Browsing The Linhardt Research Labs by Title
Now showing items 835-854 of 1022
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Search for the Heparin Antithrombin III Binding Site Precursor
(Elsevier, 1992)The last step of heparin biosynthesis is thought to involve the action of 3-O-sulfotransferase resulting in the formation of an antithrombin III (ATIII) binding site required for heparin's anticoagulant activity. The ... -
Search for the Heparin Antithrombin III Binding Site Precursor
(Elsevier, 1992)The last step of heparin biosynthesis is thought to involve the action of 3-O-sulfotransferase resulting in the formation of an antithrombin III (ATIII) binding site required for heparin's anticoagulant activity. The ... -
Selective N-Sulfation of Glucosamine Derivatives Using Phenyl Chlorosulfate in Non-Aqueous Solvent
(1996-01-01)The selective N-sulfation of 2-amino-2-deoxy-D-glucopyranose derivatives having unprotected hydroxyl groups with phenyl chlorosulfate and triethylamine in anhydrous organic solvent followed by addition of aqueous sodium ... -
Selective, switchable fluorescent probe for heparin based on aggregation-induced emission
(2016-12-01)A positively charged tetraphenylethene (TPE) derivative, TPE-4MN, was synthesized as a probe for heparin based on aggregation induced emission. On the addition of 5.0 μg/mL of heparin, TPE-4MN showed an enhanced emission ... -
Self-assembly of carbon nanotube films from room temperature ionic liquids
(2013-07-01)A novel method for the assembly of thin, uniform transparent and conductive films of single-walled carbon nanotubes (SWCNTs) on glass substrates is described. This process involves the initial transfer of a temporary ... -
Semi-rational evolution of pyruvate carboxylase from Rhizopus oryzae for elevated fumaric acid synthesis in Saccharomyces cerevisiae
(2022-01-01)Dicarboxylic acids are widely used in food, pharmaceutical, and chemical industries. Pyruvate carboxylase (PYC) plays a pivotal role in the production of dicarboxylic acids in microbial fermentation process. Our previous ... -
Semi-synthesis of chondroitin sulfate-E from chondroitin sulfate-A
(Elsevier, 2012-01-04)Chondroitin sulfate-E (chondroitin-4, 6-disulfate) was prepared from chondroitin sulfate-A (chondroitin-4 - sulfate) by regioselective sulfonation, performed using trimethylamine sulfur trioxide in formamide under argon. ... -
Sensitive Cells: Enabling Tools for Static and Dynamic Control of Microbial Metabolic Pathways
(2015-12-01)Natural metabolic pathways are dynamically regulated at the transcriptional, translational, and protein levels. Despite this, traditional pathway engineering has relied on static control strategies to engender changes in ... -
Separation and Purification of Carbohydrates
(2001)The separation and purification of carbohydrates is a broad field encompassing molecules having substantially different physical properties (see Chap. 1.2) requiring a wide variety of analytical and separation methods. ... -
Separation of Glycosaminoglycan Derived Oligosaccharides by Capillary Electrophoresis Using Reverse Polarity
(1994-08-15)A comparative study on compositional analysis of two sets of eight unsaturated disaccharide standards derived from heparin/heparan sulfate and chondroitin/dermatan sulfate was carried out using capillary electrophoresis ... -
Separation of Hydroxyl-Protected Heparin Derived Disaccharides using Reversed-Phase High Performance Liquid Chromatography
(1995-06-30)A simple and efficient method for the separation of hydrophobic derivatives of glycosaminoglycan-derived disaccharides is described. Hydroxyl-protected derivatives of a trisulfated disaccharide, prepared from heparin using ... -
Sequence analysis and domain motifs in the decorin glycosaminoglycan chain
(The American Society for Biochemistry and Molecular Biology (ASBMB) and Elsevier, 2013-10-11)Decorin proteoglycan is comprised of a core protein containing a single O-linked dermatan sulfate/chondroitin sulfate glycosaminoglycan (GAG) chain. Although the sequence of the decorin core protein is determined by the ... -
Sequence Analysis of Highly Sulfated Heparin-Derived Oligosaccharides Using Fast Atom Bombardment Mass Spectrometry
(1989-01-01)Heparin, a polydisperse, sulfated copolymer of 1----4 linked glucosamine and uronic acid residues, has been used clinically as an anticoagulant for half a century. Despite a yearly use of over 50 million doses in the U.S. ... -
Sequence analysis of native oligosaccharides using negative ESI tandem MS
(Bentham Science, 2009-07-01)Carbohydrates exhibit many physiologically and pharmacologically important activities, yet their complicated structure and sequence pose major analytical challenges. Although their structural complexity makes analysis of ... -
Sequencing analysis of β-glucan from highland barley with high performance anion exchange chromatography coupled to quadrupole time–of–flight mass spectrometry
(2017-12-01)Highland barley is one of the hull-less barleys that contains high amounts of β-glucan. A detailed sequence analysis is needed to support the application of the β-glucan from highland barley. The β-conformation and 1 → 3/1 ... -
Sequencing of 3-O-Sulfate Containing Heparin Decasaccharides with a Partial Antithrombin III Binding Site
(2000-09-12)Heparin- and heparan sulfate-like glycosaminoglycans (HLGAGs) represent an important class of molecules that interact with and modulate the activity of growth factors, enzymes, and morphogens. Of the many biological functions ... -
Sequencing the dermatan sulfate chain of decorin
(American Chemical Society (ACS), 2017-11-22)Glycomics represents one of the last frontiers and most challenging in omic analysis. Glycosylation occurs in the endoplasmic reticulum and the Golgi organelle and its control is neither well-understood nor predictable ... -
Sequencing the Oligosaccharide Pool in Dalteparin Sodium with Offline Strong Anion Exchange (SAX)-HPLC and ESI-MS/MS
(2018)Low molecular weight heparins (LMWHs) are widely used anticoagulant drugs. The composition and sequence of LMWH oligosaccharides determine their safety and efficacy. The short oligosaccharide pool in LMWHs undergoes more ... -
Sialic Acid Donors: Chemical Synthesis and Glycosylation
(2004)Sialic acids (or ulosonic acids) are a family of acidic ketoses (including neuraminic acid, KDN and KDO) that are found at the non-reducing terminus of many glycoconjugates. These saccharide residues are recognized ligands ... -
Signal amplification by Glyco-qPCR for ultrasensitive detection of carbohydrates: Applications in glycobiology
(Wiley, 2012-11-19)Tiny amounts of carbohydrates (ca. 1 zmol) can be detected quantitatively by a real-time method based on the conjugation of carbohydrates with DNA markers (see picture). The proposed method (glyco-qPCR) provides uniform, ...