Browsing Linhardt Research Labs Papers by Title
Now showing items 302-321 of 1022
-
E. coli K5 fermentation and the preparation of heparosan, a bioengineered heparin precursor
(Wiley, 2010-12-15)Heparosan is an acidic polysaccharide natural product, which serves as the critical precursor in heparin biosynthesis and in the chemoenzymatic synthesis of bioengineered heparin. Heparosan is also the capsular polysaccharide ... -
E. coli K5 heparosan fermentation and improvement by genetic engineering
(2011)N-acetyl heparosan is the precursor for the biosynthesis of the important anticoagulant drug heparin. The E. coli K5 capsular heparosan polysaccharide provides a promising precursor for in vitro chemoenzymatic production ... -
Effect of a variety of carbon nanotubes on the iodide-triiodide redox couple
(2013)The iodide (I−)-triiodide () redox pair was used as a model system to evaluate the potential catalytic activity of various carbon nanotubes. Aqueous solutions of hydroiodic acid were irradiated with ultraviolet light in ... -
Effect of Carboxyl-Reduced Heparin on the Growth Inhibition of Bovine Pulmonary Artery Smooth Muscle Cells
(Elsevier, 2010-06-16)Heparin (HP) inhibits the proliferation of bovine pulmonary artery smooth muscle cells (BPASMC's), among other cell types in vitro. In order to develop a potential therapeutic agent to reverse vascular remodeling, we are ... -
Effect of electrical stimulation conditions on neural stem cells differentiation on cross-linked PEDOT: PSS films
(Frontiers Media SA, 2021-02-12)The ability to culture and differentiate neural stem cells (NSCs) to generate functional neural populations is attracting increasing attention due to its potential to enable cell-therapies to treat neurodegenerative diseases. ... -
Effect of eliminase gene (elmA) deletion on heparosan production and shedding in Escherichia coli K5
(Elsevier, 2013)Escherichia coli K5 produces heparosan and sheds it into the growth medium in a temperature dependent manner. The shedding is believed to be controlled, at least in part, by enzyme action on the cell-associated capsular ... -
Effect of Fully Sulfated Glycosaminoglycans on Pulmonary ArterySmooth Muscle Cell Proliferation
(1999)Fully sulfated heparin and other glycosaminoglycans, namely heparan, chondroitin, and dermatan sulfates, and hyaluronan have been prepared by using sulfur trioxide under mild chemical conditions. All these derivatives were ... -
Effect of Genomic Integration Location on Heterologous Protein Expression and Metabolic Engineering in E. coli
(2017-04-21)Chromosomal integration offers a selection-free alternative to DNA plasmids for expression of foreign proteins and metabolic pathways. Episomal plasmid DNA is convenient but has drawbacks including increased metabolic ... -
Effect of Heparin, Heparin Fragments, and Cortisone on Cerebral Endothelial Cell Growth In Vitro and In Vivo
(1986)Heparin and heparin fragments in combination with corticosteroids have been shown to markedly inhibit tumor angiogenesis. Experiments were performed to test the hypothesis that heparin, heparin fragments, and the combination ... -
Effect of high aspect ratio filler on dielectric properties of polymer composites: A study on barium titanate fibers and graphene platelets
(2012-06-01)High aspect ratio fillers are predicted to increase the dielectric constant of polymer composites more efficiently than spherical fillers according to the rule of mixtures. Using high aspect ratio fillers is a promising ... -
Effect of high glucose on glycosaminoglycans in cultured retinal endothelial cells and rat retina
(2022)Introduction The endothelial glycocalyx regulates vascular permeability, inflammation, and coagulation, and acts as a mechanosensor. The loss of glycocalyx can cause endothelial injury and contribute to several microvascular ... -
Effect of simultaneous steam explosion and alkaline depolymerization of corncob lignin and cellulose structure
(Croatian Society of Chemical Engineers, 2018-07-01)Simultaneous steam explosion and alkaline depolymerization was studied to develop an economic and green process for alkaline pretreatment of lignocellulose. Steam explosion facilitated alkaline delignification and decreased ... -
Effect of sophorolipid n-alkyl ester chain length on its interfacial properties at the almond oil-water interface
(2016-06-07)Sophorolipids (SLs), produced by Candida bombicola, are of interest as potential replacements for hazardous commercial surfactants. For the first time, a series of molecularly edited SLs with ethyl (EE), n-hexyl (HE), and ... -
The effect of surface modification of aligned poly-L-lactic acid electrospun fibers on fiber degradation and neurite extension
(Public Library of Science (PLoS), 2015-09-04)The surface of aligned, electrospun poly-L-lactic acid (PLLA) fibers was chemically modified to determine if surface chemistry and hydrophilicity could improve neurite extension from chick dorsal root ganglia. Specifically, ... -
Effect of the sulfation pattern of sea cucumber-derived fucoidan oligosaccharides on modulating metabolic syndromes and gut microbiota dysbiosis caused by HFD in mice
(2019-04-01)Fucoidans from sea cucumbers are potential functional food ingredients with a well-defined repeating structure. However, there have been fewer function studies of fucoidan oligosaccharides. In the present study, we first ... -
Effect of Very Low Molecular Weight Heparin-Derived Oligosaccharides on Lipoprotein Lipase Release in Rabbits
(1986)Oligosaccharide fragments of heparin were prepared using flavobacterial heparinase. Following sizing, these oligosaccharide fractions were administered (i.v.) to rabbits and were examined for their ability to release ... -
Effective Inhibition of SARS-CoV-2 Entry by Heparin and Enoxaparin derivatives
(American Society for Microbiology, 2021-02-01)Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has caused a pandemic of historic proportions and continues to spread globally, with enormous consequences to human health. Currently there is no vaccine, ... -
Effects of glycosaminoglycan supplementation in the chondrogenic differentiation of bone marrow- and synovial- derived mesenchymal stem/stromal cells on 3D-extruded poly (ε-caprolactone) scaffolds
(2021)The lack of effective and long-term treatments for articular cartilage defects has increased the interest for innovative tissue engineering strategies. Such approaches, combining cells, biomaterial matrices and external ... -
Efficient, environmentally-friendly and specific valorization of lignin: Promising role of non-radical lignolytic enzymes
(2017-06-01)Lignin is the second most abundant bio-resource in nature. It is increasingly important to convert lignin into high value-added chemicals to accelerate the development of the lignocellulose biorefinery. Over the past several ... -
Electrical Stimulation of Neural-Differentiating iPSCs on Coaxial Nanofibers
(2021-08-07)Neural tissue engineering strategies are paramount to create fully mature neurons, necessary for new therapeutic strategies for neurological diseases or the creation of reliable in vitro models. Scaffolds can provide ...