Browsing The Linhardt Research Labs by Title
Now showing items 999-1018 of 1022
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Toward the chemoenzymatic synthesis of heparan sulfate oligosaccharides: Oxidative cleavage of p-nitrophenyl group with ceric ammonium salts
(Elsevier, 2013-08-14)We have developed an efficient chemoenzymatic synthesis of heparan sulfate oligosaccharides employing the para-nitrophenyl (p-NP) β-glucuronide as an acceptor compatible with enzymatic elongation and one that significantly ... -
Trifluoroethylsulfonate Protected Monosaccharides in Glycosylation Reactions
(2004-08-16)A variety of sulfo-protected monosaccharide donors and acceptors were investigated in glycosylation reactions. Trifluoroethylsulfonate (SO3TFE) group was compatible with a wide range of activation conditions commonly used ... -
Trimer hydroxylated quinone (IIIHyQ) derived from apocynin targets cysteine residues of p47phox preventing the activation of human vascular NADPH oxidase
(Elsevier, 2012)Enzymatically derived oligophenols from apocynin can be effective inhibitors of human vascular NADPH oxidase (Nox). An isolated trimer hydroxylated quinone (IIIHyQ) has been shown to inhibit endothelial NADPH oxidase with ... -
Tunable thermo-responsive poly(N-vinylcaprolactam) cellulose nanofibers: Synthesis, characterization, and fabrication of potential affinity membranes
(2013)Temperature-responsive PVCL homopolymers and functional PVCL polymers containing carboxylic acids are prepared in organic and aqueous solutions. PVCL bulk polymers are characterized using 1H NMR, photometry, ATR-FTIR, and ... -
Turkey Intestines as a Commercial Source of Heparin? Comparative Structural Studeis of Intestinal Avian and Mammalian Glycosaminoglycans
(2003)Heparin is a glycosaminoglycan (GAG) that is extracted primarily from porcine intestinal tissues and is widely used as a clinical anticoagulant. It is biosynthesized as a proteoglycan and stored exclusively in mast cells ... -
Two-Dimension Affinity Resolution Electrophoresis Demonstrates that Three Distinct Heparin Populations Interact with Antithrombin III
(1995)Tween 20 is frequently used in blotting analysis of proteins and nucleic acids to decrease background staining due to nonspecific binding of antibodies to various membranes. Inclusion of 0.1% (w/v) Tween 20 in the washing ... -
Ultra-High Resolution Separation Comes of Age
(2002-11-15)Born in the late 1960s (1), popularized in its infancy for its high resolution (2), and reaching puberty in the mid 1980s with the introduction of commercial instrumentation (3), capillary electrophoresis (CE) has finally ... -
Ultraperformance ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for heparin disaccharide analysis
(Elsevier, 2011)A high resolution method for the separation and analysis of disaccharides prepared from heparin and heparan sulfate (HS) using heparin lyases is described. Ultraperformance liquid chromatography in a reverse-phase, ion-pairing ... -
Ultraperformance liquid chromatography with electrospray ion trap mass spectrometry for chondroitin disaccharide analysis
(Elsevier, 2010-02-01)Chondroitin sulfate (CS) has an important role in cell division, in the central nervous system, and in joint-related pathologies such as osteoarthritis. Due to the complex chemical structure and biological importance of ... -
Ultrasensitive detection and quantification of acidic disaccharides using capillary electrophoresis and quantum dot-based fluorescence resonance energy transfer
(American Chemical Society (ACS), 2013-10-01)Rapid and highly sensitive detection of the carbohydrate components of glycoconjugates is critical for advancing glycobiology. Fluorescence (or Förster) resonance energy transfer (FRET) is commonly used in detection of ... -
Ultrasound-assisted fast preparation of low molecular weight fucosylated chondroitin sulfate with antitumor activity
(2019-04-01)Fucosylated chondroitin sulfate from sea cucumber Isostichopus badionotus (fCS-Ib) was depolymerized with an ultrasound-accelerated, metal-free Fenton chemistry, relying on H2O2/ascorbic acid redox system. Fragments of ... -
Uniform nanoparticle coating of cellulose fibers during wet electrospinning
(2014-09-28)This work outlines a newly developed method that allows electrospun cellulose fibers to be coated with nanoparticles during dry-jet wet electrospinning. Cellulose fibers were wet electrospun from a room temperature ionic ... -
Unique cell surface mannan of yeast pathogen Candida auris with selective binding to IgG
(2020-05-08)The emerging, multidrug-resistant yeast pathogen Candida auris is responsible for healthcare-associated outbreaks across the globe with high mortality. The rapid spread of C. auris is linked to its successful colonization ... -
Unnatural polyketide analogs selectively target the HER2 signaling pathway in human breast cancer cells
(European Chemical Societies, 2010)Receptor tyrosine kinases are critical targets for the regulation of cell survival. Cancer patients with abnormal receptor tyrosine kinases (RTK) tend to have more aggressive disease with poor clinical outcomes. As a result, ... -
UP-HILIC-MS/MS to determine the action pattern of Penicillium sp. dextranase
(2015-07-22)Investigation of the action pattern of enzymes acting on carbohydrates is challenging, as both the substrate and the digestion products are complex mixtures. Dextran and its enzyme-derived oligosaccharides are widely used ... -
Urinary glycosaminoglycans predict outcomes in septic shock and ARDS
(2016)Rationale: Degradation of the endothelial glycocalyx, a glycosaminoglycan (GAG)-rich layer lining the vascular lumen, is associated with the onset of kidney injury in animal models of critical illness. It is unclear if ... -
Urinary metabolomics analysis reveals the anti-diabetic effect of stachyose in high-fat diet/ streptozotocin - induced type 2 diabetic rats
(2020-02-01)As a new platform of systems biology, metabolomics provides a powerful approach to discover therapeutic biomarkers and mechanism of metabolic disease. Type 2 diabetes mellitus (T2DM) is a global metabolic disease, thus, a ... -
Using a 3-O-Sulfated Heparan Sulfate Octasaccharide to Inhibit the Entry of Herpes Simplex Virus 1
(American Chemical Society (ACS), 2008)Heparan sulfate (HS) is a highly sulfated polysaccharide and is present in large quantities on the cell surface and in the extracellular matrix. Herpes simplex virus type 1(HSV-1) utilizes a specialized cell surface HS, ... -
Variation of Acharan Sulfate and Monosaccharide Composition and Analysis of Neutral N-Glycans in African Giant Snail (Acatina fulica)
(Springer, 2008)Acharan sulfate content from African giant snail (Achatina fulica) was compared in eggs and snails of different ages. Acharan sulfate was not found in egg. Acharan sulfate disaccharide -->4)-alpha-D-GlcNpAc (1-->4)-alpha ... -
Viral and Cellular Determinants of Hepatitis C Virus Envelope-Heparan Sulfate Interaction
(2006-11-01)Cellular binding and entry of hepatitis C virus (HCV) are the first steps of viral infection and represent a major target for antiviral antibodies and novel therapeutic strategies. We have recently demonstrated that heparan ...