Browsing Linhardt Research Labs Papers by Title
Now showing items 428-447 of 1022
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Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices using liquid chromatography-tandem mass spectrometry
(2020-01-01)Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, ... -
Glycosaminoglycan remodeling during chondrogenic differentiation of human bone marrow−/synovial-derived mesenchymal stem/stromal cells under normoxia and hypoxia
(2020-06-01)Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators ... -
Glycosaminoglycanomics of cultured cells using a rapid and sensitive LC-MS/MS approach
(2015-05-15)Glycosaminoglycans (GAGs), a family of polysaccharides widely distributed in eukaryotic cells, are responsible for a wide array of biological functions. Quantitative disaccharide compositional analysis is one of the primary ... -
Glycosaminoglycans and glycolipids as potential biomarkers in lung cancer
(2017-10-01)In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched ... -
Glycosaminoglycans Can Influence Fibroblast Growth Factor-2 Mitogenicity Without Significant Growth Factor Binding
(1998-01-06)Fibroblast growth factors are important heparin binding, mitogenic proteins. The binding site in heparin and heparan sulfate for fibroblast growth factor-2 (basic fibroblast growth factor) has been described as rich in ... -
Glycosaminoglycans compositional analysis of urodele axolotl (Ambystoma mexicanum) and porcine retina
(2019)Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide. Humans cannot regenerate retina, however, axolotl (Ambystoma mexicanum), ... -
Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II
(Springer, 2018-02-01)Glycosaminoglycans (GAGs) play an important role in stabilizing the gel state of eye vitreous humour. In this study, the composition of GAGs present in bovine eye vitreous was characterized through disaccharide analysis ... -
Glycosaminoglycans from earthworms (Eisenia andrei
(Springer, 2010)The whole tissue of the earthworm (Eisenia andrei) was lyophilized and extracted to purify glycosaminoglycans. Fractions, eluting from an anion-exchange column at 1.0 M and 2.0 M NaCl, showed the presence of acidic ... -
Glycosaminoglycans from fish swim bladder: isolation, structural characterization and bioactive potential
(2018-02-01)The swim bladder of fish is an internal gas-filled organ that allows fish to control their buoyancy and swimming depth. Fish maws (the dried swim bladders of fish) have been used over many centuries as traditional medicines, ... -
Glycosaminoglycans in human cerebrospinal fluid determined by LC-MS/MS MRM
(Elsevier, 2019-02-15)Glycosaminoglycans (GAGs) were recovered from human cerebral spinal fluid (CSF) and after their conversion to disaccharides using polysaccharide lyases were analyzed by liquid chromatography tandem mass spectrometry using ... -
Glycosaminoglycans in infectious disease
(Wiley, 2013)Glycosaminoglycans (GAGs) are complex carbohydrates that are ubiquitously present on the cell surface and in the extracellular matrix. Interactions between GAGs and pathogens represent the first line of contact between ... -
Glycosaminoglycans of the chicken muscular stomach or gizzard
(2017-02-01)Glycosaminoglycans (GAGs) were prepared from the muscular stomach or gizzard of the chicken. The content of GAGs on a dry weight basis contains 0.4 wt.% a typical value observed for a muscle tissue. The major GAG components ... -
Glycosaminoglycans of the porcine central nervous system
(American Chemical Society (ACS), 2010-11-16)Glycosaminoglycans (GAG) are known to participate in central nervous system processes such as development, cell migration, and neurite outgrowth. In this paper, we report an initial glycomics study on GAGs from porcine ... -
Glycosylation in Room Temperature Ionic Liquid using Unprotected and Unactivated Donors
(Elsevier, 2007-02-26)Glycosylation in room temperature ionic liquid is demonstrated using unprotected and unactivated donors. Modest yields of simple benzyl glycosides and disaccharides of glucose, mannose and N-acetylgalactosamine were obtained ... -
Gold and silver nanoparticles conjugated with heparin-derivative possess anti-angiogenesis properties
(2009-11-09)Silver and gold nanoparticles display unique physical and biological properties that have been extensively studied for biological and medical applications. Typically, gold and silver nanoparticles are prepared by chemical ... -
Gradient Polyacrylamide Gel Electrophoresis for Determination of the Molecular Weight of Heparin and Low Molecular Weight Heparin Derivatives
(1992-01-01)The M(r) values of pharmaceutical heparins and low-molecular-weight (LMW) heparin derivatives were examined as part of a collaborative study to develop methods for their characterization. Standard methods of M(r) determination ... -
GRASP depletion-mediated Golgi fragmentation impairs glycosaminoglycan synthesis, sulfation, and secretion
(Springer, 2022-04-01)Synthesis of glycosaminoglycans, such as heparan sulfate (HS) and chondroitin sulfate (CS), occurs in the lumen of the Golgi, but the relationship between Golgi structural integrity and glycosaminoglycan synthesis is not ... -
Green glycosylation using ionic liquid to prepare alkyl glycosides for studying carbohydrate-protein interactions by SPR
(2009-03-09)Several simple glycosides of D-glucose (Glc) and N-acetyl-D-galactosamine (GalNAc) were prepared in a single step glycosylation reaction using unprotected and unactivated sugar donors. The resulting GalNAc glycoside, ... -
Green recovery of pectic polysaccharides from citrus canning processing water
(2017-02-15)Canned citrus segments are popularly consumed worldwide with a large global market. However, the production needs chemical treatment to remove segments membranes, producing a large amount of acidic and basic effluents with ... -
Green solvents in carbohydrate chemistry: from raw materials to fine chemicals
(2015-07-22)In recent decades, there has been an increasing effort to reduce the use of fossil fuels and oil derivatives, to decrease environmental pollution, and to counteract global warming. The use of biomass as raw material is ...