Browsing The Linhardt Research Labs by Title

Now showing items 660-679 of 1022

    • N,N,N-trimethyl chitosan nanoparticles for the delivery of monoclonal antibodies against hepatocellular carcinoma cells 

      Vongchan, Preeyanat; Wutti-In, Yupanan; Sajomsang, Warayuth; Gonil, Pattarapond; Kothan, Suchart; Linhardt, Robert J. (Elsevier, 2011-04-22)
      N,N,N-Trimethyl chitosan chloride is capable of forming nanocomplexes with protein through ionotropic gelation. A monoclonal antibody, raised against human liver heparan sulfate proteoglycan and specifically inhibiting ...

    • N-glycans released from glycoproteins using a commercial kit and comprehensively analyzed with a hypothetical database 

      Zhang, Xing; Pagadala, Vijayakanth; Jester, Hannah M.; Lim, Andrew M.; Pham, Truong Quang; Goulas, Anna Marie P.; Liu, Jian; Linhardt, Robert J. (Royal Society of Chemistry, 2017)
      Heparan sulfate (HS) is a member of the glycosaminoglycans (GAG) family that plays essential roles in biological processes from animal sources. Heparin, a highly sulfated form of HS, is widely used as anticoagulant drug ...

    • N-glycolyl chondroitin synthesis using metabolically engineered E. coli 

      Awofiranye, Adeola E.; Baytas, Sultan N.; Xia, Ke; Badri, Abinaya; He, Wenqin; Varki, Ajit; Koffas, Mattheos; Linhardt, Robert J. (Springer, 2020)
      N-glycolyl chondroitin (Gc-CN) is a metabolite of N-glycolylneuraminic acid (Neu5Gc), a sialic acid that is commonly found in mammals, but not humans. Humans can incorporate exogenous Neu5Gc into their tissues from eating ...

    • N-sulfotestosteronan, a novel substrate for heparan sulfate 6-O-sulfotransferases and its analysis by oxidative degradation 

      Li, Guoyun; Masuko, Sayaka; Green, Dixy E.; Xu, Yongmei; Li, Lingyun; Zhang, Fuming; Xue, Changhu; Liu, Jian; Deangelis, Paul L.; Linhardt, Robert J. (Wiley, 2013-10-01)
      Testosteronan, an unusual glycosaminoglycan (GAG) first isolated from the microbe Comamonas testosteroni, was enzymatically synthesized in vitro by transferring uridine diphosphate sugars on β-p-nitrophenyl glucuronide ...

    • Nano-based Neoproteoglycans – Blood Compatible Carbon Nanotubes 

      Murugesan, Saravanababu; Park, Tae Joon; Yang, Hoichang; Mousa, Shaker; Linhardt, Robert J. (American Chemical Society (ACS), 2006-04-11)
      Although nanotechnology has provided a rich variety of nanomaterials (1–100 nm) for in vivo medical applications, the blood compatibility of all these nanobiomaterials is still largely unexamined. Here, we report the ...

    • Nanostructured glycan architecture is important in the inhibition of influenza A virus infection 

      Kwon, Seok Joon; Na, Dong Hee; Kwak, Jong Hwan; Douaisi, Marc; Zhang, Fuming; Park, Eun Ji; Park, Jong Hwan; Youn, Hana; Song, Chang Seon; Kane, Ravi S.; Dordick, Jonathan S.; Lee, Kyung Bok; Linhardt, Robert J. (2017-01-10)
      Rapid change and zoonotic transmission to humans have enhanced the virulence of the influenza A virus (IAV)3. Neutralizing antibodies fail to provide lasting protection from seasonal epidemics1,4. Furthermore, the effectiveness ...

    • Naringenin-Responsive Riboswitch-Based Fluorescent Biosensor Module for Escherichia coli Co-Cultures 

      Xiu, Yu; Jang, Sungho; Jones, J. Andrew; Zill, Nicholas A.; Linhardt, Robert J.; Yuan, Qipeng; Jung, Gyoo Yeol; Koffas, Mattheos A.G. (2017-10-01)
      The ability to design and construct combinatorial synthetic metabolic pathways has far exceeded our capacity for efficient screening and selection of the resulting microbial strains. The need for high-throughput rapid ...

    • Nature of the Interaction of Heparin with Acidic Fibroblast Growth Factor 

      Mach, Henryk; Volkin, David B.; Burke, Carl J.; Middaugh, C. Russell; Linhardt, Robert J.; Fromm, Jonathan R.; Loganathan, Duraikkannu; Mattsson, Lars (1993-01-01)
      The binding of human acidic fibroblast growth factor (aFGF) to heparin has been analyzed by a variety of different approaches to better elucidate the nature of this protein/sulfated polysaccharide interaction. Static and ...

    • Negative electron transfer dissociation Fourier transform mass spectrometry of glycosaminoglycan carbohydrates 

      Leach, Franklin E.; Wolff, Jeremy J.; Xiao, Zhongping; Ly, Mellisa; Laremore, Tatiana N.; Arungundram, Sailaja; Al-Mafraji, Kanar; Venot, Andre; Boons, Geert Jan; Linhardt, Robert J.; Amstera, I. Jonathan (Sage, 2011-01-01)
      Electron transfer through gas-phase ion–ion reactions has led to the widespread application ofelectron-based techniques once only capable in ion trapping mass spectrometers. Although anymass analyzer can, in theory, be ...

    • Negative electron transfer dissociation of glycosaminoglycans 

      Wolff, J.; Leach, F.; Laremore, T.; Kaplan, D.; Easterling, M.; Linhardt, Robert J.; Amster, J. (American Chemical Society (ACS), 2010)
      Structural characterization of glycosaminoglycans (GAGs) has been a challenge in the field of mass spectrometry, and the application of electron detachment dissociation (EDD) Fourier transform ion cyclotron resonance mass ...

    • Negative-Ion Fast Atom Bombardment Tandem Mass Spectrometry to Determine Sulfate and Linkage Position in Glycosaminoglycan-Derived Disaccharides 

      Lamb, Diane J.; Wang, Hui M.; Mallis, Larry M.; Linhardt, Robert J. (1992-01-01)
      Negative ion fast-atom bombardment tandem mass spectrometry has been used in the analysis of monosulfated. disaccharides. These commercially obtained disaccharides have been enzymatically prepared from glycosaminoglycans ...

    • Negative-ion mode capillary isoelectric focusing-mass spectrometry for charge-based separation of acidic oligosaccharides 

      Ouyang, Yilan; Han, Xiaorui; Xia, Qiangwei; Chen, Jianle; Velagapudi, Sheila; Xia, Ke; Zhang, Zhenqing; Linhardt, Robert J. (2019-01-02)
      Glycosaminoglycans (GAGs) are biologically and pharmacologically important linear, anionic polysaccharides containing various repeating disaccharides sequences. The analysis of these polysaccharides generally relies on ...

    • Neoproteoglycans in tissue engineering 

      Weyers, Amanda; Linhardt, Robert J. (FEBS Press, 2013-05-01)
      Proteoglycans, comprised of a core protein to which glycosaminoglycan chains are covalently linked, are an important structural and functional family of macromolecules found in the extracellular matrix. Advances in our ...

    • Neutralizing the anticoagulant activity of ultra-low molecular weight heparins using N-acetylglucosamine 6-sulfatase 

      Zhou, Xianxuan; Li, Lingyun; Linhardt, Robert J.; Liu, Jian (FEBS Press, 2013-05-01)
      Heparin has been the most commonly used anticoagulant drug for nearly a century. The drug heparin is generally categorized into three forms according to its molecular weight: unfractionated (UF, average molecular weight ...

    • New Approaches for Anticoagulation in Extracorporeal Therapy 

      Linhardt, Robert J.; Langer, Robert (1987-01-01)
      The need to fully heparinize patients undergoing extracorporeal therapy often leads to hemorrhagic complications. Two approaches have been used to solve this problem. The first involves full heparinization of blood entering ...

    • New Approaches for the Preparation of Hydrophobic Heparin Derivatives 

      Liu, Jian; Pervin, Azra; Gallo, Cindy M.; Desai, Umesh R.; Van Gorp, Cornelius L.; Linhardt, Robert J. (1994-01-01)
      A heparin derivative sufficiently lipophilic to be bound to plastics, forming blood-compatible supports, or to be used as an anticoagulant by transdermal or oral routes would be of great pharmaceutical interest. For such ...

    • New functional tools for anti-thrombogenic activity assessment of live surface glycocalyx 

      Dimitrievska, S.; Weyers, A.; Lin, T.; Cai, C.; Wu, W.; Tuggle, C.T.; Sundaram, S.; Balestrini, J.L.; Slattery, D.; Tchouta, L.; Kyriakides, T.R.; Tarbell, J.M.; Linhardt, Robert J.; Niklason, L.E. (American Heart Association (AHA) Journals and Wiley, 2016)
      Objective: It is widely accepted that the presence of a glycosaminoglycan-rich glycocalyx is essential for endothelialized vasculature health; in fact, a damaged or impaired glycocalyx has been demonstrated in many vascular ...

    • A New Glycosaminoglycan from the Giant African Snail Achatina fulica 

      Kim, Y.S.; Jo, Y.T.; Chang, I.M.; Toida, T.E.; Park, Y.; Linhardt, Robert J. (Elsevier, 1996)
      A new glycosaminoglycan has been isolated from the giant African snail Achatina fulica. This polysaccharide had a molecular weight of 29,000, calculated based on the viscometry, and a uniform repeating disaccharide structure ...

    • New insights into the action of bacterial chondroitinase AC I and hyaluronidase on hyaluronic acid 

      Tao, Lei; Song, Fei; Xu, Naiyu; Li, Duxin; Linhardt, Robert J.; Zhang, Zhenqing (2017-02-20)
      Hyaluronic acid (HA), a glycosaminoglycan, is a linear polysaccharide with negative charge, composed of a repeating disaccharide unit [→4)-β-d-glucopyranosyluronic acid (1→3)-β-d- N-acetyl-d-glucoaminopyranose (1→]n ([→4) ...

    • New Insights into the Heparan Sulfate Proteoglycan binding Activity of Apolipoprotein E4 

      Peters-Libeu, C.; Lund-Katz, S.; Phillips, M.; Wehrli, S.; Hernaiz, M.J.; Capila, I.; Linhardt, Robert J.; Raffai, R.; Newhouse, Y.M.; Zhou, M.F.; Weisgraber, K.H. (2001)
      Defective binding of apolipoprotein E (apoE) to heparan sulfate proteoglycans (HSPGs) is associated with increased risk of atherosclerosis due to inefficient clearance of lipoprotein remnants by the liver. The interaction ...