-lactide/glycolide, 50:50) microspheres containing bovine serum albumin (BSA) were pre-pared with and without Carbopol® 951 (a potential adjuvant agent) by o/o, o/w and (w/o) /w emul-sion methods. The protein loading ... -
Influence of Heparin Chemical Modifications on Its Antiproliferative Properties
(2005-12-01)This chapter focuses on the mechanisms contributing to heparin inhibition of smooth muscle cell growth. Chronic pulmonary hypertension is characterized by structural changes in the pulmonary vasculature, which along with ... -
Inhibition of Apolipoprotein E- Related Neurotoxicity by Glycosaminoglycans and their Oligosaccharides
(2002-06-25)Apolipoprotein E (apoE) has been genetically linked to late-onset Alzheimer's disease (AD). The role of this lipid-transport protein in AD remains to be established. One hypothesis is that apoE, particularly the apoE4 ... -
Inhibition of glucuronomannan hexamer on the proliferation of lung cancer through binding with immunoglobulin G
(2020-11-15)The anti-lung cancer activity of oligosaccharides derived from glucuronomannan was investigated. The inhibition of A549 cell proliferation by glucuronomannan (Gn) and its oligomers (dimer (G2), tetramer (G4) and hexamer ... -
Inhibition of human vascular NADPH oxidase by apocynin derived oligophenols
(Elsevier, 2009-07-15)Enzymatic oxidation of apocynin, which may mimic in vivo metabolism, affords a large number of oligomers (apocynin oxidation products, AOP) that inhibit vascular NADPH oxidase. In vitro studies of NADPH oxidase activity ...