Browsing The Linhardt Research Labs by Title
Now showing items 693-712 of 1022
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Oligosaccharide Compositional Analysis of Heparin and Low Molecular Weight Heparins by Capillary Electrophoresis
(1993)The application of capillary electrophoresis to total compositional analysis of heparin and low-molecular-weight heparin samples has been studied. Optimum resolution of 17 defined oligosaccharides was obtained with the ... -
Oligosaccharide Mapping of Low Molecular Weight Heparins: Structural Differences and their Relationship to Activity
(1990)Low molecular weight heparins from a variety of commercial sources were examined. These had been prepared by several methods including peroxidative cleavage, nitrous acid cleavage, chemical beta-elimination, enzymatic ... -
On-line CE-LIF-MS analysis of glycans labeled with teal fluorescence dye using electrokinetic sheath liquid pump-based nanospray ion source
(2018)Rationale: N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics ... -
On-line separation and characterization of hyaluronan oligosaccharides derived from radical depolymerization
(Elsevier, 2013-01-01)Hydroxyl radicals are widely implicated in the oxidation of carbohydrates in biological and industrial processes and are often responsible for their structural modification resulting in functional damage. In this study, ... -
One-Pot Enzymatic Synthesis of Heparin from N-Sulfoheparosan
(2022-01-01)Heparin, a glycosaminoglycan-based anticoagulant drug, is prepared as an extract of animal tissues. Heparosan, an Escherichia coli (E. coli) K5 capsular polysaccharide with the structure →4)-β-D-glucuronic acid (1 → ... -
Online capillary zone electrophoresis negative electron transfer dissociation tandem mass spectrometry of glycosaminoglycan mixtures
(Elsevier, 2019-11-01)Glycosaminoglycans (GAGs) are important biological molecules that are highly anionic and occur in nature as complex mixtures. A platform that combines capillary zone electrophoresis (CZE) separations with mass spectrometry ... -
Optimization of bioprocess conditions improves production of a CHO cell-derived, bioengineered heparin
(Wiley, 2015-07-01)Heparin is the most widely used anticoagulant drug in the world today. Heparin is currently produced from animal tissues, primarily porcine intestines. A recent contamination crisis motivated development of a non-animal-derived ... -
Optimization of germination and ultrasonic-assisted extraction for the enhancement of γ-aminobutyric acid in pumpkin seed
(Wiley, 2022-01-01)Germination and ultrasonic-assisted extraction (UAE) are economical and effective methods to enhance bioactive compounds in plant seeds. We optimized the germination parameters and UAE parameters by using response surface ... -
Oral administration of fucosylated chondroitin sulfate oligomers in gastro-resistant microcapsules exhibits a safe antithrombotic activity
(2021-01-01)Fucosylated chondroitin sulfate (FCS) polysaccharide isolated from sea cucumber has potent anticoagulant activity. Based on its resistance to the enzymes present in vertebrates, it may serve as an anticoagulant and shows ... -
Oral fate and stabilization technologies of lactoferrin: a systematic review
(2021-01-01)Lactoferrin (Lf), a bioactive protein initially found in many biological secretions including milk, is regarded as the nutritional supplement or therapeutic ligand due to its multiple functions. Research on its mode of ... -
Order out of complexity; protein structures that interact with heparin
(2001-09-01)Many proteins of widely differing functionality and structure are capable of binding heparin. Structural characterisations of the many types of such complexes are being reported in ever-increasing number and at improved ... -
Orthogonal analytical approaches to detect potential contaminants in heparin
(2009-10-06)Heparin is a widely used anticoagulant and antithrombotic agent. Recently, a contaminant, oversulfated chondroitin sulfate (OSCS), was discovered within heparin preparations. The presence of OSCS within heparin likely led ... -
Oversulfated chondroitin sulfate interaction with heparin-binding proteins: New insights into adverse reactions from contaminated heparins
(Elsevier, 2009-08-01)An oversulfated chondroitin sulfate (OSCS) was identified as a contaminant to pharmaceutical heparin and severe anaphylactoid reactions were ascribed to this contaminant. An examination of the biochemistry underlying both ... -
Oversulfated Chondroitin Sulfate is a major contaminant in Heparin associated with Adverse Clinical Events
(Nature, 2008)Recently, certain lots of heparin have been associated with an acute, rapid onset of serious side effects indicative of an allergic-type reaction. To identify potential causes for this sudden rise in side effects, we ... -
Oversulfated Chondroitin Sulfate: Impact of a Heparin Impurity, Associated with Adverse Clinical Events, on Low Molecular Weight Heparin Preparation
(American Chemical Society (ACS), 2008-09-25)Heparin, a widely used anticoagulant, is being rapidly displaced by low-molecular-weight heparins. Recently, certain lots of heparin have been associated with anaphylactoid-type reactions resulting from contamination with ... -
Ozonolysis of the double bond of the unsaturated uronate residue in low molecular weight heparin and K5 heparosan
(Elsevier, 2011-01-01)Ozone is known to add across and cleave carbon-carbon double bonds. Ozonolysis is widely used for the preparation of pharmaceuticals, for bleaching substances and for killing microorganisms in air and water sources. Some ... -
Parameters Affecting the Efficiency of Affinity-Based Reversed Micellar Extraction and Separation
(1997-07-01)Parameters affecting the efficiency of affinity-based reversed micellar extraction and separation (ARMES) in glycoprotein purification -
Parent heparin and daughter LMW heparin correlation analysis using LC-MS and NMR
(2017-04-08)Heparin is a structurally complex, polysaccharide anticoagulant derived from livestock, primarily porcine intestinal tissues. Low molecular weight (LMW) heparins are derived through the controlled partial depolymerization ... -
Partial depolymerization of pectin by photochemical reaction
(2010-06-16)Complex heterogeneous polysaccharides that comprise pectin were partially depolymerized by a photochemical reaction using ultraviolet light in the presence of titanium dioxide catalyst. In a period of 6h at pH 7, this ... -
Patents related to Dengue Virus Infection
(2002-08-01)This review discusses patents relevant to dengue virus infection. Dengue virus is a mosquito-transmitted flavivirus that usually causes an acute self-limited febrile illness, but may result in more severe life threatening ...