Browsing The Linhardt Research Labs by Title
Now showing items 1-20 of 1022
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13C-NMR Relaxation Study of Heparin Disaccharide with Tripeptides GRG and GKG
(1996-04-15)Heparin is a polydisperse sulphated copolymer consisting mostly of 1-->4 linked glucosamine and uronic acid residues, i.e. 2-deoxy-2-sulphamido-D-glucopyranose 6-sulphate and L-idopyranosyluronic acid 2-sulphate. 13C NMR ... -
1D and 2D-HSQC NMR: two methods to distinguish and characterize heparin from different animal and tissue sources
(Frontiers Media SA, 2019-06-27)The US Food and Drug Administration has encouraged the reintroduction of bovine heparin drug product to the US market to mitigate the risks of heparin shortages and potential adulteration or contamination of the primary ... -
2-O-Sulfated domains in syndecan-1 heparan sulfate inhibit neutrophil cathelicidin and promote S. aureus corneal infection
(The American Society for Biochemistry and Molecular Biology (ASBMB) and Elsevier, 2015)Ablation of syndecan-1 in mice is a gain of function mutation that enables mice to significantly resist infection by several bacterial pathogens. Syndecan-1 shedding is induced by bacterial virulence factors, and inhibition ... -
The 2.8 Å Electron Microscopy Structure of Adeno-Associated Virus-DJ Bound by a Heparanoid Pentasaccharide
(Elsevier, 2017)Atomic structures of adeno-associated virus (AAV)-DJ, alone and in complex with fondaparinux, have been determined by cryoelectron microscopy at 3 Å resolution. The gene therapy vector, AAV-DJ, is a hybrid of natural ... -
3-O-sulfation of Heparan Sulfate Enhances Tau Interaction and Cellular Uptake
(Wiley, 2020-01-27)Prion-like transcellular spreading of tau in Alzheimer's Disease (AD) is mediated by tau binding to cell surface heparan sulfate (HS). However, the structural determinants for tau-HS interaction are not well understood. ... -
4-O-Sulfation in sea cucumber fucodians contribute to reversing dyslipidiaemia caused by HFD
(Elsevier, 2017-06-01)Fucodians extracted from sea cucumbers (SC-FUCs) possess linear chains with uniform repeating units. Sulfation patterns endow SC-FUCs unique structures and bioactivity. The present study investigated the anti-hyperlipidemic ... -
A Colorimetric Assay for Chemical Heparin in Plasma
(1982-07-15)A simple, rapid colorimetric assay for plasma heparin is presented. The assay employs the metachromasia of azure A when heparin is added. It is useful for 0 to 10 units/ml and does not depend on heparin's anticoagulant ... -
A comparative secretome analysis of industrial Aspergillus oryzae and its spontaneous mutant ZJGS-LZ-21
(2017-05-02)Aspergillus oryzae koji plays a crucial role in fermented food products due to the hydrolytic activities of secreted enzymes. In the present study, we performed a comparative secretome analysis of the industrial strain of ... -
A Dose Escalation Study or Org 10172 (Low Molecular Weight Heparinoid) in Stroke
(1989-01-01)An intravenous infusion of a low molecular weight heparinoid, with a reduced risk of hemorrhage, may be an alternative to heparin in the management of acute ischemic stroke. To evaluate this hypothesis, we studied the ... -
A Flexible Carbon/Sulfur-Cellulose Core-Shell Structure for Advanced Lithium–Sulfur Batteries
(2018-11-01)Lithium sulfur (Li-S) batteries are viewed as a promising candidate for next-generation energy storage systems due to their high energy density, low cost and ease of manufacturing. However, rapid capacity decay caused by ... -
A fucoidan from sea cucumber Pearsonothuria graeffei with well-repeated structure alleviated gut microbiota dysbiosis and metabolic syndromes in HFD-fed mice
(2018)A high-fat diet (HFD) has been a major contributor to increasing morbidity caused by metabolic syndromes. Functional foods from natural sources are potential choices for addressing metabolic diseases because they provide ... -
A General Method for the Stereospecific Synthesis of C-glycosidesof Ulosonic Acids by Samarium-Mediated Reductive Dechlorination
(1998)C-glycosylation reactions were performed with the 2-chloroderivatives of NANA, KDN and KDO. C-glycosides were obtainedwhen carbonyl compounds (ketone or sugar aldehyde) were coupled tothe 2-chloro-derivatives ... -
A Highly Stable Covalent Conjugated Heparin Biochip for Heparin-Protein Interactions Studies
(2002-05-15)Heparin is a proteoglycan composed of highly sulfated linear polysaccharides of alternating uronic acid and glucosamine that interacts with a wide variety of proteins and peptides (1). Heparin and the structurally related ... -
A Kinetic Model for FGF, FGFR and Proteoglycan Signal Transduction Complex Assembly
(2004)The current working model for fibroblast growth factor receptor (FGFR) dimerization and activation requires the assembly of a ternary complex of fibroblast growth factor (FGF), FGFR, and heparin or heparan sulfate proteoglycan ... -
A Molecular Hero Suit for In Vitro and In Vivo DNA Nanostructures
(2019-06-26)Precise control of DNA base pairing has rapidly developed into a field full of diverse nanoscale structures and devices that are capable of automation, performing molecular analyses, mimicking enzymatic cascades, biosensing, ... -
A mutant cell library for systematic analysis of heparan sulfate
(Nature, 2018-11-01)Heparan sulfate (HS) is a complex linear polysaccharide that modulates a wide range of biological functions. Elucidating the structure–function relationship of HS has been challenging. Here we report the generation of an ... -
A New Method for Sequencing Linear Oligosaccharides on Gels Using Charged, Fluorescent Conjugates
(1991-08-18)A new method is described for sequencing linear oligosaccharides on gels using charged, fluorescent conjugates. The reducing ends of various mono-, di-, tri-, and tetra-saccharides were conjugated with monopotassium ... -
A Novel Fabrication of Poly (e-caprolactone)Microspheres from Blends of Poly (e-caprolactone)and Poly (ethylene glycol) s
(1999)A novel method for the preparation of solvent-free poly(ε-caprolactone) (PCL) microspheres from PCL/poly(ethylene glycol) (PEG) blends was developed and demonstrated. The particle size of the PCL spheres are in the range ... -
A novel laminin-binding protein mediates microbial-endothelial cell interactions and facilitates dissemination of Lyme disease pathogens
(2020-04-07)Borrelia burgdorferi conserved gene products BB0406 and BB0405, members of a common B. burgdorferi paralogous gene family, share 59% similarity. Although both gene products can function as potential porins, only BB0405 is ... -
A novel route for double-layered encapsulation of probiotics with improved viability under adverse conditions
(2020-04-25)To improve the survivability of probiotics under the harsh conditions, a novel double-layered vehicle, which was developed by a one-step coaxial electrospinning procedure, was here used to encapsulate the probiotics. The ...