Browsing Linhardt Research Labs Papers by Subject "Chemical and biological engineering"
Now showing items 21-40 of 1020
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A novel structural fucosylated chondroitin sulfate from Holothuria mexicana and its effects on growth factors binding and anticoagulation
(2018-02-01)Fucosylated chondroitin sulfate (FCS), a structurally distinct glycosaminoglycan from the body wall of sea cucumber, possesses many biological properties and pharmacology functions. The refined structure of FCS isolated ... -
A purification process for heparin and precursor polysaccharides using the pH responsive behavior of chitosan
(Wiley, 2015-09-01)The contamination crisis of 2008 has brought to light several risks associated with use of animal tissue derived heparin. Because the total chemical synthesis of heparin is not feasible, a bioengineered approach has been ... -
A rolling circle amplification based platform for ultrasensitive detection of heparin
(2021-01-21)Heparin has a variety of pharmacological uses, including applications for anti-tumor metastasis, anti-inflammatory and anti-viral activities and is widely used as a clinical anticoagulant. Due to its widespread applications ... -
A Sensitive Method for the Quantification of b-Glucuronidase Activity in Urine Using Capillary Electrophoresis with Fluorescence Detection
(1998)Capillary electrophoresis (CE) with fluorescence detection was used to determine the concentration of 4-methylumbelliferone liberated from 4-methylumbelliferyl-beta-D-glucuronide by beta-glucuronidase. Enzyme substrate ... -
A simple strategy for the separation and purification of water-soluble polysaccharides from the fresh Spirulina platensis
(2017-02-11)A novel and industrially applicable strategy for separating and purifying the water-soluble polysaccharides from the fresh Spirulina platensis is introduced in this study. After hot water extraction, using chitosan ... -
A structural analysis of glycosaminoglycans from lethal and non-lethal breast cancer tissues: Towards a novel class of theragnostics for personalized medicine in oncology
(2012)Cancer is one of the leading noncommunicable diseases that vastly impacts both developed and developing countries. Truly innovative diagnostics that inform disease susceptibility, prognosis, and/or response to treatment ... -
A System for Heparin Removal
(1982)Extracorporeal medical machines rely on systemic heparinization to improve blood compatibility. However, heparin can lead to serious complications such as hemorrhaging. We propose a new approach to control heparin levels ... -
Abnormally high content of free glucosamine residues identified in a preparation of commercially available porcine intestinal heparan sulfate
(American Chemical Society (ACS), 2016-07-05)Heparan sulfate (HS) polysaccharides are ubiquitous in animal tissues as components of proteoglycans, and they participate in many important biological processes. HS carbohydrate chains are complex and can contain rare ... -
Accelerated Degradation of Poly(e-caprolactone) by Organic Amines
(1994)The solid-state degradation of poly(epsilon-caprolactone) catalyzed by primary, secondary and tertiary alkylamines was investigated. The degradation process was monitored by weight loss and molecular weight change measured ... -
Accelerated Heparin Stability Studies
(1996-01-01)The objective of this study was to extend our understanding of the stability of heparin. Sodium heparin, derived from porcine intestinal mucosa, was first incubated in 0.1 N hydrochloric acid and 0.1 N sodium hydroxide at ... -
Acceptor Specificity of the Pasteurella Hyaluronan and Chondroitin Synthases and Production of Chimeric Glycosaminoglycans
(Elsevier, 2007-01-05)The hyaluronan (HA) synthase, PmHAS, and the chondroitin synthase, PmCS, from the Gram-negative bacterium Pasteurella multocida polymerize the glycosaminoglycan (GAG) sugar chains HA or chondroitin, respectively. The ... -
Action Pattern of Polysaccharide Lyases on Glycosaminoglycans
(1994-06-01)The action pattern of polysaccharide lyases on glycosaminoglycan substrates was examined using viscosimetric measurements and gradient polyacrylamide gel electrophoresis (PAGE). Heparin lyase I (heparinase, EC 4.2.2.7) and ... -
Active Site of Chondroitin AC Lyase Revealed by the Structure of Enzyme-Oligosaccharide Complexes and Mutagenesis
(2001-02-27)The crystal structures of Flavobacterium heparinium chondroitin AC lyase (chondroitinase AC; EC 4.2.2.5) bound to dermatan sulfate hexasaccharide (DShexa), tetrasaccharide (DStetra), and hyaluronic acid tetrasaccharide ... -
Additional role of nicotinic acid hydroxylase for the transformation of 3-succinoyl-pyridine by Pseudomonas sp. JY-Q
(2021-03-01)Nicotine and nicotinic acid (NA) are both considered to be representatives of N-heterocyclic aromatic compounds, and their degradation pathways have been revealed in Pseudomonas species. However, the cooccurrence of these ... -
Addressing endotoxin issues in bioengineered heparin
(2012-11-01)Heparin is a widely used clinical anticoagulant that is prepared from pig intestine. A contamination of heparin in 2008 has led to a reexamination of animal-derived pharmaceuticals. A bioengineered heparin prepared by ... -
Adsorption of doxorubicin on poly(methyl methacrylate)−chitosan−heparin-coated activated carbon beads
(2012)Extracorporeal filter cartridges, filled with an activated carbon bead (ACB) adsorbent, have been used for removal of overdosed cancer drugs from the blood. Coatings on adsorbent matrices, poly(methyl methacrylate) ... -
Advances in preparation and synthesis of heparin and related products
(Elsevier, 2020-12-01)Heparin is a naturally occurring glycosaminoglycan from livestock, principally porcine intestine, and is clinically used as an anticoagulant drug. A limitation to heparin production is that it depends on a single animal ... -
Affinity Capillary Electrophoresis Employing Immobilized Glycosaminoglycanto Resolve Heparin-Binding Peptides
(1998)A new capillary electrophoresis technique has been developed for the affinity resolution of synthetic heparin-binding peptides using an immobilized glycosaminoglycan. Heparin and heparan sulfate were immobilized onto fused ... -
Affinity Chromatography Using Enzymatically Synthesized Nucleotide-ContainingDNA Binding Polymers
(1999)A series of nucleotide-containing polyphenols has been synthesized by a simple, two-step enzymatic method. The binding properties of these synthetic polymers to complementary oligonucleotides have been evaluated using a ... -
Affinity Purification of Secreted Alkaline Phosphatase Produced by the Baculovirus Expression Vector System
(2001-01-01)Human secreted alkaline phosphatase (SEAP) was produced in a stably-transformed Spodoptera frugiperda Sf-9 insect cell line (Sfb4GalT) following infection with a recombinant Autographa californica multiple nuclear ...