Browsing The Linhardt Research Labs by Title
Now showing items 629-648 of 1022
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Metabolic bioengineering: Glycans and glycoconjugates
(2018-10-01)The application of metabolic engineering to the production of glycans and glycoconjugates is the subject of this of Emerging Topics in Life Science. The lack of availability of these complex carbohydrate or saccharide ... -
Metabolic engineering and in vitro biosynthesis of natural and non-natural analogues of plant metabolites
(2013)Over the years, natural products from plants and their non-natural derivatives have shown to be active against different types of chronic diseases. However, isolation of such natural products can be limited due to their ... -
Metabolic Engineering of Bacillus megaterium for Heparosan Biosynthesis using Pasteurella multocida Heparosan Synthase, PmHS2
(Springer Nature, 2019-08-12)Background: Heparosan is the unsulfated precursor of heparin and heparan sulfate and its synthesis is typically the first step in the production of bioengineered heparin. In addition to its utility as the starting material ... -
Metabolic engineering of capsular polysaccharides
(2018-10-01)With rising concerns about sustainable practices, environmental complications, and declining resources, metabolic engineers are transforming microorganisms into cellular factories for producing capsular polysaccharides ... -
Metabolic engineering of Chinese hamster ovary cells: Towards a bioengineered heparin
(Elsevier, 2012-03-01)Heparin is the most widely used pharmaceutical to control blood coagulation in modern medicine. A health crisis that took place in 2008 led to a demand for production of heparin from non-animal sources. Chinese hamster ... -
Metabolic engineering of cyanobacteria for photoautotrophic production of heparosan, a pharmaceutical precursor of heparin
(2019-01-01)Heparosan is an unsulfated polysaccharide potentially important for its wide range of cosmetic and pharmaceutical applications, particularly as the precursor for the extensively used anticoagulant, heparin. Generally sourced ... -
Metabolic Engineering of Saccharomyces cerevisiae for High-Level Production of Chlorogenic Acid from Glucose
(2022-02-18)Chlorogenic acid (CGA), a major dietary phenolic compound, has been increasingly used in the food and pharmaceutical industries because of its ready availability and extensive biological and pharmacological activities. ... -
Metachromatic Activity of Heparin and Heparin Fragments
(1984-02-15)Heparin of an average molecular weight of 13,000 was fractionated on the basis of size into five fractions of different weight-average molecular weight ranging from 8500 to 20,000. The heparin was also degraded using ... -
A method for characterizing human intervertebral disc glycosaminoglycan disaccharides using liquid chromatography- mass spectrometry with multiple reaction monitoring
(AO Research Institute Davos, 2018-01-01)Intervertebral disc (IVD) degeneration results in the depletion of proteoglycans and glycosaminoglycans (GAGs), which can lead to structural and mechanical loss of IVD function, ingrowth of nociceptive nerve fibres and ... -
Microanalysis of stomach cancer glycosaminoglycans
(Springer, 2013-10-01)Glycosaminoglycans (GAGS) are anionic, linear, polysaccharides involved in cell signaling. The GAG content, composition and structure of human tissue have been suggested to play a role in cancer and might provide useful ... -
Microarray platform affords improved product analysis in mammalian cell growth studies
(Wiley, 2014-01-01)High throughput (HT) platforms serve as a cost-efficient and rapid screening method for evaluating the effect of cell-culture conditions and screening of chemicals. We report the development of a HT cell-based microarray ... -
Microbially Produced Rhamnolipid as a Source of Rhamnose
(1989-01-01)The sugar L-rhamnose (6-deoxy-~-mannose) is used as a fine chemical in scientific and industrial settings, as a component in chemical reactions, and as a starting material in the synthesis of organic compounds. Present ... -
Microheterogeneity of Plasma Glycoproteins Antithrombin III and Heparin Cofactor II and Their Carbohydrate Analysis
(1988-07-01)Antithrombin III is the most important protease inhibitor in the blood coagulation cascade. Its activity results from the formation of covalent complexes with Factor Xa and thrombin (FIIa) (1). The rate of this inhibition ... -
Microscale Isolation and Analysis of Heparin from Plasma using an Anion Exchange Spin Column
(Elsevier, 2006-06-15)Heparin, a linear highly sulfated polysaccharide, is a member of the glycosaminoglycan (GAG)1 family [1,2]. Since the 1930s, heparin has been widely used as a clinical anticoagulant [3,4]. More recently, low-molecular ... -
Microscale separation of heparosan, heparan sulfate and heparin
(Elsevier, 2013-03-15)The separation and quantification of glycosaminoglycan (GAG) chains, with different levels of sulfation, from cells, media and prepared through chemoenzymatic synthesis or metabolic engineering, poses a major challenge in ... -
Mode of Action of Heparin Lyase On Heparin
(1982-04-03)Heparinase (heparin lyase, EC 4.2.2.7) prepared from Flavobacterium heparinum was used to digest heparin. The products of digestion were examined with a viscosometric assay at various stages of the reaction to measure their ... -
Modification and Potential Uses of Heparin and Other Acidic Polysaccharides
(1990)Heparin lo a polydisperse, highly sulfated, linear polysaccharide of repeating uronic acid and glucosamine residues (Figure 1). Although heperin has been used clinically as an anticoagulant for the past 5O years, its precise ... -
Modification of solid phase red cell adherence assay for the detection of platelet alloantibodies in patients with thrombocytopenia
(Oxford, 2008)Platelet refractoriness is caused by HLA antibodies and platelet-specific antibodies. Current methods used to detect antiplatelet antibodies have limitations. Solid phase red cell adherence (SPRCA) lacks sensitivity and ... -
Modified Assay for the Measurement of Biotin in the Presence of Heparin
(1995-01-01)Tween 20 is frequently used in blotting analysis of proteins and nucleic acids to decrease background staining due to nonspecific binding of antibodies to various membranes. Inclusion of 0.1% (w/v) Tween 20 in the washing ... -
Modulation of heparan sulfate biosynthesis by sodium butyrate in recombinant CHO cells
(2015-03-18)Sodium butyrate, a histone deacetylase inhibitor, has been used to improve transgene expression in Chinese hamster ovary (CHO) cells. The current study explores the impact of butyrate treatment on heparan sulfate (HS) ...