Browsing Linhardt Research Labs Papers by Title
Now showing items 369-388 of 1022
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Examination of glycosaminoglycan binding sites on the XCL1 dimer
(American Chemical Society (ACS), 2016-03-01)Known for its distinct metamorphic behavior, XCL1 interconverts between a canonical chemokine folded monomer (XCL1mon) that interacts with the receptor, XCR1, and a unique dimer (XCL1dim) that interacts with glycosaminoglycans ... -
Examination of the Substrate Specificity of Heparin and Heparan Sulfate Lyases
(1990-03-01)We have examined the activities of different preparations of heparin and heparan sulfate lyases from Flavobacterium heparinum. The enzymes were incubated with oligosaccharides of known size and sequence and with complex ... -
Existing cell wall fragments modify the thermal properties and hydrolysis of potato starch
(2018-12-01)The effect of cell wall fragments on the thermal properties and hydrolysis of potato starch were investigated. The relative degree of crystallinity of purified starch and starch with cell wall fragments were 19.7% and ... -
Expanding Glycosaminoglycan Chemical Space: Towards the Creation of Sulfated Analogs, and Novel and Chimeric Polymers
(2017-07-01)Glycosaminoglycans (GAGs) have therapeutic potential in areas ranging from angiogenesis, inflammation, hemostasis and cancer. GAG bioactivity is conferred by intrinsic structural features, such as disaccharide composition, ... -
Expanding the chemical space of polyketides through structure-guided mutagenesis of Vitis vinifera stilbene synthase
(2015-06-11)Several natural polyketides (PKs) have been associated with important pharmaceutical properties. Type III polyketide synthases (PKS) that generate aromatic PK polyketides have been studied extensively for their substrate ... -
Expedient synthesis of core disaccharide building blocks from natural polysaccharides for heparan sulfate oligosaccharide assembly
(Wiley, 2019-12-16)The complex sulfation motifs of heparan sulfate glycosaminoglycans (HS GAGs) play critical roles in many important biological processes. However, an understanding of their specific functions has been hampered by an inability ... -
Exploration of the Action Pattern of Streptomyces HyaluronateLyase Using High Resolution Capillary Electrophoresis
(1997)Hyaluronic acid was treated exhaustively with a hyaluronate lyase (hyaluronidase, EC 4.2.2.1) from Streptomyces hyalurolyticus to obtain a tetrasaccharide and a hexasaccharide product in a molar ratio of 1 to 1.2. The ... -
Exposure to common food additive carrageenan leads to reduced sulfatase activity and increase in sulfated glycosaminoglycans in human epithelial cells
(Elsevier, 2012-06-01)The commonly used food additive carrageenan, including lambda (λ), kappa (κ) and iota (ι) forms, is composed of galactose disaccharides linked in alpha-1,3 and beta-1,4 glycosidic bonds with up to three sulfate groups per ... -
Expression and functional identification of two homologous nicotine dehydrogenases, NicA2 and Nox, from Pseudomonas sp. JY-Q
(2021-02-01)Nicotine contamination in tobacco waste effluent (TWE) from tobacco industry is a serious threat to public health and environment. Microbial degradation is an impending approach to remove nicotine and transform it into ... -
Expression and secretion of glycosylated heparin biosynthetic enzymes using Komagataella pastoris
(2017-04-01)Heparin, an anticoagulant drug, is biosynthesized in selected animal cells. The heparin biosynthetic enzymes mainly consist of sulfotransferases and all are integral transmembrane glycoproteins. These enzymes are generally ... -
Expression of C1 Esterase Inhibitor by the Baculovirus Expression System: Preparation, Purification and Characterization
(2001-01-01)C1 esterase inhibitor (C1INH) is an important regulator of the classical complement pathway. Hereditary deficiency of C1INH causes angioedema of the skin, gut, and respiratory tissues that may be fatal. C1INH replacement ... -
Expression of chondroitin-4-O-sulfotransferase in Escherichia coli and Pichia pastoris
(2017-09-01)Chondroitin sulfates are linear sulfated polysaccharides called glycosaminoglycans. They are important nutraceutical and pharmaceutical products that are biosynthesized through the action of chondroitin sulfotransferases ... -
Expression of human liver HSPGs on myeloid leukemia
(Elsevier, 2007-02-01)Heparan sulfate proteoglycans (HSPGs) play important biological roles in cell–matrix adhesion processes and are essential regulators of growth actions. The expression of the different HSPGs in itself is tightly regulated ... -
Expression of low endotoxin 3-O-sulfotransferase in Bacillus subtilis
(2013-10-01)A key enzyme for the biosynthesis and bioengineering of heparin, 3-O-sulfotransferase-1 (3-OST-1), was expressed and purified in Gram-positive Bacillus subtilis and Bacillus megaterium. Western blotting, protein sequence ... -
Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach
(2020-07-01)The clinical demand for tissue-engineered bone is growing due to the increase of non-union fractures and delayed healing in an aging population. Herein, we present a method combining additive manufacturing (AM) techniques ... -
Extraction and characterization of RG-I enriched pectic polysaccharides from mandarin citrus peel
(Elsevier, 2018-06-01)Pectin is extensively used as thickener and gelling agent in the food industry. Commercial pectin is usually comprised of homogalacturonans (HG) with small amount of rhamnogalacturonan-I (RG-I). However, recent studies ... -
Extraction Methods Affect the Structure and Bioactivity of Goji (Lycium barbarum) Polysaccharides
(Multidisciplinary Digital Publishing Institute (MDPI), 2020)Polysaccharides are considered to be the most important active substances in Goji. However, the structure of polysaccharides varies according to the extraction methods applied, and the solution used to prepare Goji ... -
Extraction temperature is a decisive factor for the properties of a pectin
(2021-03-01)Pectin is an important food thickener and gelling agent and also, based on its bioactivities, is becoming viewed as a healthy polysaccharide. The functional properties of pectin are related to its structure which, if raw ... -
Extraction, structure and bioactivities of the polysaccharides from Pleurotus eryngii: A Review
(2020-05-01)Pleurotus eryngii (also known as king trumpet mushroom or king oyster mushroom) is an edible mushroom cultivated widely in many regions of the world. Polysaccharides from P. eryngii have a variety of biological activities, ... -
Extruded bioreactor perfusion culture supports the chondrogenic differentiation of human mesenchymal stem/stromal cells in 3D porous poly (ε-caprolactone) scaffolds
(2019)Novel bioengineering strategies for the ex vivo fabrication of native-like tissue-engineered cartilage are crucial for the translation of these approaches to clinically manage highly prevalent and debilitating joint diseases. ...