Browsing Linhardt Research Labs Papers by Title

Now showing items 435-454 of 1022

    • Glycosaminoglycans from earthworms (Eisenia andrei 

      Im, A.R.; Park, Y.; Sim, J.S.; Zhang, Z.; Liu, Z.; Linhardt, Robert J.; Kim, Y.S. (Springer, 2010)
      The whole tissue of the earthworm (Eisenia andrei) was lyophilized and extracted to purify glycosaminoglycans. Fractions, eluting from an anion-exchange column at 1.0 M and 2.0 M NaCl, showed the presence of acidic ...

    • Glycosaminoglycans from fish swim bladder: isolation, structural characterization and bioactive potential 

      Pan, Yongxi; Wang, Peipei; Zhang, Fuming; Yu, Yanlei; Zhang, Xing; Lin, Lei; Linhardt, Robert J. (2018-02-01)
      The swim bladder of fish is an internal gas-filled organ that allows fish to control their buoyancy and swimming depth. Fish maws (the dried swim bladders of fish) have been used over many centuries as traditional medicines, ...

    • Glycosaminoglycans in human cerebrospinal fluid determined by LC-MS/MS MRM 

      Yu, Yanlei; Zhang, Fuming; Colón, Wilfredo; Linhardt, Robert J.; Xia, Ke (Elsevier, 2019-02-15)
      Glycosaminoglycans (GAGs) were recovered from human cerebral spinal fluid (CSF) and after their conversion to disaccharides using polysaccharide lyases were analyzed by liquid chromatography tandem mass spectrometry using ...

    • Glycosaminoglycans in infectious disease 

      Kamhi, E.; Joo, E.J.; Dordick, J.S.; Linhardt, Robert J. (Wiley, 2013)
      Glycosaminoglycans (GAGs) are complex carbohydrates that are ubiquitously present on the cell surface and in the extracellular matrix. Interactions between GAGs and pathogens represent the first line of contact between ...

    • Glycosaminoglycans of the chicken muscular stomach or gizzard 

      Chen, Yin; Reddy, Megan; Yu, Yanlei; Zhang, Fuming; Linhardt, Robert J. (2017-02-01)
      Glycosaminoglycans (GAGs) were prepared from the muscular stomach or gizzard of the chicken. The content of GAGs on a dry weight basis contains 0.4 wt.% a typical value observed for a muscle tissue. The major GAG components ...

    • Glycosaminoglycans of the porcine central nervous system 

      Liu, Zhenling; Masuko, Sayaka; Solakyildirim, Kemal; Pu, Dennis; Linhardt, Robert J.; Zhang, Fuming (American Chemical Society (ACS), 2010-11-16)
      Glycosaminoglycans (GAG) are known to participate in central nervous system processes such as development, cell migration, and neurite outgrowth. In this paper, we report an initial glycomics study on GAGs from porcine ...

    • Glycosylation in Room Temperature Ionic Liquid using Unprotected and Unactivated Donors 

      Park, Tae Joon; Weiwer, Michel; Yuan, Xuejun; Baytas, Sultan N.; Munoz, Eva M.; Murugesan, Saravanababu; Linhardt, Robert J. (Elsevier, 2007-02-26)
      Glycosylation in room temperature ionic liquid is demonstrated using unprotected and unactivated donors. Modest yields of simple benzyl glycosides and disaccharides of glucose, mannose and N-acetylgalactosamine were obtained ...

    • Gold and silver nanoparticles conjugated with heparin-derivative possess anti-angiogenesis properties 

      Kemp, Melissa M.; Kumar, Ashavani; Mousa, Shaymaa; Dyskin, Evgeny; Yalcin, Murat; Ajayan, Pulickel; Linhardt, Robert J.; Mousa, Shaker A. (2009-11-09)
      Silver and gold nanoparticles display unique physical and biological properties that have been extensively studied for biological and medical applications. Typically, gold and silver nanoparticles are prepared by chemical ...

    • Gradient Polyacrylamide Gel Electrophoresis for Determination of the Molecular Weight of Heparin and Low Molecular Weight Heparin Derivatives 

      Edens, R.E.; Al-Hakim, A.; Weiler, J.M.; Rethwisch, D.G.; Fareed, J.; Linhardt, Robert J. (1992-01-01)
      The M(r) values of pharmaceutical heparins and low-molecular-weight (LMW) heparin derivatives were examined as part of a collaborative study to develop methods for their characterization. Standard methods of M(r) determination ...

    • GRASP depletion-mediated Golgi fragmentation impairs glycosaminoglycan synthesis, sulfation, and secretion 

      Ahat, Erpan; Song, Yuefan; Xia, Ke; Reid, Whitney; Li, Jie; Bui, Sarah; Zhang, Fuming; Linhardt, Robert J.; Wang, Yanzhuang (Springer, 2022-04-01)
      Synthesis of glycosaminoglycans, such as heparan sulfate (HS) and chondroitin sulfate (CS), occurs in the lumen of the Golgi, but the relationship between Golgi structural integrity and glycosaminoglycan synthesis is not ...

    • Green glycosylation using ionic liquid to prepare alkyl glycosides for studying carbohydrate-protein interactions by SPR 

      Muñoz, F.J.; Pérez, J.; André, S.; Gabius, H.J.; Sinisterra, J.V.; Hernáiz, M.J.; Linhardt, Robert J. (2009-03-09)
      Several simple glycosides of D-glucose (Glc) and N-acetyl-D-galactosamine (GalNAc) were prepared in a single step glycosylation reaction using unprotected and unactivated sugar donors. The resulting GalNAc glycoside, ...

    • Green recovery of pectic polysaccharides from citrus canning processing water 

      Chen, Jianle; Cheng, Huan; Wu, Dan; Linhardt, Robert J.; Zhi, Zijian; Yan, Lufeng; Chen, Shiguo; Ye, Xingqian (2017-02-15)
      Canned citrus segments are popularly consumed worldwide with a large global market. However, the production needs chemical treatment to remove segments membranes, producing a large amount of acidic and basic effluents with ...

    • Green solvents in carbohydrate chemistry: from raw materials to fine chemicals 

      Farrán, A.; Cai, C.; Sandoval, M.; Xu, Y.; Liu, J.; Hernáiz, M.J.; Linhardt, Robert J. (2015-07-22)
      In recent decades, there has been an increasing effort to reduce the use of fossil fuels and oil derivatives, to decrease environmental pollution, and to counteract global warming. The use of biomass as raw material is ...

    • HClO4-SiO2 Catalyzed Glycosylation using Sugar Trichloroacetimidates as Glycosyl Donors 

      Du, Yuguo; Wei, Guohua; Cheng, Shuihong; Hua, Yuxia; Linhardt, Robert J. (2006-01-16)
      Silica-supported perchloric acid (HClO4–SiO2) has been used as an efficient promoter, as a replacement of TMSOTf, in various glycosylation reactions using sugar trichloroacetimidates as glycosyl donors. Operational simplicity, ...

    • Heavy chain transfer by tumor-necrosis-factor-stimulated-gene-6 to the bikunin proteoglycan 

      Lamkin, Elliott; Cheng, Georgiana; Calabro, Anthony; Hascall, Vincent C.; Joo, Eun Ji; Li, Lingyun; Linhardt, Robert J.; Lauer, Mark E. (The American Society for Biochemistry and Molecular Biology (ASBMB) and Elsevier, 2015-02-20)
      We present data that hyaluronan (HA) polysaccharides, about 14-86 monosaccharides in length, are capable of accepting only a single heavy chain (HC) from inter-α-inhibitor via transfer by tumor necrosis factor-stimulated ...

    • Heavy heparin: A stable isotope-enriched, chemoenzymatically-synthesized, poly-component drug 

      Cress, Brady F.; Bhaskar, Ujjwal; Vaidyanathan, Deepika; Williams, Asher; Cai, Chao; Liu, Xinyue; Fu, Li; M-Chari, Vandhana; Zhang, Fuming; Mousa, Shaker A.; Dordick, Jonathan S.; Koffas, Mattheos A.G.; Linhardt, Robert J. (Wiley, 2019-04-23)
      Heparin is a highly sulfated, complex polysaccharide and widely used anticoagulant pharmaceutical. In this work, we chemoenzymatically synthesized perdeuteroheparin from biosynthetically enriched heparosan precursor obtained ...

    • Heparan Sulfate Domains Required for Fibroblast Growth Factor 1 and 2 Signaling through Fibroblast Growth Factor Receptor 1c 

      Schultz, Victor; Suflita, Mathew; Liu, Xinyue; Zhang, Xing; Yu, Yanlei; Li, Lingyun; Green, Dixy E.; Xu, Yongmei; Zhang, Fuming; DeAngelis, Paul L.; Liu, Jian; Linhardt, Robert J. (The American Society for Biochemistry and Molecular Biology (ASBMB) and Elsevier, 2017-02-10)
      A small library of well defined heparan sulfate (HS) polysaccharides was chemoenzymatically synthesized and used for a detailed structure-activity study of fibroblast growth factor (FGF) 1 and FGF2 signaling through FGF ...

    • Heparan sulfate facilitates spike protein-mediated SARS-Cov-2 host cell invasion and contributes to increased infection of SARS-Cov-2 G614 mutant and in lung cancer 

      Yue, Jingwen; Jin, Weihua; Yang, Hua; Faulkner, John; Song, Xuehong; Qiu, Hong; Teng, Michael; Azadi, Parastoo; Zhang, Fuming; Linhardt, Robert J.; Wang, Lianchun (Multidisciplinary Digital Publishing Institute (MDPI), 2021-06-11)
      The severe acute respiratory syndrome (SARS)-like coronavirus disease (COVID-19) is caused by SARS-CoV-2 and has been a serious threat to global public health with limited treatment. Cellular heparan sulfate (HS) has been ...

    • Heparan sulfates from bat and human lung and their binding to the spike protein of SARS-CoV-2 virus 

      Yan, Lufeng; Song, Yuefan; Xia, Ke; He, Peng; Zhang, Fuming; Chen, Shiguo; Pouliot, Robert; Weiss, Daniel J.; Tandon, Ritesh; Bates, John T.; Ederer, Dallas R.; Mitra, Dipanwita; Sharma, Poonam; Davis, April; Linhardt, Robert J. (2021-05-15)
      Severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2) has resulted in a pandemic and continues to spread at an unprecedented rate around the world. Although a vaccine has recently been approved, there are ...

    • Heparin Accelerates Gelsolin Amyloidogenesis 

      Suk, J.Y.; Zhang, F.; Balch, W.E.; Linhardt, Robert J.; Kelly, J.W. (American Chemical Society (ACS), 2006)
      The chemical environment of the extracellular matrix may influence the tissue-selective deposition observed there in gelsolin amyloid disease. Previously, we have identified the proteases that generate the amyloidogenic ...