Browsing The Linhardt Research Labs by Title
Now showing items 985-1004 of 1022
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The Role of the Methoxyphenol Apocynin, a Vascular NADPH Oxidase Inhibitor, as a Chemopreventative Agent in the Potential Treatment of Cardiovascular Diseases
(2008-12-01)Oxidative stress has been linked to the origin and progression of cardiovascular diseases. Nicotinamide adenine dinucleotide phosphate, reduced form (NADPH) oxidase is a multi-component, NADPH-dependent enzyme that generates ... -
The Stereospecific Synthesis of KDN a-C-glycosides by Samarium-Mediated Reductive Desulfonylation ofGlycosyl Phenylsulfone
(1998)KDN, 3-deoxy-D-glycero-D-galacto-2-nonulopyranosylonic acid, is a novel type of sialic acid in which the acetamido group at C-S of N-acetylneuraminic acid is replaced by a hydroxyl group. This ulosonic acid was first ... -
The structure-activity relationship of the interactions of SARS-CoV-2 spike glycoproteins with glucuronomannan and sulfated galactofucan from Saccharina japonica
(2020-11-15)The SARS-CoV-2 spike glycoproteins (SGPs) and human angiotensin converting enzyme 2 (ACE2) are the two key targets for the prevention and treatment of COVID-19. Host cell surface heparan sulfate (HS) is believed to interact ... -
The US regulatory and pharmacopeia response to the global heparin contamination crisis
(Nature, 2016-06-09)The contamination of the widely used lifesaving anticoagulant drug heparin in 2007 has drawn renewed attention to the challenges that are associated with the characterization, quality control and standardization of complex ... -
Thermodynamic Analysis of Heparin Interaction with a Basic CyclicProtein Using Isothermal Titration Calorimetry
(1998)Brain natriuretic peptide (BNP) was examined as part of a continuing study of the interaction of proteins and peptides with the glycosaminoglycan heparin. BNP was tentatively identified as a heparin-binding protein on the ... -
Thin Layer Chromatography for the Analysis of Glycosaminoglycan Oligosaccharides
(Elsevier, 2007-12-01)Thin layer chromatography was used to analyze glycosaminoglycan oligosaccharides obtained through the use of polysaccharide lyases. This method allows for the rapid, semi-quantitative analysis of a wide variety of ... -
Thin Layer Chromatography for the Separation and Analysis of Acidic Carbohydrates
(2009-10-13)Thin layer chromatography (TLC) is routinely used by synthetic organic chemists and natural products chemists working on a variety of types of molecules. Carbohydrate chemists and biochemists in the past largely relied on ... -
Three Dimensional Cellular Microarray Platform for Human Neural Stem Cell Differentiation and Toxicology
(Elsevier, 2014-01-01)We developed a three-dimensional (3D) cellular microarray platform for the high-throughput (HT) analysis of human neural stem cell (hNSC) growth and differentiation. The growth of an immortalized hNSC line, ReNcell VM, was ... -
Tip-enhanced Raman imaging of single-stranded DNA with single base resolution
(2019-01-16)Tip-enhanced Raman scattering (TERS) is a promising optical and analytical technique for chemical imaging and sensing at single molecule resolution. In particular, TERS signals generated by a gap-mode configuration where ... -
Tissue Distribution [14 C] Sucrose Octasulfate Following Oral Administration to Rats
(2002)Purpose: Aluminum sucrose octasulfate (SOS) is used clinically to prevent ulcers. Under physiologic conditions, the sodium salt of this drug can be formed. Our objective was to determine whether sodium SOS was absorbed ... -
Tissue Distribution and Antithrombotic Activity of Unlabeled or 14C-Labelled Porcine Intestinal Mucosal Heparin Following Administration to Rats by the Oral Route
(2000-01-01)Distribution and antithrombotic activity of orally administered unfractionated porcine heparin were studied. [14C]Heparin was prepared by de-N-acetylation of porcine mucosal heparin followed by re-N-acetylation, using ... -
Toll-like Receptor 4 Mediates Induction of Inflammatory Pathway by Food Additive Carrageenan
(2008)The sulfated polysaccharide carrageenan (CGN) induces activation of NFκB and interleukin 8 (IL-8) in human colonic epithelial cells through a pathway of innate immunity mediated by Bcl10 (B-cell CLL/lymphoma 10). In this ... -
Top-down approach for the direct characterization of low molecular weight heparins using LC-FT-MS
(American Chemical Society (ACS), 2012-10-16)Low molecular heparins (LMWHs) are structurally complex, heterogeneous, polydisperse, and highly negatively charged mixtures of polysaccharides. The direct characterization of LMWH is a major challenge for currently available ... -
Total Synthesis of Quercetin 3-Sophorotrioside
(2004-03-19)5,7-dihydroxy-3-[beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranosyl]-2-(3,4-dihydroxyphenyl)-4H-1-benzopyran-4-one (quercetin 3-sophorotrioside), a flavonol triglycoside, isolated from Pisum ... -
Toward the chemoenzymatic synthesis of heparan sulfate oligosaccharides: Oxidative cleavage of p-nitrophenyl group with ceric ammonium salts
(Elsevier, 2013-08-14)We have developed an efficient chemoenzymatic synthesis of heparan sulfate oligosaccharides employing the para-nitrophenyl (p-NP) β-glucuronide as an acceptor compatible with enzymatic elongation and one that significantly ... -
Trifluoroethylsulfonate Protected Monosaccharides in Glycosylation Reactions
(2004-08-16)A variety of sulfo-protected monosaccharide donors and acceptors were investigated in glycosylation reactions. Trifluoroethylsulfonate (SO3TFE) group was compatible with a wide range of activation conditions commonly used ... -
Trimer hydroxylated quinone (IIIHyQ) derived from apocynin targets cysteine residues of p47phox preventing the activation of human vascular NADPH oxidase
(Elsevier, 2012)Enzymatically derived oligophenols from apocynin can be effective inhibitors of human vascular NADPH oxidase (Nox). An isolated trimer hydroxylated quinone (IIIHyQ) has been shown to inhibit endothelial NADPH oxidase with ... -
Tunable thermo-responsive poly(N-vinylcaprolactam) cellulose nanofibers: Synthesis, characterization, and fabrication of potential affinity membranes
(2013)Temperature-responsive PVCL homopolymers and functional PVCL polymers containing carboxylic acids are prepared in organic and aqueous solutions. PVCL bulk polymers are characterized using 1H NMR, photometry, ATR-FTIR, and ... -
Turkey Intestines as a Commercial Source of Heparin? Comparative Structural Studeis of Intestinal Avian and Mammalian Glycosaminoglycans
(2003)Heparin is a glycosaminoglycan (GAG) that is extracted primarily from porcine intestinal tissues and is widely used as a clinical anticoagulant. It is biosynthesized as a proteoglycan and stored exclusively in mast cells ... -
Two-Dimension Affinity Resolution Electrophoresis Demonstrates that Three Distinct Heparin Populations Interact with Antithrombin III
(1995)Tween 20 is frequently used in blotting analysis of proteins and nucleic acids to decrease background staining due to nonspecific binding of antibodies to various membranes. Inclusion of 0.1% (w/v) Tween 20 in the washing ...