Browsing Linhardt Research Labs Papers by Title
Now showing items 269-288 of 1022
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Determination of the pKa of Glucuronic Acid and Heparin's Carboxyl Groups by 13C NMR
(1991)As part of our continuing studies on heparin, the present paper uses 13C-n.m.r. spectroscopy to examine the acidity of heparin's uronic acid carboxylate groups. Heparin contains three different uronic acids. In porcine ... -
Determination of the Structure of Oligosaccharides Prepared from Acharan Sulfate
(1998-01-01)The fine structure of acharan sulfate, a recently discovered glycosaminoglycan isolated from Achatina fulica , was examined. This glycosaminoglycan has a major disaccharide repeating unit of -->4)-alpha-D-GlcNpAc(1-->4)- ... -
Development of a method to analyze the complexes of enoxaparin and platelet factor 4 with size-exclusion chromatography
(2019-02-05)Heparin, a highly sulfated glycosaminoglycan, has been used as a clinical anticoagulant over 80 years. However, heparin-induced thrombocytopenia and thrombosis (HITT) is a serious side effect of heparin therapy, resulting ... -
Development of hydrophilic interaction chromatography with quadruple-time-of-flight mass spectrometry for heparin and low molecular weight heparin disaccharide analysis
(2016-01-30)Rationale: Heparin and low molecular weight heparin (LMWH) are widely used as clinical anticoagulants. The determination of their composition and structural heterogeneity still challenges analysts. Methods: Disaccharide ... -
Development of low molecular weight heparin by H2O2/ascorbic acid with ultrasonic power and its anti-metastasis property
(2019-07-15)Low molecular weight heparins (LMWHs) are currently used as an anticoagulant agent since unfractionated heparin (UFH) can cause serious adverse drug reactions. LMWHs are commercially prepared using different methods such ... -
Dialkyl Imidazolium Benzoates-- Room Temperature Ionic Liquids Useful in the Peracetylation and Perbenzoylation of Simple and Sulfated Saccharides
(2003-01-01)Dialkyl imidazolium benzoates, room temp. ionic liqs. including 1-ethyl-3-Me imidazolium benzoate, 1-butyl-3-Me imidazolium benzoate and 1-hexyl-3-Me imidazolium benzoate were used in the peracetylation and perbenzoylation ... -
Diastereocontrolled Synthesis of Carbon Glycosides of N-Acetylneuraminic Acid via Glycosyl Samarium(III) Intermediates
(1997)α-O-Glycosides of N-acetylneuraminic acid (Neu5Ac, 1, Scheme 1) are often found terminating the oligosaccharide component of cell-surface glycoproteins and glycolipids. Neu5Ac is involved in a number of important biological ... -
Dietary pectic substances enhance gut health by its poly-component: A review
(2021)Pectic substances, one of the cell wall polysaccharides, exist widespread in vegetables and fruits. A surge of recent research has revealed that pectic substances can inhibit gut inflammation and relieve inflammatory bowel ... -
Differences in the Interaction of Heparin with Arginine and Lysine and the Importance of Basic Amino Acids in the Binding of Heparin to aFGF
(1995)Although the interaction of proteins with glycosaminoglycans (GAGs) such as heparin are of great importance, the general structural requirements for protein- or peptide-GAG interaction have not been well characterized. ... -
Differential Anticoagulant Activity of Heparin Fragments Prepared Using Microbial Heparinase
(Elsevier, 1982)Heparin of an average molecular weight of 13,000 with known polydispersity was degraded using microbial heparinase. The kinetics of this degradation were followed by four assays which measured the anticoagulant activity ... -
Differential effect of homologous NicR2A/NicR2Bs and endogenous ectopic strong promoters on nicotine metabolism in Pseudomonas sp. JY-Q
(2021)Nicotine is a toxic environmental pollutant that widely exists in tobacco wastes. As a natural nicotine-degrading strain, Pseudomonas sp. strain JY-Q still has difficulties degrading high concentrations of nicotine. In ... -
Differential Interactions of Heparin and Heparan Sulfate Glycosaminoglycans with the Selectins. Implications for the Use of Unfractionated and LowMolecular Weight Heparins as Therapeutic Agents
(1998)The selectins are calcium-dependent C-type lectins that bind certain sialylated, fucosylated, sulfated glycoprotein ligands. L-selectin also recognizes endothelial proteoglycans in a calcium-dependent manner, via heparan ... -
Differentiating chondroitin sulfate glycosaminoglycans using collision-induced dissociation; uronic acid cross-ring diagnostic fragments in a single stage of tandem mass spectrometry
(Sage, 2015-06-07)The stereochemistry of the hexuronic acid residues of the structure of glycosaminoglycans (GAGs) is a key feature that affects their interactions with proteins and other biological functions. Electron-based tandem mass ... -
Digestibility of squash polysaccharide under simulated salivary, gastric and intestinal conditions and its impact on short chain fatty acid production in type-2 diabetic rats
(2020-05-01)In vitro digestive conditions were simulated to investigate the digestibility of polysaccharides prepared from squash (SPS). A small amount of free glucose monosaccharide was released after salivary and intestinal digestion ... -
Dimerization Interface of Osteoprotegerin Revealed by Hydrogen-deuterium Exchange Mass Spectrometry
(Elsevier, 2018-01-01)Previous structural studies of osteoprotegerin (OPG), a crucial negative regulator of bone remodeling and osteoclastogenesis, were mostly limited to the N-terminal ligand-binding domains. It is now known that the three ... -
Direct Measurement of the Interaction of Glycosaminoglycans and a Heparin Decasaccharide with Malaria Circumsporozoite Protein
(2001-09-25)Circumsporozoite (CS) protein is a predominant surface antigen of malaria sporozoites, the infective form of the parasite, and has been used for making anti-malaria vaccines. For the first time we have examined the interaction ... -
Directional Immobilization of Heparin onto the Nonporous Surface of Polystyrene Microplates
(Future Science, 1997)Heparin, a sulfated polysaccharide,exhibits anticoagulant activity mediat-ed through the binding of a specificpentasaccharide sequence in its struc-ture to the protein antithrombin III, acoagulation protease ... -
Directional Immobilization of Heparin to Beaded Supports
(1994-01-01)Heparin was immobilized in a defined orientation on Sepharose, agarose, and polyacrylamide supports by coupling through its reducing end. This is expected to mimic the attachment of heparin to the protein core in the ... -
Disaccharide analysis of glycosaminoglycan mixtures by ultra-performance liquid chromatography-mass spectrometry
(Elsevier, 2012)Glycosaminoglycans are a family of polysaccharides widely distributed in all eukaryotic cells. These polyanionic, linear chain polysaccharides are composed of repeating disaccharide units that are often differentially ... -
Disaccharide Compositional Analysis of Heparin and Heparan Sulfate Using Capillary Zone Electrophoresis
(1991-01-01)Capillary zone electrophoresis (CZE) was used to separate eight commercial disaccharide standards of the structure delta UA2X(1----4)-D-GlcNY6X (where delta UA is 4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid, GlcN ...