Browsing The Linhardt Research Labs by Title
Now showing items 777-796 of 1022
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Proteoglycans in stem cells
(2012)The remarkable promise of pluripotent and multipotent stem cells (SCs) imparts tremendous optimism for advancement of regenerative medicine, developmental biology, and drug discovery. Perhaps the greatest challenge is to ... -
Proteoglycomics: Recent progress and future challenges
(2010-08-01)Proteoglycomics is a systematic study of structure, expression, and function of proteoglycans, a posttranslationally modified subset of a proteome. Although relying on the established technologies of proteomics and glycomics, ... -
Proteome analysis and selenium-binding peptide identification on proteins sequentially extracted from loach (Misgurnus anguillicaudatus)
(Biochemistry & Physiology, 2017)Loach (Misgurnus anguillicaudatus) is known as a selenium-rich aquatic product. In present study, a sequential extraction method was applied for separation of water, salt and alkali-soluble proteins from loach (Misgurnus ... -
Proteomics of old world camelid (Camelus dromedarius): Better understanding the interplay between homeostasis and desert environment
(Elsevier, 2014)Life is the interplay between structural–functional integrity of biological systems and the influence of the external environment. To understand this interplay, it is useful to examine an animal model that competes with ... -
Purification and Characterization of a Novel Heparinase from Bacteroides stercoris HJ-15
(2000-01-01)A novel type of heparinase (heparin lyase, no EC number) has been purified from Bacteroides stercoris HJ-15, isolated from human intestine, which produces three kinds of heparinases. The enzyme was purified to apparent ... -
Purification and Characterization of Heparan Sulfate Peptidoglycans from Bovine Liver
(Elsevier, 2002)A method for the isolation and purification of peptidoglycan heparan sulfate (HS) from bovine liver is described. Peptidoglycan HS was purified from bovine liver and its structure was characterized. The purity and identity ... -
Purification and Characterization of Heparin Lyases from Flavobacterium heparinum
(Elsevier, 1992)Heparin lyase I has been purified from Flavobacterium heparinum and has been partially characterized (Yang, V. C., Linhardt, R. J., Berstein, H., Cooney, C. L., and Langer, R. (1985) J. Biol. Chem. 260, 1849-1857). There ... -
Purification and Characterization of Heparinase from Flavobacterium heparinum
(Elsevier, 1985)Heparinase (EC 4.2.2.7) isolated from Flavobacterium heparinum was purified to homogeneity by a combination of hydroxylapatite chromatography, repeated gel filtration chromatography, and chromatofocusing. Homogeneity was ... -
Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa
(2018-08-01)Grifola frondosa is a polypore mushroom, which has been used for many centuries by traditional Chinese and Japanese herbalists as a medicinal mushroom. A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: ... -
Purification, Characterization and Specificity of Chondroitin Lyases and Glucuronidase from Flavobacterium heparinum
(1995)The chondroitin lyases from Flavobacterium heparinum (Cytophaga heparinia) have been widely used in depolymerization of glycosaminoglycan and proteoglycan chondroitin sulphates. Oligosaccharide products derived from ... -
Qualitative and quantitative analysis of branches in dextran using high-performance anion exchange chromatography coupled to quadrupole time-of-flight mass spectrometry
(2015-12-04)Dextran, a family of natural polysaccharides, consists of an α (1→6) linked-glucose main (backbone) chain having a number of branches. The determination of the types and the quantities of branches in dextran is important ... -
Qualitative and quantitative analysis of heparin and low molecular weight heparins using size exclusion chromatography with multiple angle laser scattering/refractive index and inductively coupled plasma/mass spectrometry detectors
(2017-11-03)Heparin, a highly sulfated glycosaminoglycan, has been used as a clinical anticoagulant over 80 years. Low molecular weight heparins (LMWHs), heparins partially depolymerized using different processes, are widely used as ... -
Qualitative and Quantitative Determination of Ten Major Saponins in Platycodi Radix by High Performance Liquid Chromatography with Evaporative Light Scattering Detection and Mass Spectrometry
(Elsevier, 2006-11-24)Saponins in Platycodi Radix (platycosides) exhibit potent biological activities in mammalian systems, including several beneficial effects such as anti-inflammatory, immunomodulatory and anti-obesity activities. In this ... -
Quantitation of heparosan with heparin lyase III and spectrophotometry
(2014-02-15)Heparosan is Escherichia coli K5 capsule polysaccharide, which is the key precursor for preparing bioengineered heparin. A rapid and effective quantitative method for detecting heparosan is important in the large-scale ... -
Quantitative Analysis of Chondroitin Sulfate in Raw Materials, Ophthalmic Solutions, Soft Capsules and Liquid Preparations
(2005-04-25)We performed the quantitative analysis of chondroitin sulfate (CS) obtained from raw materials and various pharmaceutical preparations. To quantify CS content in raw materials and in an ophthalmic solution, each test sample ... -
Quantitative analysis of heparan sulfate using isotopically labeled calibrants
(2020)Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions. Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount ... -
Quantitative analysis of the major linkage region tetrasaccharides in heparin
(2017-02-10)Heparin is a polysaccharide based anticoagulant drug composed of a complex mixture of glycosaminoglycan chains and peptidoglycosaminoglycan chains. In an effort to better characterize this important polysaccharide based ... -
Quantitative capillary electrophoresis determination of oversulfated chondroitin sulfate as a contaminant in heparin preparations
(Elsevier, 2009-05-01)A simple, accurate, and robust quantitative capillary electrophoresis (CE) method for the determination of oversulfated chondroitin sulfate (OSCS) as a contaminant in heparin (Hep) preparations is described. After degradation ... -
Quantitative Continuous Assay for Hyaluronan Synthase
(Elsevier, 2007-02-15)A rapid, continuous, and convenient three-enzyme coupled UV absorption assay was developed to quantitate the glucuronic acid and N-acetylglucosamine transferase activities of hyaluronan synthase from Pasteurella multocida ... -
Quantitification of Heparan Sulfate and Heparin Disaccharides Using Ion-pairing, Reverse-Phase, Micro-flow, High Performance Liquid Chromatography Coupled with Electrospray Ionization Trap Mass Spectrometry
(American Chemical Society (ACS), 2009)The glycosaminoglycan (GAG) family of biomacromolecules is composed acidic and linear chains of repeating disaccharide units. Quantitative disaccharide composition analysis is essential for the study and characterization ...