Browsing Linhardt Research Labs Papers by Title
Now showing items 7-26 of 1022
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A Colorimetric Assay for Chemical Heparin in Plasma
(1982-07-15)A simple, rapid colorimetric assay for plasma heparin is presented. The assay employs the metachromasia of azure A when heparin is added. It is useful for 0 to 10 units/ml and does not depend on heparin's anticoagulant ... -
A comparative secretome analysis of industrial Aspergillus oryzae and its spontaneous mutant ZJGS-LZ-21
(2017-05-02)Aspergillus oryzae koji plays a crucial role in fermented food products due to the hydrolytic activities of secreted enzymes. In the present study, we performed a comparative secretome analysis of the industrial strain of ... -
A Dose Escalation Study or Org 10172 (Low Molecular Weight Heparinoid) in Stroke
(1989-01-01)An intravenous infusion of a low molecular weight heparinoid, with a reduced risk of hemorrhage, may be an alternative to heparin in the management of acute ischemic stroke. To evaluate this hypothesis, we studied the ... -
A Flexible Carbon/Sulfur-Cellulose Core-Shell Structure for Advanced Lithium–Sulfur Batteries
(2018-11-01)Lithium sulfur (Li-S) batteries are viewed as a promising candidate for next-generation energy storage systems due to their high energy density, low cost and ease of manufacturing. However, rapid capacity decay caused by ... -
A fucoidan from sea cucumber Pearsonothuria graeffei with well-repeated structure alleviated gut microbiota dysbiosis and metabolic syndromes in HFD-fed mice
(2018)A high-fat diet (HFD) has been a major contributor to increasing morbidity caused by metabolic syndromes. Functional foods from natural sources are potential choices for addressing metabolic diseases because they provide ... -
A General Method for the Stereospecific Synthesis of C-glycosidesof Ulosonic Acids by Samarium-Mediated Reductive Dechlorination
(1998)C-glycosylation reactions were performed with the 2-chloroderivatives of NANA, KDN and KDO. C-glycosides were obtainedwhen carbonyl compounds (ketone or sugar aldehyde) were coupled tothe 2-chloro-derivatives ... -
A Highly Stable Covalent Conjugated Heparin Biochip for Heparin-Protein Interactions Studies
(2002-05-15)Heparin is a proteoglycan composed of highly sulfated linear polysaccharides of alternating uronic acid and glucosamine that interacts with a wide variety of proteins and peptides (1). Heparin and the structurally related ... -
A Kinetic Model for FGF, FGFR and Proteoglycan Signal Transduction Complex Assembly
(2004)The current working model for fibroblast growth factor receptor (FGFR) dimerization and activation requires the assembly of a ternary complex of fibroblast growth factor (FGF), FGFR, and heparin or heparan sulfate proteoglycan ... -
A Molecular Hero Suit for In Vitro and In Vivo DNA Nanostructures
(2019-06-26)Precise control of DNA base pairing has rapidly developed into a field full of diverse nanoscale structures and devices that are capable of automation, performing molecular analyses, mimicking enzymatic cascades, biosensing, ... -
A mutant cell library for systematic analysis of heparan sulfate
(Nature, 2018-11-01)Heparan sulfate (HS) is a complex linear polysaccharide that modulates a wide range of biological functions. Elucidating the structure–function relationship of HS has been challenging. Here we report the generation of an ... -
A New Method for Sequencing Linear Oligosaccharides on Gels Using Charged, Fluorescent Conjugates
(1991-08-18)A new method is described for sequencing linear oligosaccharides on gels using charged, fluorescent conjugates. The reducing ends of various mono-, di-, tri-, and tetra-saccharides were conjugated with monopotassium ... -
A Novel Fabrication of Poly (e-caprolactone)Microspheres from Blends of Poly (e-caprolactone)and Poly (ethylene glycol) s
(1999)A novel method for the preparation of solvent-free poly(ε-caprolactone) (PCL) microspheres from PCL/poly(ethylene glycol) (PEG) blends was developed and demonstrated. The particle size of the PCL spheres are in the range ... -
A novel laminin-binding protein mediates microbial-endothelial cell interactions and facilitates dissemination of Lyme disease pathogens
(2020-04-07)Borrelia burgdorferi conserved gene products BB0406 and BB0405, members of a common B. burgdorferi paralogous gene family, share 59% similarity. Although both gene products can function as potential porins, only BB0405 is ... -
A novel route for double-layered encapsulation of probiotics with improved viability under adverse conditions
(2020-04-25)To improve the survivability of probiotics under the harsh conditions, a novel double-layered vehicle, which was developed by a one-step coaxial electrospinning procedure, was here used to encapsulate the probiotics. The ... -
A novel structural fucosylated chondroitin sulfate from Holothuria mexicana and its effects on growth factors binding and anticoagulation
(2018-02-01)Fucosylated chondroitin sulfate (FCS), a structurally distinct glycosaminoglycan from the body wall of sea cucumber, possesses many biological properties and pharmacology functions. The refined structure of FCS isolated ... -
A purification process for heparin and precursor polysaccharides using the pH responsive behavior of chitosan
(Wiley, 2015-09-01)The contamination crisis of 2008 has brought to light several risks associated with use of animal tissue derived heparin. Because the total chemical synthesis of heparin is not feasible, a bioengineered approach has been ... -
A rolling circle amplification based platform for ultrasensitive detection of heparin
(2021-01-21)Heparin has a variety of pharmacological uses, including applications for anti-tumor metastasis, anti-inflammatory and anti-viral activities and is widely used as a clinical anticoagulant. Due to its widespread applications ... -
A Sensitive Method for the Quantification of b-Glucuronidase Activity in Urine Using Capillary Electrophoresis with Fluorescence Detection
(1998)Capillary electrophoresis (CE) with fluorescence detection was used to determine the concentration of 4-methylumbelliferone liberated from 4-methylumbelliferyl-beta-D-glucuronide by beta-glucuronidase. Enzyme substrate ... -
A simple strategy for the separation and purification of water-soluble polysaccharides from the fresh Spirulina platensis
(2017-02-11)A novel and industrially applicable strategy for separating and purifying the water-soluble polysaccharides from the fresh Spirulina platensis is introduced in this study. After hot water extraction, using chitosan ... -
A structural analysis of glycosaminoglycans from lethal and non-lethal breast cancer tissues: Towards a novel class of theragnostics for personalized medicine in oncology
(2012)Cancer is one of the leading noncommunicable diseases that vastly impacts both developed and developing countries. Truly innovative diagnostics that inform disease susceptibility, prognosis, and/or response to treatment ...