Browsing The Linhardt Research Labs by Title
Now showing items 448-467 of 1022
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HClO4-SiO2 Catalyzed Glycosylation using Sugar Trichloroacetimidates as Glycosyl Donors
(2006-01-16)Silica-supported perchloric acid (HClO4–SiO2) has been used as an efficient promoter, as a replacement of TMSOTf, in various glycosylation reactions using sugar trichloroacetimidates as glycosyl donors. Operational simplicity, ... -
Heavy chain transfer by tumor-necrosis-factor-stimulated-gene-6 to the bikunin proteoglycan
(The American Society for Biochemistry and Molecular Biology (ASBMB) and Elsevier, 2015-02-20)We present data that hyaluronan (HA) polysaccharides, about 14-86 monosaccharides in length, are capable of accepting only a single heavy chain (HC) from inter-α-inhibitor via transfer by tumor necrosis factor-stimulated ... -
Heavy heparin: A stable isotope-enriched, chemoenzymatically-synthesized, poly-component drug
(Wiley, 2019-04-23)Heparin is a highly sulfated, complex polysaccharide and widely used anticoagulant pharmaceutical. In this work, we chemoenzymatically synthesized perdeuteroheparin from biosynthetically enriched heparosan precursor obtained ... -
Heparan Sulfate Domains Required for Fibroblast Growth Factor 1 and 2 Signaling through Fibroblast Growth Factor Receptor 1c
(The American Society for Biochemistry and Molecular Biology (ASBMB) and Elsevier, 2017-02-10)A small library of well defined heparan sulfate (HS) polysaccharides was chemoenzymatically synthesized and used for a detailed structure-activity study of fibroblast growth factor (FGF) 1 and FGF2 signaling through FGF ... -
Heparan sulfate facilitates spike protein-mediated SARS-Cov-2 host cell invasion and contributes to increased infection of SARS-Cov-2 G614 mutant and in lung cancer
(Multidisciplinary Digital Publishing Institute (MDPI), 2021-06-11)The severe acute respiratory syndrome (SARS)-like coronavirus disease (COVID-19) is caused by SARS-CoV-2 and has been a serious threat to global public health with limited treatment. Cellular heparan sulfate (HS) has been ... -
Heparan sulfates from bat and human lung and their binding to the spike protein of SARS-CoV-2 virus
(2021-05-15)Severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2) has resulted in a pandemic and continues to spread at an unprecedented rate around the world. Although a vaccine has recently been approved, there are ... -
Heparin Accelerates Gelsolin Amyloidogenesis
(American Chemical Society (ACS), 2006)The chemical environment of the extracellular matrix may influence the tissue-selective deposition observed there in gelsolin amyloid disease. Previously, we have identified the proteases that generate the amyloidogenic ... -
Heparin and Derivatized Heparin Inhibit Cobra Venom Factor Activation of Complement in Serum
(1994-01-01)Heparin has been shown to inhibit activity of the alternative, classical and terminal pathways of complement by regulating C1, C1 inhibitor, C4 binding protein, C3b, factor H and S-protein. In vivo, heparin inhibits cobra ... -
Heparin and homogeneous model heparin oligosaccharides form distinct complexes with protamine: Light scattering and zeta potential analysis
(2017-06-05)Large multimolecular complexes of heparin with positively charged proteins such as platelet factor 4 (PF4) or protamine can initiate immune responses associated with heparin use in patients, including the most significant ... -
Heparin and related polysaccharides: Synthesis using recombinant enzymes and metabolic engineering
(Springer, 2015-09-22)Glycosaminoglycans are linear anionic polysaccharides that exhibit a number of important biological and pharmacological activities. The two most prominent members of this class of polysaccharides are heparin/heparan sulfate ... -
Heparin as a Potential Therapeutic Agent to Reverse Vascular Remodeling
(2003)Vascular changes after chronic hypoxia are characterized by hyperplasia, hypertrophy, and migration of smooth muscle cells (SMCs) in the media of muscular and partially muscular pulmonary arteries (1,2). A number of factors ... -
Heparin Binding and Augmentation of C1 Inhibitor Activity
(1999-01-15)Heparin and other glycosaminoglycans have profound activity in vitro on the regulation of complement activity. The studies reported here examined the mechanism whereby heparin enhances C1 esterase inhibitor (C1INH) activity ... -
Heparin Binding Domains in Vascular Biology
(American Heart Association AHA/ASA Journals, 2004-09-01)Heparin is a major anticoagulant with activity mediated primarily through its interaction with antithrombin (AT). Heparan sulfate (HS), structurally related to heparin, binds a wide range of proteins of different functionality, ... -
Heparin Contamination and Issues Related to Raw Materials and Controls
(2019-01-01)Heparin is a century-old polypharmacological drug critical for the practice of modern medicine. In recent years, there have been a number of issues arising in the preparation of heparin from food animal tissues. The most ... -
Heparin Dodecasaccharide Binding to Platelet Factor-4 and Growth-RelatedProtein-a: Transition through a Partially Folded State and Implications for Heparin Induced Thrombocytopenia
(Elsevier, 1999)alpha-Chemokines are known heparin-binding proteins. Here, a heparin dodecasaccharide (H12) was purified and used in NMR studies to investigate binding to growth-related protein-alpha (Gro-alpha) and to platelet factor-4-M2 ... -
Heparin mapping using heparin lyases and the generation of a novel low molecular weight heparin
(American Chemical Society (ACS), 2011-01-27)Seven pharmaceutical heparins were investigated by oligosaccharide mapping by digestion with heparin lyase 1, 2, or 3, followed by high performance liquid chromatography analysis. The structure of one of the prepared mapping ... -
Heparin Oligosaccharide Sequence and Size Essential for Inhibition of Pulmonary Artery Smooth Muscle Cell Proliferation
(2002-11-19)Heparin has a wide range of important biological activities including inhibition of pulmonary artery smooth muscle cell proliferation. To determine the minimum size of the heparin glycosaminoglycan chain essential for ... -
Heparin Oligosaccharides as Potential Therapeutic Agents in Senile Dementia
(Bentham Science, 2007-05-01)Heparin is a glycosaminoglycan mixture currently used in prophylaxis and treatment of thrombosis. Heparin possesses non-anticoagulant properties, including modulation of various proteases, interactions with fibroblast ... -
Heparin Oligosaccharides Bind P- and L-Selectin and Inhibit Acute Inflammation
(1993)Initial attachment of leukocytes to the vessel wall at sites of inflammation is supported by a family of carbohydrate-binding adhesion molecules called the selectins. Selectin ligands include sialyl-Lewis x (sLex, Neu5Ac ... -
Heparin oligosaccharides inhibit chemokine (C-X-C motif) ligand 12 (CXCL12) cardioprotection by binding orthogonal to the dimerization interface, promoting oligomerization, and competing with the chemokine (C-X-C motif) receptor 4 (CXCR4) amino-terminus
(The American Society for Biochemistry and Molecular Biology (ASBMB) and Elsevier, 2013)The ability to interact with cell surface glycosaminoglycans (GAGs) is essential to the cell migration properties of chemokines, but association with soluble GAGs induces the oligomerization of most chemokines including ...