Browsing The Linhardt Research Labs by Title
Now showing items 589-608 of 1022
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Kartogenin-loaded coaxial PGS/PCL aligned nanofibers for cartilage tissue engineering
(2020-02-01)Electrospinning is a valuable technology for cartilage tissue engineering (CTE) due to its ability to produce fibrous scaffolds mimicking the nanoscale and alignment of collagen fibers present within the superficial zone ... -
Keratan sulfate glycosaminoglycan from chicken egg white
(2016-07-01)Keratan sulfate (KS) was isolated from chicken egg white in amounts corresponding to ∼0.06 wt% (dry weight). This KS had a weight-average molecular weight of ∼36–41 kDa with a polydispersity of ∼1.3. The primary repeating ... -
Kinetic and Structural Studies on Interactions between Heparin/GAGs and Langerin
(2016)Langerin, a C-type lectin, is expressed in Langerhans cells. It was reported that langerin binds sulfated glycans, which is an important initial step for its role in blocking human immunodeficiency virus (HIV) transmission ... -
Kinetic and Structural Studies on Interactions between Heparin/Heparan Sulfate and Proteins of the Hedgehog Signaling Pathway
(American Chemical Society (ACS), 2007-04-03)Heparan sulfate (HS) proteoglycans (PGs) interact with a number of extracellular signaling proteins, thereby playing an essential role in the regulation of many physiological processes. These interactions are important for ... -
Kinetic Studies on the Interaction of Heparin and Complement Proteins using Surface Plasmon Resonance
(Elsevier, 2005-11-15)Heparin is a naturally occurring polysaccharide known to interact with complement proteins and regulate multiple steps in the complement cascade. Quantitative information, in the form of affinity constants for heparin-complement ... -
LC/MS Sequencing of Highly Sulfated Heparin-Derived Oligosaccharides
(Elsevier, 2004)Liquid chromatography/mass spectrometry (LC/MS) is applied to the analysis of complex mixtures of oligosaccharides obtained through the controlled, heparinase-catalyzed depolymerization of heparin. Reversed-phase ion-pairing ... -
Lectin Affinity Electrophoresis
(1995)Lectin affinity electrophoresis is a powerful technique to investigate the interaction between a lectin and its ligand. Affinity electrophoresis results from the reduced mobility of a charged species owing to its interaction ... -
Lectin Affinity Electrophoresis for the Separation of Fluorescently Labeled Sugar Derivatives
(1992-01-01)Lectin affinity electrophoresis was applied to the separation of charged, fluorescent conjugates of disaccharides. Four fluorescent conjugates were prepared by reductive amination of α-d-Man-(1→3)-d-Man, α-d-Gal-(1→4)-d-Gal, ... -
Lessons learned from the contamination of heparin
(Royal Society of Chemistry, 2009-03-09)Heparin is unique as one of the oldest drugs currently still in widespread clinical use as an anticoagulant, a natural product, one of the first biopolymeric drugs, and one of the few carbohydrate drugs. Recently, certain ... -
Lipase Catalyzed Synthesis and Characterization of Poly (Glycerol-Sebacate)
(2022)This study demonstrated that immobilized Candida antarctica lipase B (N435) catalysis in bulk leads to higher molecular weight poly(glycerol sebacate), PGS, than self-catalyzed condensation polymerization. Since the ... -
Lipase-Catalyzed Poly(glycerol-1,8-octanediol-sebacate): Biomaterial Engineering by Combining Compositional and Crosslinking Variables
(2022)This study examined poly(glycerol-1,8-octanediol-sebacate) (PGOS) copolymers with low-level substitution of O (1,8-octanediol) for G (glycerol) units (G/O ratios 0.5:0.5, 0.66:0.33, 0.75:0.25, 0.8:0.2, and 0.91:0.09) ... -
Liquid Chromatography-Mass Spectrometry to Study Chondroitin Lyase Action Pattern
(2009-02-01)Liquid chromatography–mass spectrometry was applied to determine the action pattern of different chondroitin lyases. Two commercial enzymes, chondroitinase ABC (Proteus vulgaris) and chondroitinase ACII (Arthrobacter ... -
Liquid to liquid extraction and liquid chromatography-tandem mass spectrometry determination of hainanmycin in feed
(2017-03-01)Hainanmycin is a new veterinary polyether antibiotic and has few sensitive analytical method in present days. In this study, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) relying on multiple reaction monitoring ... -
Localization and Characterization of Acharan Sulfate in the Body of the Giant African Snail Achatina fulica
(2001-01-01)Acharan sulfate is a glycosaminoglycan (GAG), having the structure -->4)-2-acetamido-2-deoxy-alpha-D-glucopyranose(1-->4)-2-sulfo-alpha-L-idopyranosyluronic acid (1-->, isolated from the body of the giant African snail ... -
Long Duration of Anticoagulant Activity and Protective Effects of Acharan Sulfate in Vivo
(2004-04-26)Introduction: We previously reported that a new glycosaminoglycan, acharan sulfate (AS) from the African giant snail Achatina fulica showed anticoagulant activity in vitro, but was much less active when compared to heparin. ... -
Loss and rescue of osteocalcin and osteopontin modulate osteogenic and angiogenic features of mesenchymal stem/stromal cells
(2020-10-01)Noncollagenous proteins in the bone extracellular matrix, such as osteocalcin (OC) and osteopontin (OPN), inherent to evolution of bone as a skeletal tissue, are known to regulate bone formation and mineralization. However, ... -
Loss of endothelial sulfatase-1 after experimental sepsis attenuates subsequent pulmonary inflammatory responses
(2019-01-01)Sepsis patients are at increased risk for hospital-acquired pulmonary infections, potentially due to postseptic immunosuppression known as the compensatory anti-inflammatory response syndrome (CARS). CARS has been attributed ... -
Loss of Hs3st3a1 or Hs3st3b1 enzymes alters heparan sulfate to reduce epithelial morphogenesis and adult salivary gland function
(Elsevier, 2021-09-01)Heparan sulfate 3-O-sulfotransferases generate highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes on cell surfaces and in the extracellular matrix. Previous ex vivo experiments suggested functional redundancy ... -
Low Molecular Weight Dermatan Sulfate as an Antithrombotic Agent: Structure Activity Relationship Studies
(1994-03-29)A structure-activity relationship of low molecular weight dermatan sulfate was undertaken to understand better this new non-heparin, glycosaminoglycan-based antithrombotic agent. A dermatan sulfate prepared from bovine ... -
Lysostaphin-functionalized cellulose fibers with antistaphylococcal activity for wound healing applications
(2011-12-01)With the emergence of "super bacteria" that are resistant to antibiotics, e.g., methicillin-resistant Staphylococcus aureus, novel antimicrobial therapies are needed to prevent associated hospitalizations and deaths. ...