Browsing Linhardt Research Labs Papers by Title
Now showing items 137-156 of 1022
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Cell-based microscale isolation of glycoaminoglycans for glycomics study
(Taylor and Francis, 2012-07-09)Glycomics research requires the isolation of glycans from cells for structural characterization and functional studies of the glycans. A method for cell-based microscale isolation and quantification of highly sulfated, ... -
Cell-Bound IL-8 Increases in Bronchial Epithelial Cells after Arylsulfatase B Silencing due to Sequestration with Chondroitin-4-Sulfate
(2010-01-01)The chemokine IL-8 is critically important in inflammatory processes in human tissues, and IL-8 interactions with sulfated glycosaminoglycans have been implicated in modification of inflammatory responses in bronchial ... -
Cellular Binding of Hepatitis C Virus Envelope Glycoprotein E2 Requires Cell Surface Heparan Sulfate
(Elsevier, 2003-10-17)The conservation of positively charged residues in the N terminus of the hepatitis C virus (HCV) envelope glycoprotein E2 suggests an interaction of the viral envelope with cell surface glycosaminoglycans. Using recombinant ... -
Certain High Molecular Weight Heparin Chains Have Affinity for Vitronectin
(2001-07-15)Vitronectin is a 70-kDa protein that is found in both the extracellular matrix as well as serum. Vitronectin is one of the few proteins that regulates both the complement and the coagulation systems. Heparin is known to ... -
Challenges of pectic polysaccharides as a prebiotic from the perspective of fermentation characteristics and anti-colitis activity
(2021-10-15)Several studies are described that contribute to the systematic exploration of new aspects of digestion, fermentation, and biological activities of pectic polysaccharides (PPS) leading to a better understanding of prebiotics. ... -
Changes in composition and sulfation patterns of glycoaminoglycans in renal cell carcinoma
(2016-02-01)Glycosaminoglycans (GAGs) are heterogeneous, linear, highly charged, anionic polysaccharides consisting of repeating disaccharides units. GAGs have some biological significance in cancer progression (invasion and metastasis) ... -
Changes in cultured endothelial cell glycosaminoglycans under hyperglycemic conditions and the effect of insulin and heparin
(Springer Nature, 2009-08-20)Background: Heparan sulfate proteoglycans (HSPGs) contain glycosaminoglycan (GAG) chains made primarily of heparan sulfate (HS). Hyperglycemia in diabetes leads to endothelial injury and nephropathy, retinopathy and ... -
Changes in glycosaminoglycan structure on differentiation of human embryonic stem cells towards mesoderm and endoderm lineages
(Elsevier, 2014-01-01)Background: Proteoglycans are found on the cell surface and in the extracellular matrix, and serve as prime sites for interaction with signaling molecules. Proteoglycans help regulate pathways that control stem cell fate, ... -
Characteristics of global organic matrix in normal and pimpled chicken eggshells
(Elsevier, 2017-01-01)The organic matrix from normal and pimpled calcified chicken eggshells were dissociated into acid-insoluble, water-insoluble, and facultative-soluble (both acid- and water-soluble) components, to understand the influence ... -
Characteristics of glycosaminoglycans in chicken eggshells and the influence of disaccharide composition on eggshell properties
(Elsevier, 2016-01-01)Glycosaminoglycans (GAG) are linear, highly negatively charged polysaccharides that may perform an important role in biomineralization. GAG were isolated from chicken eggshell membranes and calcified shells. Disaccharide ... -
Characterization and application of a putative transcription factor SUT2 in Pichia pastoris
(2020-09-01)Pichia pastoris is able to metabolize methanol via a specific MUT (methanol utilization) pathway. Based on the powerful AOX1 (Alcohol Oxidase 1) promoter, the P. pastoris expression system has become one of the most widely ... -
Characterization and comparative analysis of toxin–antitoxin systems in Acetobacter pasteurianus
(2019-06-01)Bacterial toxin-antitoxin (TA) systems play important roles in diverse cellular regulatory processes. Here, we characterize three putative type II TA candidates from Acetobacter pasteurianus and investigate the profile of ... -
Characterization of a Heparan Sulfate Octasaccharide that Binds to Herpes Simplex Viral Type 1 Glycoprotein D
(Elsevier, 2002)Herpes simplex virus type 1 utilizes cell surface heparan sulfate as receptors to infect target cells. The unique heparan sulfate saccharide sequence offers the binding site for viral envelope proteins and plays critical ... -
Characterization of a new monoclonal anti-glypican-3 antibody specific for the hepatocellular carcinoma cell line, HepG2
(2017-01-01)AIM: To characterize the antigen on HepG2 cell that is specifically recognized by a new monoclonal antibody raised against human liver heparan sulfate proteoglycan (HSPG), clone 1E4-1D9. METHODS: The antigen recognized by ... -
Characterization of Bacteroides Species from Human Intestine that Degrades Glycosaminoglycans
(NRC CNRC, 1998)Polysaccharide lyases that can degrade glycosaminoglycans (GAGs) were identified in an anaerobic strain living in the human intestine. The strain was isolated from the stool of a healthy male and identified as Bacteroides ... -
Characterization of Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures and the Cortex of Neonatal Rats
(Springer, 2021-03-01)Astrocytes are major producers of the extracellular matrix (ECM), which is involved in the plasticity of the developing brain. In utero alcohol exposure alters neuronal plasticity. Glycosaminoglycans (GAGs) are a family ... -
Characterization of Glycosaminoglycans by Capillary Electrophoresis
(2003-01-01)Glycosaminoglycans are linear, sulfated polysaccharides that are found in virtually all animal tissues. With the exception of hyaluronic acid, glycosaminoglycans are biosynthesized as proteoglycans in which one or more ... -
Characterization of Heparan Sulfate from the Unossified Antler of Cervus elaphus
(2005-02-28)The antler is the most rapidly growing tissue in the animal kingdom. According to previous reports, antler glycosaminoglycans (GAGs) consist of all kinds GAGs except for heparan sulfate (HS). Chondroitin sulfate is the ... -
Characterization of heparin and severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) spike glycoprotein binding interactions
(Elsevier, 2020)Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has resulted in a pandemic and continues to spread around the globe at an unprecedented rate. To date, no effective therapeutic is available to fight its ... -
Characterization of Heparin Binding by a Peptide from Amyloid P Component Using Capillary Electrophoresis, Surface Plasmon Resonance, and Isothermal Titration Calorimetry
(2002-07-22)Synthetic peptides based on amino-acid residues 27-38 of human serum amyloid P component represent a novel type of heparin binders as they do not contain clusters of basic amino acids or other known features associated ...