Browsing Linhardt Research Labs Papers by Title
Now showing items 229-248 of 1022
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Conformational Flexibility of Heparinases from PL12 family: Crystal Structure and Substrate Specificity of Heparinase III from Bacteroides thetaiotaomicron
(2017)Glycosaminoglycans (GAGs) are linear polysaccharides comprised of disaccharide repeat units, a hexuronic acid, glucuronic acid or iduronic acid, linked to a hexosamine, N-acetylglucosamine (GlcNAc) or N-acetylgalactosamine. ... -
Conformational Study of D 4-UronateMonosaccharide and Glycosaminoglycan-derived Disaccharide
(1998)Sixteen delta 4-uronate monosaccharides were chemically synthesized. Their carboxy group was protected as a methyl or benzyl ester, the anomeric hydroxyl group as a benzyl glycoside and the 2 and 3 hydroxyl groups were ... -
Conjugation of ginsenoside Rg3 with gold nanoparticles
(2011-01-20)Ginsenoside Rg3 was reported to have important biological activities. We demonstrate conjugation and quantification procedures of ginsenoside Rg3 to gold nanoparticles for future biological and medical applications. ... -
Construction and Characterization of Heparan Sulfate Heptasaccharide Microarray
(2017-01-01)A targeted heptasaccharide library was synthesised to prepare a heparan sulphate (HS) microarray. The array was probed with two glycan-binding proteins, HS 3-O-sulphotransferase 1 and antithrombin, demonstrating the binding ... -
Construction and functional characterization of truncated versions of recombinant keratanase II from Bacillus circulans
(2017-10-01)There is a need for degradative enzymes in the study of glycosaminoglycans. Many of these enzymes are currently available either in their natural or recombinant forms. Unfortunately, progress in structure-activity studies ... -
Construction of heparan sulfate microarray for investigating the binding of specific saccharide sequences to proteins
(2021-03-01)Heparan sulfate (HS) is a heterogeneous, extracellular glycan that interacts with proteins and other molecules affecting many biological processes. The specific binding motifs of HS interactions are of interest, but have ... -
Control of the heparosan N-deacetylation leads to an improved bioengineered heparin
(Springer, 2011-07-01)The production of the anticoagulant drug heparin from non-animal sources has a number of advantages over the current commercial production of heparin. These advantages include better source material availability, improved ... -
Controlled Photochemical Depolymerization of K5 Heparosan, a Bioengineered Heparin Precursor
(Elsevier, 2011-08-30)Heparosan is a polysaccharide, which serves as the critical precursor in heparin biosynthesis and chemoenzymatic synthesis of bioengineered heparin. Because the molecular weight of microbial heparosan is considerably larger ... -
Controlled Release of Protein and Vaccines from Poly(Ester) Microspheres In Vitro
(1991)Biodegradable microspheres of poly(L-lactide) and copolymers of lactide and glycolide have been prepared by spray drying. Degradation studies of these microspheres using residual mass measurements, viscometry and gel ... -
Controlled Release of Protein and Vaccines from Poly(Ester) Microspheres In Vitro
(1991)Biodegradable microspheres of poly(L-lactide) and copolymers of lactide and glycolide have been prepared by spray drying. Degradation studies of these microspheres using residual mass measurements, viscometry and gel ... -
Copper regulates the interactions of antimicrobial piscidin peptides from fish mast cells with formyl peptide receptors and heparin
(Elsevier, 2018-10-05)Phagocytic cells in fish secrete antimicrobial peptides (AMPs) such as piscidins, glycosaminoglycans such as heparin, and copper ions as first-line immune defenses. Recently, we established that Cu2+ coordination by piscidins ... -
Coupling liquid chromatography and tandem mass spectrometry to electrophoresis for in-depth analysis of glycosaminoglycan drugs: heparin and the multicomponent sulodexide
(2021-01-26)Glycosaminoglycans (GAGs) contribute to the treatment of many human diseases, especially in the field of thrombosis, because of their anticoagulant activity. GAGs interrupt the coagulation process by interacting with ... -
CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR Arrays for dCas9-Mediated Multiplex Transcriptional Repression in E. coli
(2015-03-30)Programmable control over an addressable global regulator would enable simultaneous repression of multiple genes and would have tremendous impact on the field of synthetic biology. It has recently been established that ... -
CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production
(Springer Nature, 2017-01-17)Anthocyanins are a class of brightly colored, glycosylated flavonoid pigments that imbue their flower and fruit host tissues with hues of predominantly red, orange, purple, and blue. Although all anthocyanins exhibit ... -
Crystal Structure and Mutational Analysis of Heparan Sulfate 3-O-Sulfotransferase Isoform 1
(Elsevier, 2004-06-11)Heparan sulfate interacts with antithrombin, a protease inhibitor, to regulate blood coagulation. Heparan sulfate 3-O-sulfotransferase isoform 1 performs the crucial last step modification in the biosynthesis of anticoagulant ... -
Crystal Structure of a Ternary FGF-FGFR-Heparin Complex Reveals a Dual Role for Heparin in FGFR Binding and Dimerization
(2000-01-01)The crystal structure of a dimeric 2:2:2 FGF:FGFR:heparin ternary complex at 3 A resolution has been determined. Within each 1:1 FGF:FGFR complex, heparin makes numerous contacts with both FGF and FGFR, thereby augmenting ... -
Crystal Structure of Chondroitinase B from Flavobacterium heparinumand its Complex with a Disaccharide Product at 1.7 ÅResolution
(1999)Glycosaminoglycans (GAGs) are a family of acidic heteropolysaccharides, including such molecules as chondroitin sulfate, dermatan sulfate, heparin and keratan sulfate. Cleavage of the O-glycosidic bond within GAGs can be ... -
Crystallographic Analysis of Calcium-Dependent Heparin Binding to Annexin A2
(American Society for Biochemistry and Molecular Biology (ASBMB) and Elsevier, 2006-10-20)Annexin A2 and heparin bind to one another with high affinity and in a calcium-dependent manner, an interaction that may play a role in mediating fibrinolysis. In this study, three heparin-derived oligosaccharides of ... -
CS Lyases: Structure, Activity and Applications in Analysis and the Treatment of Diseases
(Elsevier, 2006-12-01)Glycosaminoglycans (GAGs) are a family of highly sulfated, complex mixture of linear polysaccharides that display a wide array of biological activities (Boneu, 1996; Jackson et al., 1991). GAGs can be classified into four ... -
Cultivation of fractionated cells from a bioactive-alkaloid-bearing marine sponge Axinella sp
(2021-05-01)Sponges are among the most primitive multicellular organisms and well-known as a major source of marine natural products. Cultivation of sponge cells has long been an attractive topic due to the prominent evolutionary and ...