Browsing The Linhardt Research Labs by Title
Now showing items 817-836 of 1022
-
Regioselective Synthesis of Sucrose Monoesters as Surfactants
(1997-01-01)A highly regioselective conversion of sucrose into 6-O-acyl derivatives is reported. First sucrose was transformed into the dibutyltin acetal, thus enhancing the nucleophilicity at the C-6 oxygen and restricting the ... -
Regulating malonyl-CoA metabolism via synthetic antisense RNAs for enhanced biosynthesis of natural products
(2015-05-01)Malonyl-CoA is the building block for fatty acid biosynthesis and also a precursor to various pharmaceutically and industrially valuable molecules, such as polyketides and biopolymers. However, intracellular malonyl-CoA ... -
Regulation Activity of Heparin in Complement System
(2005)Heparin (HP) can bind to a variety of proteins, including growth factors, pro-inflammatory chemokines and cytokines, extracellular matrix proteins, and complement proteins. HP has a variety of biological activities, many ... -
Regulation of PTP1B activation through disruption of redox-complex formation
(Nature, 2020-02-01)We have identified a molecular interaction between the reversibly oxidized form of protein tyrosine phosphatase 1B (PTP1B) and 14-3-3ζ that regulates PTP1B activity. Destabilizing the transient interaction between 14-3-3ζ ... -
Release and Preparation of Intact and Unreduced N-linkedOligosaccharides from Sf-9 Insect Cells
(1999)Glycosylation, the addition of carbohydrates to a peptide backbone, is the most extensive cotranslational and posttranslational modification made to proteins by eukaryotic cells. The glycosylation profile of a recombinant ... -
Remodeling of Glycosaminoglycans During Differentiation of Adult Human Bone Mesenchymal Stromal Cells toward Hepatocytes
(2019-02-15)There is a critical need to generate functional hepatocytes to aid in liver repair and regeneration upon availability of a renewable, and potentially personalized, source of human hepatocytes (hHEP). Currently, the vast ... -
Repurposing paper by-product lignosulfonate as sulfur donor/acceptor for high performance lithium-sulfur batteries
(2018-01-01)The recovery and repurposing of biomass are critically important and make a significant contribution to environmental preservation. Lignosulfonate, a by-product of the paper manufacturing industry, is an abundant low cost ... -
Resistance of Heparinase-Derived Heparin Fragments to Biotransformation
(Elsevier, 1989)The biotransformation of heparinase-derived heparin fragments was examined via a combined approach using 35S-labeled heparin fragments as well as unlabeled chemically defined heparin fragments. Rats dosed with either ... -
Resolution of Structurally Similar Glycoproteins by Affinity-BasedReversed Micellar Extraction and Separation
(1998)Affinity-based reversed micellar extraction and separation (ARMES) has proven effective in separating glycoproteins from nonglycosylated proteins from natural sources. The ability of ARMES to resolve closely related ... -
Response surface optimization of the heparosan N-deacetylation in producing bioengineered heparin
(Elsevier, 2011-12-10)The chemical step in the chemoenzymatic synthesis of bioengineered heparin has been examined and optimized statistically using a response surface methodology. A four factor, two level full factorial design experiment and ... -
The responses of hyperglycemic dividing mesangial cells to heparin is mediated by the non-reducing terminal trisaccharide
(American Society for Biochemistry and Molecular Biology (ASBMB) and Elsevier, 2015-11-27)Our previous studies showed: (i) that growth-arrested G0/G1 rat mesangial cells stimulated to divide in hyperglycemic medium initiate intracellular hyaluronan synthesis that induces autophagy and the cyclin D3-induced ... -
Rethinking the impact of RG-I mainly from fruits and vegetables on dietary health
(2020-09-24)Rhamnogalacturonan I (RG-I) pectin is composed of backbone of repeating disaccharide units →2)-α-L-Rhap-(1→4)-α-D-GalpA-(1→ and neutral sugar side-chains mainly consisting of arabinose and galactose having variable types ... -
Review on the impact of polyols on the properties of biobased polyesters
(Multidisciplinary Digital Publishing Institute (MDPI), 2020)Bio-based polyol polyesters are biodegradable elastomers having potential utility in soft tissue engineering. This class of polymers can serve a wide range of biomedical applications. Materials based on these polymers are ... -
A revised structure for the glycolipid terminus of E. coli K5 heparosan capsular polysaccharide
(Multidisciplinary Digital Publishing Institute (MDPI), 2020)The structure of heparosan capsular polysaccharide (CPS) has been determined using enzymatic digestion with nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. Previous errors in the assignment of the ... -
RNA aptamers with specificity for heparosan and chondroitin glycosaminoglycans
(American Chemical Society (ACS), 2018-10-10)In this study, two respective groups of RNA aptamers have been selected against two main classes of glycosaminoglycans (GAGs), heparosan, and chondroitin, as they have proven difficult to specifically detect in biological ... -
Role of Arginine 292 in the Catalytic Activity of Chondroitin AC Lyase from Flavobacterium heparinum
(2002-06-03)Chondroitin AC lyase (chondroitinase EC 4.2.2.5), an eliminase from Flavobacterium heparinum, cleaves chondroitin sulfate glycosaminoglycans (GAGs) at 1,4 glycosidic linkages between N-acetylgalactosamine and glucuronic ... -
Role of Glycosaminoglycans in Cellular Communication
(2004-07-01)Glycosaminoglycans are of critical importance in intercellular communication in organisms. This ubiquitous class of linear polyanions interacts with a wide variety of proteins, including growth factors and chemokines, which ... -
Room Temperature Ionic Liquids as Emerging Solvents for the Pretreatment of Lignocellulosic Biomass
(2011-06-01)Room temperature ionic liquids (RTILs) are emerging as attractive and green solvents for lignocellulosic biomass pretreatment. The unique solvating properties of RTILs foster the disruption of the 3D network structure of ... -
Search for the Heparin Antithrombin III Binding Site Precursor
(Elsevier, 1992)The last step of heparin biosynthesis is thought to involve the action of 3-O-sulfotransferase resulting in the formation of an antithrombin III (ATIII) binding site required for heparin's anticoagulant activity. The ... -
Search for the Heparin Antithrombin III Binding Site Precursor
(Elsevier, 1992)The last step of heparin biosynthesis is thought to involve the action of 3-O-sulfotransferase resulting in the formation of an antithrombin III (ATIII) binding site required for heparin's anticoagulant activity. The ...